Neurosteroids modulate calcium currents in hippocampal CA1 neurons via a pertussis toxin-sensitive G-protein-coupled mechanism.
Authorsffrench-Mullen JM, Danks P, Spence KT
JournalJ Neurosci
PubMed ID8158251
'The inhibition of Ca2+ channel currents by endogenous brain steroids was examined in freshly dissociated pyramidal neurons from the adult guinea pig hippocampal CA1 region. The steady-state inhibition of the peak Ca2+ channel current evoked by depolarizing steps from -80 to -10 mV occurred in a concentration-dependent manner with the ... More
Calcium-induced inactivation of NMDA receptor-channels evolves independently of run-down in cultured rat brain neurones.
AuthorsMedina I, Filippova N, Bakhramov A, Bregestovski P
JournalJ Physiol
PubMed ID8887753
'1. Calcium-induced transient inactivation of NMDA receptor (NMDAR) channels was studied in cultured rat hippocampal and cerebellar granule neurones using patch-clamp techniques and confocal scanning microscopy. 2. During whole-cell recordings, in the presence of 2 mM external Ca2+, conditioning (2-20 s) pulses of NMDA (20-100 microM) caused a transient decrease ... More
A store-operated calcium channel in Drosophila S2 cells.
AuthorsYeromin AV, Roos J, Stauderman KA, Cahalan MD
JournalJ Gen Physiol
PubMed ID14744989
'Using whole-cell recording in Drosophila S2 cells, we characterized a Ca(2+)-selective current that is activated by depletion of intracellular Ca2+ stores. Passive store depletion with a Ca(2+)-free pipette solution containing 12 mM BAPTA activated an inwardly rectifying Ca2+ current with a reversal potential >60 mV. Inward currents developed with a ... More
Ca2+ store dynamics determines the pattern of activation of the store-operated Ca2+ current I(CRAC) in response to InsP3 in rat basophilic leukaemia cells.
AuthorsGlitsch MD, Parekh AB
JournalJ Physiol
PubMed ID10699074
'1. The relationship between the amplitude of the store-operated Ca2+ ICR AC and intracellular inositol 1,4,5-triphosphate (InsP3) concentration is complex. In rat basophilic leukaemia (RBL-1) cells dialysed with high intracellular Ca2+ buffer, the relationship is supra-linear with a Hill coefficient of 12 and resembles an apparent ''all-or-none'' phenomenon. The non-linearity ... More
Phosphorylation of RIM1alpha by PKA triggers presynaptic long-term potentiation at cerebellar parallel fiber synapses.
'Presynaptic activation of protein kinase A (PKA) induces LTP in cerebellar parallel fiber synapses. Presynaptic LTP is known to require the active zone protein RIM1alpha, but the underlying induction mechanism remains unclear. We now show that PKA directly phosphorylates RIM1alpha at two sites. Using paired recordings from cultured cerebellar granule ... More
A novel monovalent cation channel activated by inositol trisphosphate in the plasma membrane of rat megakaryocytes.
AuthorsSomasundaram B, Mahaut-Smith MP
JournalJ Biol Chem
PubMed ID7542650
'The activation of a monovalent cation current was studied in rat megakaryocytes using patch clamp techniques combined with photometric measurements of intracellular concentrations of Ca2+ ([Ca2+]i) and Na+. ADP evoked a release of [Ca2+]i and transiently activated a monovalent cation-selective channel, which, at negative potentials and under physiological conditions, would ... More
A practical guide to the preparation of Ca2+ buffers.
AuthorsBers DM, Patton CW, Nuccitelli R
JournalMethods Cell Biol
PubMed ID8201981
Depletion of intracellular calcium stores by calcium ionophore A23187 induces the genes for glucose-regulated proteins in hamster fibroblasts.
AuthorsDrummond IA, Lee AS, Resendez E, Steinhardt RA
JournalJ Biol Chem
PubMed ID3114264
Animal cells respond to calcium ionophore (A23187) treatment with the coordinate induction of a set of genes encoding proteins identical to the glucose-regulated proteins (GRPs). By monitoring the intracellular free calcium with the fluorescent indicator fura-2 while employing both intracellular and extracellular calcium buffers, we demonstrated that A23187 can induce ... More
Structural determinants of allosteric regulation in alternatively spliced AMPA receptors.
AuthorsPartin KM, Bowie D, Mayer ML
JournalNeuron
PubMed ID7718245
The flip and flop splice variants of AMPA receptors show strikingly different sensitivity to allosteric regulation by cyclothiazide; heteromers assembled from GluR-A and GluR-B also exhibit splice variant-dependent differences in efficacy for activation by glutamate and kainate. The sensitivity for attenuation of desensitization by cyclothiazide for homomeric GluR-A was solely ... More
Inhibition of low threshold calcium channels by angiotensin II in adrenal glomerulosa cells through activation of protein kinase C.
AuthorsRossier MF, Aptel HB, Python CP, Burnay MM, Vallotton MB, Capponi AM
JournalJ Biol Chem
PubMed ID7797497
In adrenal glomerulosa cells, low threshold voltage-activated (T-type) calcium channels play a crucial role in coupling physiological variations of extracellular potassium to aldosterone biosynthesis. Angiotensin II markedly reduced the activity of these channels by shifting their activation curve toward positive voltage values. This inhibition of the channels resulted in a ... More
Fundamental Ca2+ signaling mechanisms in mouse dendritic cells: CRAC is the major Ca2+ entry pathway.
AuthorsHsu Sf O'Connell PJ, Klyachko VA, Badminton MN, Thomson AW, Jackson MB, Clapham DE, Ahern GP
JournalJ Immunol
PubMed ID11342632
Although Ca(2+)-signaling processes are thought to underlie many dendritic cell (DC) functions, the Ca(2+) entry pathways are unknown. Therefore, we investigated Ca(2+)-signaling in mouse myeloid DC using Ca(2+) imaging and electrophysiological techniques. Neither Ca(2+) currents nor changes in intracellular Ca(2+) were detected following membrane depolarization, ruling out the presence of ... More
Role of the phospholipase C-inositol 1,4,5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry.
We investigated the putative roles of phospholipase C, polyphosphoinositides, and inositol 1,4,5-trisphosphate (IP(3)) in capacitative calcium entry and calcium release-activated calcium current (I(crac)) in lacrimal acinar cells, rat basophilic leukemia cells, and DT40 B-lymphocytes. Inhibition of phospholipase C with blocked calcium entry and I(crac) activation whether in response to a ... More
Properties of a native cation channel activated by Ca2+ store depletion in vascular smooth muscle cells.
AuthorsTrepakova ES, Gericke M, Hirakawa Y, Weisbrod RM, Cohen RA, Bolotina VM
JournalJ Biol Chem
PubMed ID11113149
Depletion of intracellular Ca(2+) stores activates capacitative Ca(2+) influx in smooth muscle cells, but the native store-operated channels that mediate such influx remain unidentified. Recently we demonstrated that calcium influx factor produced by yeast and human platelets with depleted Ca(2+) stores activates small conductance cation channels in excised membrane patches ... More
Fcgamma receptor I activation triggers a novel Ca2+-activated current selective for monovalent cations in the human monocytic cell line, U937.
AuthorsFloto RA, Somasundaram B, Allen JM, Mahaut-Smith MP
JournalJ Biol Chem
PubMed ID9030528
Previous reports have suggested that receptors for immunoglobulin G (IgG), FcgammaRs, directly activate a nonselective cation channel (Young, J. D.-E., Unkeless, J. C., Young, T. M., Mauro, A., and Cohn, Z. A. (1983) Nature 306, 186-189; Nelson, D. J., Jacobs, E. R., Tang, J. M., Zeller, J. M., and Bone, ... More
Role of the inositol 1,4,5-trisphosphate receptor in Ca(2+) feedback inhibition of calcium release-activated calcium current (I(crac)).
AuthorsBroad LM, Armstrong DL, Putney JW
JournalJ Biol Chem
PubMed ID10551852
We examined the activation and regulation of calcium release-activated calcium current (I(crac)) in RBL-1 cells in response to various Ca(2+) store-depleting agents. With [Ca(2+)](i) strongly buffered to 100 nM, I(crac) was activated by ionomycin, thapsigargin, inositol 1,4,5-trisphosphate (IP(3)), and two metabolically stable IP(3) receptor agonists, adenophostin A and L-alpha-glycerophospho-D-myoinositol-4,5-bisphosphate (GPIP(2)). ... More
Cortisol inhibition of calcium currents in guinea pig hippocampal CA1 neurons via G-protein-coupled activation of protein kinase C.
Authorsffrench-Mullen JM
JournalJ Neurosci
PubMed ID7823188
The inhibition of voltage-activated Ca2+ channel currents by cortisol (hydrocortisone), the principal glucocorticoid in man and guinea pig, was examined in freshly dissociated pyramidal neurons from the adult guinea pig hippocampal CA1 region using whole-cell voltage-clamp recordings. Steady-state inhibition by cortisol of the peak Ca2+ channel current evoked by depolarization ... More
Snx14 regulates neuronal excitability, promotes synaptic transmission, and is imprinted in the brain of mice.
Authors
JournalPLoS One
PubMed ID24859318
Macromolecular and electrical coupling between inner hair cells in the rodent cochlea.
Authors
JournalNat Commun
PubMed ID32587250
High-Frequency Activation of Nucleus Accumbens D1-MSNs Drives Excitatory Potentiation on D2-MSNs.
Authors
JournalNeuron
PubMed ID31221559
Distinct pharmacological profiles of ORAI1, ORAI2, and ORAI3 channels.