A practical guide to the preparation of Ca2+ buffers.
AuthorsBers DM, Patton CW, Nuccitelli R
JournalMethods Cell Biol
PubMed ID8201981
TRPC1 and TRPC5 form a novel cation channel in mammalian brain.
AuthorsStrübing C, Krapivinsky G, Krapivinsky L, Clapham DE
JournalNeuron
PubMed ID11301024
TRP proteins are cation channels responding to receptor-dependent activation of phospholipase C. Mammalian (TRPC) channels can form hetero-oligomeric channels in vitro, but native TRPC channel complexes have not been identified to date. We demonstrate here that TRPC1 and TRPC5 are subunits of a heteromeric neuronal channel. Both TRPC proteins have ... More
Time-resolved charge movements in the sarcoplasmatic reticulum Ca-ATPase.
AuthorsPeinelt C, Apell HJ
JournalBiophys J
PubMed ID14747317
The time-resolved kinetics of the Ca(2+)-translocating partial reaction of the sarcoplasmatic reticulum Ca-ATPase was investigated by ATP-concentration jump experiments. ATP was released by an ultraviolet light flash from its inactive precursor and charge movements in the membrane domain of the ion pumps were detected by the fluorescent styryl dye 2BITC. ... More