Ivermectin excretion by isolated functionally intact brain endothelial capillaries.
AuthorsNobmann S, Bauer B, Fricker G
JournalBr J Pharmacol
PubMed ID11159725
'1. Functionally intact brain endothelial capillaries were isolated from porcine brain. p-Glycoprotein was localized at the lumenal membrane of intact capillaries by immunohistochemistry using a murine monoclonal antibody and a secondary FITC fluorescent labelled anti-mouse IgG. Western blot staining of p-glycoprotein in isolated endothelial cells confirmed the immunohistochemistry. 2. Excretion ... More
Xenobiotic efflux pumps in isolated fish brain capillaries.
AuthorsMiller DS, Graeff C, Droulle L, Fricker S, Fricker G
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID11742838
'To identify specific transporters that drive xenobiotics from the central nervous system to blood, the accumulation of fluorescent drugs was studied in isolated capillaries from killifish and dogfish shark brain using confocal microscopy and quantitative image analysis. In killifish brain capillaries, luminal accumulation of fluorescent derivatives of cyclosporin A and ... More
Distribution of a fluorescent ivermectin probe, bodipy ivermectin, in tissues of the nematode parasite Ascaris suum.
AuthorsMartin RJ, Kusel JR, Robertson SJ, Minta A, Haugland RP
JournalParasitol Res
PubMed ID1409535
'A fluorescent derivative of the anthelmintic ivermectin (4''''-5,7-dimethyl bodipy proprionylivermectin, referred to hereafter as bodipy ivermectin) was synthesized for an investigation of the distribution of avermectins. Injected into adult Ascaris suum at doses greater than 0.1 micron per worm, it produced a dose-dependent immobilization. Fluorescent microscopy of frozen sections revealed ... More
On the distribution of a fluorescent ivermectin probe (4" 5,7 dimethyl-bodipy proprionylivermectin) in Ascaris membranes.
AuthorsMartin RJ, Kusel JR
JournalParasitology
PubMed ID1641253
The distribution of the fluorescent ivermectin derivative (4" 5,7 dimethyl bodipy proprionylivermectin, referred to here as bodipy ivermectin) was studied in muscle vesicle membranes from the parasite Ascaris suum. Incubation in bodipy ivermectin, at concentrations greater than 1 ng/ml, produced a dose-dependent fluorescence in the vesicles. The level of fluorescence ... More
Surface properties of membrane vesicles prepared from muscle cells of Ascaris suum.
AuthorsMartin RJ, Kusel JR, Pennington AJ
JournalJ Parasitol
PubMed ID1693673
To facilitate biochemical, pharmacological, and biophysical studies on the membrane of the body muscle of Ascaris suum, a method for preparing intact vesicles was developed. Vesicles were prepared by incubating a muscle flap preparation with 1 mg/ml collagenase in a saline solution and then washing in saline without enzyme. The ... More
Epithelial transport of anthelmintic ivermectin in a novel model of isolated proximal kidney tubules.
AuthorsFricker G, Gutmann H, Droulle A, Drewe J, Miller DS
JournalPharm Res
PubMed ID10554099
PURPOSE: The mechanism of excretion of the anthelmintic drug ivermectin was investigated in a novel experimental model of functionally intact proximal tubules isolated from a teleost fish (Fundulus heteroclitus). METHODS: Secretion into the lumens of freshly isolated proximal tubules was studied by means of confocal laser scanning microscopy and digital ... More