Chemical two-photon uncaging: a novel approach to mapping glutamate receptors.
AuthorsPettit DL, Wang SS, Gee KR, Augustine GJ
JournalNeuron
PubMed ID9331338
Functional mapping of neurotransmitter receptors requires rapid and localized application of transmitter. The usefulness of caged glutamate for this purpose has been limited, because photolysis by unfocused light above and below the target cell limits depth resolution. This problem is eliminated by using a double-caged glutamate that requires absorption of ... More
Distribution of functional glutamate and GABA receptors on hippocampal pyramidal cells and interneurons.
AuthorsPettit DL, Augustine GJ
JournalJ Neurophysiol
PubMed ID10899180
The distribution of functional neurotransmitter receptors is an important determinant of neuronal information processing. To map the location of functional glutamate and GABA receptors on individual hippocampal neurons, we photolyzed "caged" glutamate and GABA while measuring the electrical currents resulting from activation of these receptors. Responses to uncaged neurotransmitters were ... More
Quantification of spread of cerebellar long-term depression with chemical two-photon uncaging of glutamate.
AuthorsWang SS, Khiroug L, Augustine GJ
JournalProc Natl Acad Sci U S A
PubMed ID10890882
Localized, chemical two-photon photolysis of caged glutamate was used to map the changes in alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid-type glutamate receptors caused by long-term synaptic depression (LTD) in cerebellar Purkinje cells. LTD produced by pairing parallel fiber activity with depolarization was accompanied by a decline in the response of Purkinje cells to uncaged ... More