Manipulating FRET with polymeric vesicles: development of a "mix-and-detect" type fluorescence sensor for bacterial toxin.
AuthorsMa G, Cheng Q
JournalLangmuir
PubMed ID16863214
'A simple "mix-and-detect" type of fluorescence sensor for cholera toxin (CT) is reported. The sensor consists of a BODIPY lipid dye and polydiacetylene (PDA) vesicles and utilizes the lipid insertion and FRET mechanism to offer a direct and fluorescence "turn-on" detection of the analyte. BODIPY conjugated GM1, dissolved in a ... More
Ganglioside partitioning and aggregation in phase-separated monolayers characterized by bodipy GM1 monomer/dimer emission.
AuthorsCoban O, Burger M, Laliberte M, Ianoul A, Johnston LJ,
JournalLangmuir
PubMed ID17477552
'The distribution of Bodipy GM1 in monolayers of binary and ternary lipid mixtures with coexisting fluid and ordered phases has been examined using a combination of atomic force microscopy and near-field scanning optical microscopy. Monolayers deposited at high (30 mN/m) and low (5 or 10 mN/m) surface pressures were examined ... More
T cell receptor can be recruited to a subset of plasma membrane rafts, independently of cell signaling and attendantly to raft clustering.
AuthorsGiurisato E, McIntosh DP, Tassi M, Gamberucci A, Benedetti A
JournalJ Biol Chem
PubMed ID12499387
'The constitutive/inducible association of the T cell receptor (TCR) with isolated detergent-resistant, lipid raft-derived membranes has been studied in Jurkat T lymphocytes. Membranes resistant to 1% Triton X-100 contained virtually no CD3epsilon, part of the TCR complex, irrespective of cell stimulation. On the other hand, membranes resistant either to a ... More
Correlated fluorescence-atomic force microscopy of membrane domains: structure of fluorescence probes determines lipid localization.
AuthorsShaw JE, Epand RF, Epand RM, Li Z, Bittman R, Yip CM
JournalBiophys J
PubMed ID16361347
'Coupling atomic force microscopy (AFM) with high-resolution fluorescence microscopy is an attractive means of identifying membrane domains by both physical topography and fluorescence. We have used this approach to study the ability of a suite of fluorescent molecules to probe domain structures in supported planar bilayers. These included BODIPY-labeled ganglioside, ... More
Applications of BODIPY-sphingolipid analogs to study lipid traffic and metabolism in cells.
AuthorsPagano RE, Watanabe R, Wheatley C, Dominguez M
JournalMethods Enzymol
PubMed ID11070900
Binding of laminin-1 to monosialoganglioside GM1 in lipid rafts is crucial for neurite outgrowth.
AuthorsIchikawa N, Iwabuchi K, Kurihara H, Ishii K, Kobayashi T, Sasaki T, Hattori N, Mizuno Y, Hozumi K, Yamada Y, Arikawa-Hirasawa E,
JournalJ Cell Sci
PubMed ID19118221
Laminin-1, an extracellular matrix molecule, promotes neurite outgrowth through the interaction of integrin and actin. Monosialoganglioside GM1 in the lipid rafts associates with and activates the NGF receptor TrkA, and enhances neurite outgrowth. However, the role of GM1 in laminin-1-induced neurite outgrowth was still unclear. Here, we describe that laminin-1 ... More
Clustering and lateral concentration of raft lipids by the MAL protein.
AuthorsMagal LG, Yaffe Y, Shepshelovich J, Aranda JF, de Marco Mdel C, Gaus K, Alonso MA, Hirschberg K,
JournalMol Biol Cell
PubMed ID19553470
MAL, a compact hydrophobic, four-transmembrane-domain apical protein that copurifies with detergent-resistant membranes is obligatory for the machinery that sorts glycophosphatidylinositol (GPI)-anchored proteins and others to the apical membrane in epithelia. The mechanism of MAL function in lipid-raft-mediated apical sorting is unknown. We report that MAL clusters formed by two independent ... More
Diffusion analysis within single nanometric apertures reveals the ultrafine cell membrane organization.
We describe the development of a new methodology to probe the plasma membrane organization of living cells at the nanometric scale. Single nanometric apertures in a metallic film limit the observed membrane area below the optical diffraction barrier. The new approach performs fluorescence correlation spectroscopy with increasing aperture sizes and ... More
Cholesterol-dependent formation of GM1 ganglioside-bound amyloid beta-protein, an endogenous seed for Alzheimer amyloid.
AuthorsKakio A, Nishimoto SI, Yanagisawa K, Kozutsumi Y, Matsuzaki K
JournalJ Biol Chem
PubMed ID11342534
GM1 ganglioside-bound amyloid beta-protein (GM1/Abeta), found in brains exhibiting early pathological changes of Alzheimer's disease (AD) including diffuse plaques, has been suggested to be involved in the initiation of amyloid fibril formation in vivo by acting as a seed. To elucidate the molecular mechanism underlying GM1/Abeta formation, the effects of ... More
Sterol structure determines the separation of phases and the curvature of the liquid-ordered phase in model membranes.
AuthorsBacia K, Schwille P, Kurzchalia T
JournalProc Natl Acad Sci U S A
PubMed ID15722414
The existence of lipid rafts in biological membranes in vivo is still debated. In contrast, the formation of domains in model systems has been well documented. In giant unilamellar vesicles (GUVs) prepared from ternary mixtures of dioleoyl-phosphatidylcholine/sphingomyelin/cholesterol, a clear separation of liquid-disordered and sphingomyelin-enriched, liquid-ordered phases could be observed. This ... More
Fluorescence correlation spectroscopy diffusion laws to probe the submicron cell membrane organization.
AuthorsWawrezinieck L, Rigneault H, Marguet D, Lenne PF
JournalBiophys J
PubMed ID16199500
To probe the complexity of the cell membrane organization and dynamics, it is important to obtain simple physical observables from experiments on live cells. Here we show that fluorescence correlation spectroscopy (FCS) measurements at different spatial scales enable distinguishing between different submicron confinement models. By plotting the diffusion time versus ... More
Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor.
AuthorsJanes PW, Ley SC, Magee AI
JournalJ Cell Biol
PubMed ID10525547
The role of lipid rafts in T cell antigen receptor (TCR) signaling was investigated using fluorescence microscopy. Lipid rafts labeled with cholera toxin B subunit (CT-B) and cross-linked into patches displayed characteristics of rafts isolated biochemically, including detergent resistance and colocalization with raft-associated proteins. LCK, LAT, and the TCR all ... More
Dynamic sequestration of the recycling compartment by classical protein kinase C.
AuthorsIdkowiak-Baldys J, Becker KP, Kitatani K, Hannun YA
JournalJ Biol Chem
PubMed ID16751194
It has been previously shown that upon sustained stimulation (30-60 min) with phorbol esters, protein kinase C (PKC) alpha and betaII become sequestered in a juxtanuclear region, the pericentrion. The activation of PKC also results in sequestration of transferrin, suggesting a role for PKC in regulating endocytosis and sequestration of ... More
Identification of a novel apical sorting motif and mechanism of targeting of the M2 muscarinic acetylcholine receptor.
AuthorsChmelar RS, Nathanson NM
JournalJ Biol Chem
PubMed ID16968700
Previous studies have shown that the M2 receptor is localized at steady state to the apical domain in Madin-Darby canine kidney (MDCK) epithelial cells. In this study, we identify the molecular determinants governing the localization and the route of apical delivery of the M2 receptor. First, by confocal analysis of ... More
Dimers of dipyrrometheneboron difluoride (BODIPY) with light spectroscopic applications in chemistry and biology.
AuthorsBergström F, Mikhalyov I, Hägglöf P, Wortmann R, Ny T, Johansson LB
JournalJ Am Chem Soc
PubMed ID11782171
A ground-state dimer (denoted D(I)) exhibiting a strong absorption maximum at 477 nm (epsilon = 97 000 M(-1)cm(-1)) can form between adjacent BODIPY groups attached to mutant forms of the protein, plasminogen activator inhibitor type 1 (PAI-1). No fluorescence from excited D(I) was detected. A locally high concentration of BODIPY ... More
Alterations in the properties of the cell membrane due to glycosphingolipid accumulation in a model of Gaucher disease.
Authors
JournalSci Rep
PubMed ID29317695
Lipid accumulation controls the balance between surface connection and scission of caveolae.