Biomembrane templates for nanoscale conduits and networks.
AuthorsEvans E,Bowman H,Leung A,Needham D,Tirrell D
JournalScience (New York, N.Y.)
PubMed ID8688071
Use of avidin-biotin technology for liposome targeting.
AuthorsRivnay B, Bayer EA, Wilchek M
JournalMethods Enzymol
PubMed ID3695955
Tracking of quantum dot-labeled CFTR shows near immobilization by C-terminal PDZ interactions.
AuthorsHaggie PM, Kim JK, Lukacs GL, Verkman AS
JournalMol Biol Cell
PubMed ID16987954
'Mutations in cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-regulated chloride channel, cause cystic fibrosis. To investigate interactions of CFTR in living cells, we measured the diffusion of quantum dot-labeled CFTR molecules by single particle tracking. In multiple cell lines, including airway epithelia, CFTR diffused little in the plasma membrane, ... More
Restriction of receptor movement alters cellular response: physical force sensing by EphA2.
AuthorsSalaita K, Nair PM, Petit RS, Neve RM, Das D, Gray JW, Groves JT,
JournalScience
PubMed ID20223987
'Activation of the EphA2 receptor tyrosine kinase by ephrin-A1 ligands presented on apposed cell surfaces plays important roles in development and exhibits poorly understood functional alterations in cancer. We reconstituted this intermembrane signaling geometry between live EphA2-expressing human breast cancer cells and supported membranes displaying laterally mobile ephrin-A1. Receptor-ligand binding, ... More
On the mode of liposome-cell interactions. Biotin-conjugated lipids as ultrastructural probes.
AuthorsBayer EA, Rivnay B, Skutelsky E
JournalBiochim Biophys Acta
PubMed ID420828
'An efficient method for labeling and visualizing phospholipids at the ultrastructural level is described. Biotin was coupled to the amines of appropriate phospholipids via the N-hydroxysuccinimide ester. The biotinylated lipid could be specifically labeled by ferritin-avidin conjugates and detected by transmission electron microscopy. The lipid derivatives were analyzed and evaluated ... More
Avidin attachment to red blood cells via a phospholipid derivative of biotin provides complement-resistant immunoerythrocytes.
AuthorsMuzykantov VR, Smirnov MD, Klibanov AL
JournalJ Immunol Methods
PubMed ID8429223
'Preincubation of red blood cells (RBC) in an aqueous dispersion of biotin-phosphatidylethanolamine (biotin-PE) provides binding sites for avidin on the surface of these cells (up to 5 x 10(5) avidin molecules per cell). Previously we have shown that biotin covalently attached to the surface of RBC by a chemical reaction ... More
Higher order self-assembly of vesicles by site-specific binding.
'The association of lipid molecules into spherical vesicles in solution as a result of non-specific intermolecular forces constitutes a primary self-assembly process. Such vesicles can undergo a secondary self-assembly into higher order structures in a controlled and reversible manner by means of site-specific ligand-receptor (biotin-streptavidin) coupling. Cryoelectron microscopy shows these ... More
Fluorescence studies on the mechanism of liposome-cell interactions in vitro.
AuthorsSzoka F, Jacobson K, Derzko Z, Papahadjopoulos D
JournalBiochim Biophys Acta
PubMed ID7397162
'Sonicated unilamellar liposomes containing fluorescent lipid analogs or biotinyl phosphatidylethanolamine as a ligand for fluorescein avidin have been used to study the mechanism of interaction of phospholipid vesicles with eucaryotic cells. Microscopy revealed that after short incubations the fluorescence was associated with the cell surface in a punctate as opposed ... More
Membrane anchorage brings about fusogenic properties in a short synthetic peptide.
AuthorsPécheur EI, Hoekstra D, Sainte-Marie J, Maurin L, Bienvenüe A, Philippot JR
JournalBiochemistry
PubMed ID9092806
'The fusogenic properties of an amphipathic net-negative peptide (wae 11), consisting of 11 amino acid residues, were studied. We demonstrate that, whereas the free peptide displays no significant fusion activity, membrane fusion is strongly promoted when the peptide is anchored to a liposomal membrane. The fusion activity of the peptide ... More
A mechanism for stabilization of membranes at low temperatures by an antifreeze protein.
AuthorsTomczak MM, Hincha DK, Estrada SD, Wolkers WF, Crowe LM, Feeney RE, Tablin F, Crowe JH
JournalBiophys J
PubMed ID11806929
'Polar fish, cold hardy plants, and overwintering insects produce antifreeze proteins (AFPs), which lower the freezing point of solutions noncolligatively and inhibit ice crystal growth. Fish AFPs have been shown to stabilize membranes and cells in vitro during hypothermic storage, probably by interacting with the plasma membrane, but the mechanism ... More
Design of a glucose minisensor based on streptavidin-glucose oxidase complex coupling with self-assembled biotinylated phospholipid membrane on solid support.
AuthorsSnejdárková M, Rehák M, Otto M
JournalAnal Chem
PubMed ID8460828
'A simple and fast procedure to prepare a glucose-sensitive minielectrode is presented. It is based on a biotin-modified phospholipid bilayer to which a streptavidin-glucose oxidase complex is coupled. The assay is based on the electrochemical detection of enzymatically generated hydrogen peroxide at the potential +670 mV. In the case of ... More
Protein-liposome conjugates with defined size distributions.
AuthorsLoughrey HC, Wong KF, Choi LS, Cullis PR, Bally MB
JournalBiochim Biophys Acta
PubMed ID2207121
'Conjugation of protein to liposomes by two coupling protocols is shown to result in vesicle aggregation. The degree of aggregation is directly related to the levels of protein conjugated to the liposomes. In an attempt to develop a method of generating stable, homogeneously sized protein-conjugated vesicles, highly aggregated liposome-protein conjugates ... More
Antibody-antigen binding constants determined in solution-phase with the threshold membrane-capture system: binding constants for anti-fluorescein, anti-saxitoxin, and anti-ricin antibodies.
AuthorsDill K, Lin M, Poteras C, Fraser C, Hafeman DG, Owicki JC, Olson JD
JournalAnal Biochem
PubMed ID8203727
Affinities of various monoclonal and polyclonal antibodies for fluorescein-containing antigens, saxitoxin and ricin, were determined by using a light addressable potentiometric sensor-based system (Threshold). The dissociation constants, determined from Scatchard plots, ranged from 2 x 10(-7) to approximately 3 x 10(-12) M. Dissociation constants for fluorescein and saxitoxin were compared ... More
Two-dimensional crystallization of avidin on biotinylated lipid monolayers.
AuthorsQin H, Liu Z, Sui SF
JournalBiophys J
PubMed ID7647251
Two-dimensional crystals of avidin were obtained on mixed lipid monolayers containing biotinylated lipids (N-biotinyl-dipalmitoyl-L-alpha-phosphatidyl ethanolamine and dioleoyl phosphatidyl choline) by specific interaction. Image analysis of electron micrographs of these crystals revealed p2 symmetry with the unit cell parameters a = 66 +/- 2 A, b = 68 +/- 1 A, ... More
Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules.
AuthorsDarst SA, Ahlers M, Meller PH, Kubalek EW, Blankenburg R, Ribi HO, Ringsdorf H, Kornberg RD
JournalBiophys J
PubMed ID1901232
Streptavidin forms two-dimensional crystals when specifically bound to layers of biotinylated lipids at the air/water interface. The three-dimensional structure of streptavidin determined from the crystals by electron crystallography corresponds well with the structure determined by x-ray crystallography. Comparison of the electron and x-ray crystallographic structures reveals the occurrence of free ... More
A non-covalent method of attaching antibodies to liposomes.
AuthorsLoughrey H, Bally MB, Cullis PR
JournalBiochim Biophys Acta
PubMed ID3593723
A novel non-covalent method of attaching antibodies to liposomes which exploits the high affinity of streptavidin for biotin, is described. The two-step coupling protocol involves the initial attachment of streptavidin to liposomes containing biotin PE, followed by the coupling of biotinated antibodies to streptavidin-liposomes. The association of streptavidin with liposomes ... More
Europium chelate-loaded liposomes: a tool for the study of binding and integrity of liposomes.
AuthorsOrellana A, Laukkanen ML, Keinänen K
JournalBiochim Biophys Acta
PubMed ID8865811
Using the biotin-streptavidin interaction as a model, we investigated the suitability of lanthanide chelates as encapsulated liposomal labels in liposome-based binding assays. Large unilamellar phospholipid:cholesterol liposomes containing europium-DTPA chelate and biotinylated phosphatidylethanolamine were prepared by detergent dialysis. The resulting Eu-liposomes ([symbol: see text] 120 nm) bound specifically to streptavidin in ... More
Generic liposome reagent for immunoassays.
AuthorsPlant AL, Brizgys MV, Locasio-Brown L, Durst RA
JournalAnal Biochem
PubMed ID2742132
We have derivatized liposomes with antibodies by using avidin to crosslink biotinylated phospholipid molecules in the liposome membranes with biotinylated antibody molecules. A comparison of the biotin binding activity of avidin in solution and avidin associated with liposomes shows that avidin bound to biotinylated phospholipid in liposome membranes retains full ... More
Encapsulation of bilayer vesicles by self-assembly.
AuthorsWalker SA, Kennedy MT, Zasadzinski JA
JournalNature
PubMed ID9139822
Vesicles of lipid bilayers have been investigated as drug-delivery vehicles for almost 20 years. The vesicles' interior space is separated from the surrounding solution because small molecules have only limited permeability through the bilayer. Single-walled (unilamellar) vesicles are made by a variety of non-equilibrium techniques, including mechanical disruption of lamellar ... More
Synthesis of the chelator lipid nitrilotriacetic acid ditetradecylamine (NTA-DTDA) and its use with the IAsys biosensor to study receptor-ligand interactions on model membranes.
AuthorsAltin JG, White FA, Easton CJ
JournalBiochim Biophys Acta
PubMed ID11470085
This work describes the synthesis and use of the chelator lipid, nitrilotriacetic acid ditetradecylamine (NTA-DTDA). This lipid is readily dispersed in aqueous media, both alone and when mixed with carrier lipids like dimyristoylphosphatidylcholine (DMPC). Fluorescence microscopic examination of membranes deposited from NTA-DTDA-containing liposomes shows that NTA-DTDA mixes uniformly with the ... More
Structure of vitaminylated lipids in aqueous dispersion: X-ray diffraction and 31P NMR studies of N-biotinylphosphatidylethanolamines.
AuthorsSwamy MJ, Würz U, Marsh D
JournalBiochemistry
PubMed ID8399165
The structures of the phases formed in excess buffer (at pH 7.4) by a homologous series of saturated diacylphosphatidylethanolamines in which the headgroup is N-derivatized with biotin have been investigated for chain lengths of C(12:0) to C(20:0), using both 31P nuclear magnetic resonance (NMR) spectroscopy and small-angle X-ray diffraction. In ... More
Tracking movements of lipids and Thy1 molecules in the plasmalemma of living fibroblasts by fluorescence video microscopy with nanometer scale precision.
AuthorsHicks BW, Angelides KJ
JournalJ Membr Biol
PubMed ID7658460
The lateral diffusion of 100 nm fluorescent latex microspheres (FS) bound to either N-biotinyl-phosphatidyl-ethanolamine or the glycosylphosphatidylinositol-linked protein Thy1 were monitored in the plasmalemma of primary rat fibroblasts by single particle tracking of FS centroids from digital fluorescence micrographs. A silicon intensified target camera was found to be superior to ... More
Differential scanning calorimetry of thermotropic phase transitions in vitaminylated lipids: aqueous dispersions of N-biotinyl phosphatidylethanolamines.
AuthorsSwamy MJ, Angerstein B, Marsh D
JournalBiophys J
PubMed ID8130345
The thermotropic phase behavior of a homologous series of saturated diacyl phosphatidylethanolamines in which the headgroup is N-derivatized with biotin has been investigated by differential scanning calorimetry. In 1 M NaCl, derivatives with acyl chainlengths from C(12:0) to C(20:0) all exhibit sharp chain-melting phase transitions, which are reversible with a ... More