Design of a photoreactive analogue of the Escherichia coli heat-stable enterotoxin STIb: use in identifying its receptor on rat brush border membranes.
AuthorsGariépy J, Schoolnik GK
JournalProc Natl Acad Sci U S A
PubMed ID3510436
'The Escherichia coli heat-stable enterotoxin, STIb was prepared by solid-phase peptide synthesis and purified to homogeneity by high-pressure liquid chromatography. This analogue was iodinated and shown to bind specifically to rat intestinal membranes. The radiolabeled peptide was derivatized at the amino terminus with the photoreactive heterobifunctional crosslinking agent N-hydroxysuccinimidyl p-benzoylbenzoate. ... More
Photoaffinity labeling of central cholecystokinin receptors with high efficiency.
AuthorsThiele C, Fahrenholz F
JournalBiochemistry
PubMed ID8457542
'A new photoreactive tritiated cholecystokinin (CCK) analogue was synthesized which contains the p-benzoylbenzoyl moiety linked to an ornithine residue at the N-terminus of the sulfated CCK octapeptide (CCK-8s). p-Benzoylbenzoyl-Orn(propionyl)-CCK-8s bound specifically and with high affinity to CCK binding sites in membranes both from pig cerebral cortex and from rat pancreatic ... More
A fluorescent radioiodinated oligonucleotidic photoaffinity probe for protein labeling: synthesis and photolabeling of thrombin.
AuthorsBerens C, Courtoy PJ, Sonveaux E
JournalBioconjug Chem
PubMed ID9893964
'To study the interactions between oligonucleotides and proteins, an original photoaffinity radiolabeling probe has been synthesized. Starting with a 5''-pyridyldithio-3''-amino-oligonucleotide, the photophore benzophenone was first coupled to the 3'' end, through acylation by an activated ester of benzoylbenzoic acid. A fluorescein molecule was grafted by alkylation of the free 5''-SH. ... More
Survey of four different photoreactive moieties for DNA photoaffinity labeling of yeast RNA polymerase III transcription complexes.
AuthorsTate JJ, Persinger J, Bartholomew B
JournalNucleic Acids Res
PubMed ID9490787
In order to optimize the detection of protein-DNA contacts by DNA photoaffinity labeling, we attached four different photoreactive groups to DNA and examined their ability to crosslink yeast RNA polymerase III (Pol III) transcription complexes. Photoreactive nucleotides containing an aryl azide (AB-dUMP), benzophenone (BP-dUMP), perfluorinated aryl azide (FAB-dUMP) or diazirine ... More