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引用和文献 (87)
Invitrogen™
生物素-X，SE（6-((生物素基)氨基)己酸，琥珀酰亚胺酯（生物素氨基己酸，N-羟基琥珀酰亚胺酯）
胺反应性生物素-X，SE 可用于将该重要半抗原连接至目标生物分子，以便后续使用链霉素亲和素、亲和素或 NeutrAvidin™ 生物素结合蛋白进行检测。了解更多信息
| 货号 | 数量 |
|---|---|
| B1582 又称 B-1582 | 100 mg |
货号 B1582
又称 B-1582
价格（CNY)
4,025.00
Each
数量:
100 mg
价格（CNY)
4,025.00
Each
胺反应性生物素-X，SE 可用于将该重要半抗原连接至目标生物分子，以便后续使用链霉素亲和素、亲和素或 NeutrAvidin™ 生物素结合蛋白进行检测。
仅供科研使用。不可用于诊断程序。
规格
化学反应性胺
标签或染料生物素-X
产品类型生物素-XX SE
数量100 mg
反应一部分活性酯、琥珀酰亚胺酯
运输条件室温
标签类型生物素和其他半抗原
Unit SizeEach
内容与储存
在冰箱(-5 至 -30°C)中储存。
引用和文献 (87)
引用和文献
Abstract
Peptide biotinylation with amine-reactive esters: differential side chain reactivity.
Journal:Peptides
PubMed ID:9437720
N-hydroxysuccinimide (NHS) esters of biotin are reported to react specifically with amino groups of peptides and proteins. However, we have found that these reagents can readily acylate other functional groups in specific peptide sequences under relatively mild conditions. We have extended our inquiry of sequence-dependent acylation by evaluating the reactivity
Enumeration of antigen-specific IgE responses at the single-cell level by an ELISA plaque assay.
Journal:J Immunol Methods
PubMed ID:1703186
In vitro elicitation and enumeration of antigen-specific IgE-secreting cells in antigen-primed murine lymphocyte cultures can be reproducibly and accurately quantitated. Rat anti-mouse IgE monoclonal antibodies (Mab anti-epsilon) were developed. Antigen-specific IgE-secreting cells can be detected on antigen or MAb anti-epsilon coated nitrocellulose discs pasted to 24-well culture plates. This IgE
A novel fluorescent toxin to detect and investigate Kv1.3 channel up-regulation in chronically activated T lymphocytes.
Journal:J Biol Chem
PubMed ID:12511563
T lymphocytes with unusually high expression of the voltage-gated Kv1.3 channel (Kv1.3(high) cells) have been implicated in the pathogenesis of experimental autoimmune encephalomyelitis, an animal model for multiple sclerosis. We have developed a fluoresceinated analog of ShK (ShK-F6CA), the most potent known inhibitor of Kv1.3, for detection of Kv1.3(high) cells
Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques.
Journal:J Cell Biol
PubMed ID:1659577
'We previously demonstrated that the gap junction protein connexin43 is translated as a 42-kD protein (connexin43-NP) that is efficiently phosphorylated to a 46,000-Mr species (connexin43-P2) in gap junctional communication-competent, but not in communication-deficient, cells. In this study, we used a combination of metabolic radiolabeling and immunoprecipitation to investigate the assembly
Localized suppression of RhoA activity by Tyr31/118-phosphorylated paxillin in cell adhesion and migration.
Journal:J Cell Biol
PubMed ID:12446743
'RhoA activity is transiently inhibited at the initial phase of integrin engagement, when Cdc42- and/or Rac1-mediated membrane spreading and ruffling predominantly occur. Paxillin, an integrin-assembly protein, has four major tyrosine phosphorylation sites, and the phosphorylation of Tyr31 and Tyr118 correlates with cell adhesion and migration. We found that mutation of