High-performance liquid chromatographic determination of biotin in biological materials after crown ether-catalyzed fluorescence derivatization with panacyl bromide.
AuthorsStein J, Hahn A, Lembcke B, Rehner G
JournalAnal Biochem
PubMed ID1534471
This paper reports a high-performance liquid chromatographic (HPLC) technique to determine biotin in biological samples. Biotin and the internal standard dethiobiotin are converted into fluorescent derivatives by using panacyl bromide [p-(9-anthroyloxy)phenacyl bromide] as a fluorescence label. Biotin is extracted from biological tissue with trichloroacetic acid and the extract is purified ... More
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
AuthorsHirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP
JournalAnal Biochem
PubMed ID12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by ... More
Diaminobiotin and desthiobiotin have biotin-like activities in Jurkat cells.
AuthorsRodriguez-Melendez R, Lewis B, McMahon RJ, Zempleni J
JournalJ Nutr
PubMed ID12730407
'In mammals, biotin serves as a coenzyme for carboxylases such as propionyl-CoA carboxylase. The expression of genes encoding interleukin-2 (IL-2) and IL-2 receptor (IL-2R)gamma also depends on biotin. Biotin metabolites are structurally similar to biotin, and their concentrations in tissues are quantitatively important. Here, the hypothesis was tested that biotin ... More
Cellular uptake of biotin: mechanisms and regulation.
AuthorsSaid HM
JournalJ Nutr
PubMed ID10064315
'This review describes our knowledge of biotin transport in the small intestine of humans and other mammals and presents recent findings in the area. Previous studies have shown that biotin transport across the brush border membrane of the small intestinal absorptive cells occurs via a carrier-mediated, Na+ gradient-dependent, electroneutral mechanism. ... More
Biotin transport through the blood-brain barrier.
AuthorsSpector R, Mock D
JournalJ Neurochem
PubMed ID3098919
'The unidirectional influx of biotin across cerebral capillaries, the anatomical locus of the blood-brain barrier, was measured with an in situ rat brain perfusion technique employing [3H]biotin. Biotin was transported across the blood-brain barrier by a saturable system with a one-half saturation concentration of approximately 100 microM. The permeability-surface area ... More
A protein-binding assay for measurement of biotin in physiological fluids.
AuthorsHorsburgh T, Gompertz D
JournalClin Chim Acta
PubMed ID23227
'A sensitive and convenient protein-binding assay for biotin in physiological fluids is described. The method is based upon the binding of an iodinated biotin conjugate by avidin followed by separation of bound and free conjugate by charcoal absorption. Adult plasma biotin levels averaged 1.26 pmol/ml, a value comparable to that ... More
Peptides, antibodies, and FRET on beads in flow cytometry: A model system using fluoresceinated and biotinylated beta-endorphin.
AuthorsBuranda T, Lopez GP, Keij J, Harris R, Sklar LA
JournalCytometry
PubMed ID10451503
'BACKGROUND: Particulate surfaces such as beads are routinely used as platforms for molecular assembly for fundamental and practical applications in flow cytometry. Molecular assembly is transduced as the direct analysis of fluorescence, or as a result of fluorescence resonance energy transfer. Binding of fluorescent ligands to beads sometimes alters their ... More
Noncooperativity of biotin binding to tetrameric streptavidin.
AuthorsJones ML, Kurzban GP
JournalBiochemistry
PubMed ID7547907
'Streptavidin tetramers have been separated according to their biotin content by anion exchange chromatography. Biotin-free and biotin-saturated streptavidin were coincubated. Streptavidin at intermediate ligation levels, i.e., with one, two, or three molecules of bound biotin, accumulates over time. A steady state distribution of ligation levels is reached after 2 days. ... More
The biotin transport system.
AuthorsBayer EA, Wilchek M
JournalMethods Enzymol
PubMed ID333227
COMBINABILITY OF AVIDIN AND STREPTAVIDIN WITH ANALOGS OF BIOTIN.
AuthorsLICHSTEIN HC, BIRNBAUM J
JournalBiochem Biophys Res Commun
PubMed ID14341938
A SPECTROPHOTOMETRIC ASSAY FOR AVIDIN AND BIOTIN BASED ON BINDING OF DYES BY AVIDIN.
AuthorsGREEN NM,
JournalBiochem J
PubMed ID14340040
A spectrophotometric assay for avidin and biotin based on binding of dyes by avidin.
Irreversible inhibition of biotin transport in yeast by biotinyl-p-nitrophenyl ester.
AuthorsBecker JM, Wilchek M, Katchalski E
JournalProc Natl Acad Sci U S A
PubMed ID4944635
Biotinyl-p-nitrophenyl ester (BNP), an active-ester derivative of biotin, irreversibly inactivates biotin transport in the yeast Saccharomyces cerevisiae. Transport inactivation is progressive with time and occurs at concentrations of the ester as low as 10(-7) M. In the presence of sodium azide, a reagent known to block biotin accumulation in yeast, ... More
Lissencephaly-1 promotes the recruitment of dynein and dynactin to transported mRNAs.
Microtubule-based transport mediates the sorting and dispersal of many cellular components and pathogens. However, the mechanisms by which motor complexes are recruited to and regulated on different cargos remain poorly understood. Here we describe a large-scale biochemical screen for novel factors associated with RNA localization signals mediating minus end-directed mRNA ... More
Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer.
AuthorsTavoosidana G, Ronquist G, Darmanis S, Yan J, Carlsson L, Wu D, Conze T, Ek P, Semjonow A, Eltze E, Larsson A, Landegren UD, Kamali-Moghaddam M,
JournalProc Natl Acad Sci U S A
PubMed ID21555566
Prostasomes are microvesicles (mean diameter, 150 nm) that are produced and secreted by normal and malignant prostate acinar cells. It has been hypothesized that invasive growth of malignant prostate cells may cause these microvesicles, normally released into seminal fluid, to appear in interstitial space and therewith into peripheral circulation. The ... More
Identification of arsenic-binding proteins in human breast cancer cells.
AuthorsZhang X, Yang F, Shim JY, Kirk KL, Anderson DE, Chen X,
JournalCancer Lett
PubMed ID17499915
As a cancer chemotherapeutic drug, arsenic acts on numerous intracellular signal transduction pathways in cancer cells. However, its mechanism of actions is still not fully understood. Previous studies suggest that arsenic reacts with closely spaced cysteine (Cys) residues of proteins with high Cys content and accessible sulfhydryl (SH) groups. In ... More
Selective enrichment of thiophosphorylated polypeptides as a tool for the analysis of protein phosphorylation.
AuthorsKwon SW, Kim SC, Jaunbergs J, Falck JR, Zhao Y,
JournalMol Cell Proteomics
PubMed ID12734387
A chemoselective alkylation method is described for the isolation and subsequent identification of thiophosphorylated peptides/proteins. The method involves thiophosphorylation of proteins using adenosine 5'-O-(thiotriphosphate) (ATPgammaS) followed by selective in situ alkylation of the newly thiophosphorylated proteins resulting in a stable covalent bond. The chemoselective alkylation exploits the relatively high nucleophilicity ... More
A sensitive enzyme assay for biotin, avidin, and streptavidin.
AuthorsBayer EA, Ben-Hur H, Wilchek M
JournalAnal Biochem
PubMed ID3706735
Reciprocal enzyme assays are described for the vitamin biotin and for the biotin-binding proteins avidin and streptavidin. The assays are based on the following steps: (a) biotinylated bovine serum albumin is adsorbed onto microtiter plates; (b) streptavidin (or avidin) is bound to the biotin-coated plates; (c) biotinylated enzyme (in this ... More
Determination of biotin concentration by a competitive enzyme-linked immunosorbent assay (ELISA) method.
AuthorsChang YS, Wu CH, Chang RJ, Shiuan D
JournalJ Biochem Biophys Methods
PubMed ID7699208
A method based on competitive enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of biotin concentrations which takes advantage of the extraordinarily high affinity between biotin and avidin. The biotin assay consisted of two steps, (i) a competition reaction between excess streptavidin-conjugated horseradish peroxidase (streptavidin-HRP) and solutions of known ... More
Liposome dehydration on nitrocellulose and its application in a biotin immunoassay.
AuthorsMartorell D, Siebert ST, Durst RA
JournalAnal Biochem
PubMed ID10419633
The feasibility of utilizing dehydrated liposomes in the development of a simple immunoassay device for point-of-care diagnostics or field assays was demonstrated. The recovery of liposomes after a cycle of dehydration and rehydration was studied using biotin-tagged, dye-loaded liposomes with antibiotin antibodies immobilized in a defined zone on nitrocellulose strips. ... More