A series of biotinylated tracers distinguishes three types of gap junction in retina.
AuthorsMills SL, Massey SC
JournalJ Neurosci
PubMed ID11069972
'Gap junctions serve many important roles in various tissues, but their abundance and diversity in neurons is only beginning to be understood. The tracer Neurobiotin has revealed many different networks interconnected by gap junctions in retina. We compared the relative permeabilities of five different retinal gap junctions by measuring their ... More
Differential properties of two gap junctional pathways made by AII amacrine cells.
AuthorsMills SL, Massey SC
JournalNature
PubMed ID7477263
'The retina is sensitive to light stimuli varying over more than 12 log units in intensity. It accomplishes this, in part, by switching between rod-dominated circuits designed for maximum utilization of scarce photons and cone circuits designed for greater acuity. Rod signals are integrated into the cone pathways through AII ... More
Measurement of tissue transglutaminase activity in a permeabilized cell system: its regulation by Ca2+ and nucleotides.
AuthorsSmethurst PA, Griffin M
JournalBiochem J
PubMed ID8611158
Electropermeabilized human endothelial cells (ECV-304) were used to study the regulation of tissue transglutaminase (tTGase) activity in the intracellular environment. An ELSA (enzyme-linked sorbent assay) plate assay was developed for intracellular tTGase activity, using the incorporation of a biotinylated primary amine, 5-{[(N-biotinoylamino)hexanoyl]amino}pentylamine(biotin-x-cadaveri ne; BTC), into endogenous protein substrates of tTGase. ... More
A microtiter assay for factor XIII using fibrinogen and biotinylcadaverine as substrates.
AuthorsSong YC, Sheng D, Taubenfeld SM, Matsueda GR
JournalAnal Biochem
PubMed ID7695107
Efforts to develop an improved assay for plasma and tissue transglutaminase have led us to a convenient, sensitive microtiter plate assay for coagulation factor XIII using human fibrinogen as an immobilized substrate. Factor XIII was activated in the presence of calcium, thrombin, and immobilized fibrinogen and then assayed by adding ... More