Innate immune recognition triggers secretion of lysosomal enzymes by macrophages.
AuthorsLackman RL, Jamieson AM, Griffith JM, Geuze H, Cresswell P
JournalTraffic
PubMed ID17555533
'Gamma interferon-induced lysosomal thiolreductase (GILT) is expressed constitutively in antigen-presenting cells, where it reduces disulfide bonds to facilitate antigen presentation. GILT is synthesized as an enzymatically active precursor protein and is processed in early endosomes to yield the mature enzyme. The exposure of the promonocytic cell line THP-1 to Escherichia ... More
The radioprotective agent, amifostine, suppresses the reactivity of intralysosomal iron.
AuthorsYu Z, Eaton JW, Persson HL
JournalRedox Rep
PubMed ID14980067
Amifostine (2-[(3-aminopropyl)amino]ethane-thiol dihydrogen phosphate ester; WR-2721) is a radioprotective agent used clinically to minimize damage from radiation therapy to adjacent normal tissues. This inorganic thiophosphate requires dephosphorylation to produce the active, cell-permeant thiol metabolite, WR-1065. The activation step is presumably catalyzed by membrane-bound alkaline phosphatase, activity of which is substantially ... More