BrdUTP (5-溴-2'-脱氧尿苷 5'-三磷酸盐)，TE 缓冲液中 10 mM
BrdUTP (5-溴-2'-脱氧尿苷 5'-三磷酸盐)，TE 缓冲液中 10 mM
引用和文献 (15)
Invitrogen™

BrdUTP (5-溴-2'-脱氧尿苷 5'-三磷酸盐)，TE 缓冲液中 10 mM

BrdUTP can be used in conjunction with tetrminal deoxynucleotidyl transferase (TdT) to label apoptotic cells in the TUNEL assay. BrdUTP了解更多信息
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货号数量
B2155025 μL
货号 B21550
价格（CNY)
3,000.00
Each
添加至购物车
数量:
25 μL
价格（CNY)
3,000.00
Each
添加至购物车
BrdUTP can be used in conjunction with tetrminal deoxynucleotidyl transferase (TdT) to label apoptotic cells in the TUNEL assay. BrdUTP can be detected with anti-BrdU antibodies.
仅供科研使用。不可用于诊断程序。
规格
标签或染料其他标记或染料
产品类型BrdUTP
数量25 μL
运输条件湿冰
最大浓度10 mM
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

常见问题解答 (FAQ)

Can dU-containing DNA produced by amplification with dUTP be cut by restriction enzymes?

You will need to confirm with the manufacturer of the restriction enzyme you are using to find out whether or not it can recognize dUTP-containing DNA.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

引用和文献 (15)

引用和文献
Abstract
Immunoseparation and immunodetection of nucleic acids labeled with halogenated nucleotides.
Authors:Haider SR, Juan G, Traganos F, Darzynkiewicz Z
Journal:Exp Cell Res
PubMed ID:9260920
'A novel methodology for labeling, isolation, and detection of nucleic acids is described. Nucleic acid isolation is based on in vivo or in vitro incorporation of BrU or BrdU to either RNA or DNA, respectively, followed by immunoprecipitation of the labeled nucleic acid utilizing anti-BrdU MoAb, which crossreacts with BrU, ... More
Purification and characterization of human topoisomerase I mutants.
Authors:Jensen AD, Svejstrup JQ
Journal:Eur J Biochem
PubMed ID:8612607
'A system for rapid purification and characterization of eukaryotic topoisomerase-I mutants has been developed. The system utilizes six-histidine tagging of human topoisomerase I expressed in Saccharomyces cerevisiae to enable purification by nickel-affinity chromatography. Virtually homogenous mutant proteins are then tested for their ability to relax supercoiled DNA plasmids and their ... More
Flow cytometric evaluation of apoptosis, necrosis and recovery when culturing monocytes.
Authors:Lund PK, Westvik AB, Joø GB, Øvstebø R, Haug KB, Kierulf P
Journal:J Immunol Methods
PubMed ID:11334964
'After developing and applying a method for cryopreserving monocytes, we found a substantial cell loss when culturing these cells. Monocytes were isolated from blood donors by density gradient centrifugation, purified by elutriation and cryopreserved. Thawed cells were cultured in ultra low attachment wells and studied with Annexin V, Propidium iodide, ... More
A sensitive non-isotopic assay specific for HIV-1 associated reverse transcriptase.
Authors:Porstmann T, Meissner K, Glaser R, Döpel SH, Sydow G
Journal:J Virol Methods
PubMed ID:1713913
'A sensitive non-isotopic assay for specific detection of reverse transcriptase (RT) of the human immunodeficiency virus type 1 (HIV-1) is described using 5-bromo-2''-deoxyuridine triphosphate (BrdUTP) instead of tritiated thymidine triphosphate. After the RT reaction the template primer is degraded by alkaline hydrolysis. Single-stranded poly.(BrdU) is detected in an immunoenzymometric assay ... More
A sensitive assay for the quantification of reverse transcriptase activity based on the use of carrier-bound template and non-radioactive-product detection, with special reference to human-immunodeficiency-virus isolation.
Authors:Ekstrand DH, Awad RJ, Källander CF, Gronowitz JS
Journal:Biotechnol Appl Biochem
PubMed ID:8639277
'A non-radioactive 96-well microtitre plate reverse transcriptase (RT) assay, based on the use of covalently bound riboadenosine homopolymer in the wells and 5-bromodeoxyuridined 5''-triphosphate (BrdUTP) as dNTP, is described. The whole assay is performed in a single well, including the quantitative detection of incorporated BrdU, which is performed immunologically using ... More
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