Fluorescent BODIPY-GTP analogs: real-time measurement of nucleotide binding to G proteins.
AuthorsMcEwen DP, Gee KR, Kang HC, Neubig RR
JournalAnal Biochem
PubMed ID11262163
Three BODIPY GTPgammaS analogs (FL, 515, and TR), BODIPY FL GppNHp and BODIPY FL GTP molecules were synthesized as possible fluorescent probes to study guanine nucleotide binding spectroscopically. Binding to G(alphao) increases baseline analog fluorescence by 6-, 8.5-, 2.8-, 3.5-, and 3.0-fold, respectively. Binding of GTPgammaS and GppNHp analogs to ... More