Multiple functional P2X and P2Y receptors in the luminal and basolateral membranes of pancreatic duct cells.
AuthorsLuo X,Zheng W,Yan M,Lee MG,Muallem S
JournalThe American journal of physiology
PubMed ID10444396
Cloning and functional characterisation of the mouse P2X7 receptor.
AuthorsChessell IP, Simon J, Hibell AD, Michel AD, Barnard EA, Humphrey PP
JournalFEBS Lett
PubMed ID9849870
We have isolated a 1785-bp complementary DNA (cDNA) encoding the murine P2X7 receptor subunit from NTW8 mouse microglial cells. The encoded protein has 80%, and 85% homology to the human and rat P2X7 subunits, respectively. Functional properties of the heterologously expressed murine P2X7 homomeric receptor broadly resembled those of the ... More
Characterization and channel coupling of the P2Y(12) nucleotide receptor of brain capillary endothelial cells.
AuthorsSimon Joseph; Filippov Alexander K; Göransson Sara; Wong Yung H; Frelin Christian; Michel Anton D; Brown David A; Barnard Eric A;
JournalJ Biol Chem
PubMed ID12080041
Rat brain capillary endothelial (B10) cells express an unidentified nucleotide receptor linked to adenylyl cyclase inhibition. We show that this receptor in B10 cells is identical in sequence to the P2Y(12) ADP receptor ( ... More
Stress-activated protein kinase/JNK activation and apoptotic induction by the macrophage P2X7 nucleotide receptor.
AuthorsHumphreys BD, Rice J, Kertesy SB, Dubyak GR
JournalJ Biol Chem
PubMed ID10854431
'In human and rodent macrophages, activation of the P2X7 nucleotide receptor stimulates interleukin-1beta processing and release, apoptosis, and killing of intracellular Mycobacterium tuberculosis. Signaling pathways downstream of this ionotropic ATP receptor are poorly understood. Here we describe the rapid activation of the stress-activated protein kinase (SAPK)/JNK pathway in BAC1 murine ... More
Benzoyl ATP is an antagonist of rat and human P2Y1 receptors and of platelet aggregation.
AuthorsVigne P, Hechler B, Gachet C, Breittmayer JP, Frelin C
JournalBiochem Biophys Res Commun
PubMed ID10066429
'The effects of 2''- and 3''-O-(4-benzoylbenzoyl)-ATP (BzATP) on intracellular Ca2+ mobilization and cyclic AMP accumulation were investigated using rat brain capillary endothelial cells which express an endogenous P2Y1 receptor, human platelets which are known to express a P2Y1 receptor, and Jurkat cells stably transfected with the human P2Y1 receptor. In ... More
Extracellular ATP triggers tumor necrosis factor-alpha release from rat microglia.
AuthorsHide I, Tanaka M, Inoue A, Nakajima K, Kohsaka S, Inoue K, Nakata Y
JournalJ Neurochem
PubMed ID10936177
'Brain microglia are a major source of inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), which have been implicated in the progression of neurodegenerative diseases. Recently, microglia were revealed to be highly responsive to ATP, which is released from nerve terminals, activated immune cells, or damaged cells. It is not ... More
Extracellular nucleotides regulate cellular functions of podocytes in culture.
AuthorsFischer KG, Saueressig U, Jacobshagen C, Wichelmann A, Pavenstädt H
JournalAm J Physiol Renal Physiol
PubMed ID11704558
'Extracellular nucleotides are assumed to be important regulators of glomerular functions. This study characterizes purinergic receptors in podocytes. The effects of purinergic agonists on electrophysiological properties and the intracellular free Ca(2+) concentration of differentiated podocytes were examined with the patch-clamp and fura 2 fluorescence techniques. mRNA expression of purinergic receptors ... More
P2X7 receptors in Müller glial cells from the human retina.
'ATP has been shown to be an important extracellular signaling molecule. There are two subgroups of receptors for ATP (and other purines and pyrimidines): the ionotropic P2X and the G-protein-coupled P2Y receptors. Different subtypes of these receptors have been identified by molecular biology, but little is known about their functional ... More
ATP-induced secretion in PC12 cells and photoaffinity labeling of receptors.
AuthorsRhoads AR, Parui R, Vu ND, Cadogan R, Wagner PD
JournalJ Neurochem
PubMed ID8228985
'Secretion of catecholamines by rat PC12 cells is strongly stimulated by extracellular ATP via a P2-type purinergic receptor. ATP-induced norepinephrine release was inhibited 80% when extracellular Ca2+ was absent. Only four nucleotides, ATP, ATP gamma S, benzoylbenzoyl ATP (BzATP), and 2-methylthio-ATP, gave substantial stimulation of norepinephrine release from PC12 cells. ... More
Functional expression and photoaffinity labeling of a cloned P2U purinergic receptor.
AuthorsErb L, Lustig KD, Sullivan DM, Turner JT, Weisman GA
JournalProc Natl Acad Sci U S A
PubMed ID8248130
'ATP and UTP can function as extracellular signaling molecules by activating plasma membrane receptors termed P2 purinergic receptors. In the present study a P2U receptor cDNA has been expressed in K-562 human leukemia cells, one of the few available mammalian cell lines that lacks an endogenous P2U receptor. In stably ... More
The human SH-SY5Y neuroblastoma cell-line expresses a functional P2X7 purinoceptor that modulates voltage-dependent Ca2+ channel function.
AuthorsLarsson KP, Hansen AJ, Dissing S
JournalJ Neurochem
PubMed ID12423239
'Fura-2 imaging of purinergic stimulation of non-differentiated neuronal human SH-SY5Y cells resulted in a rapid elevation in intracellular Ca2+ ([Ca2+]i) that was dependent on extracellular Ca2+. The rank order of agonists (200 micro m) was as follows: 2'',3''-O-(4-benzoyl-benzoyl)-ATP (BzATP) > ATP4- > ATP; whereas 2-(methylthio)-ATP, ADP, UTP and alpha,beta-methylene-ATP and ... More
UTP activates phospholipase C-Ca2+ system through a receptor different from the 53-kDa ATP receptor in PC12 cells.
AuthorsMajid MA, Okajima F, Kondo Y
JournalBiochem Biophys Res Commun
PubMed ID8363617
'Extracellular ATP (a purine nucleotide) and UTP (a pyrimidine nucleotide) both activated phospholipase C with a similar potency and efficacy; however, in contrast to ATP which induced a remarkable norepinephrine release, UTP-induced norepinephrine release was small in PC12 cells, a rat pheochromocytoma cell line. ATP, its derivatives (2-methylthioadenosine 5''-triphosphate (MeSATP) ... More
Identification and characterization of an endogenous P2X7 (P2Z) receptor in CHO-K1 cells.
AuthorsMichel AD, Chessell IP, Hibell AD, Simon J, Humphrey PP
JournalBr J Pharmacol
PubMed ID9863647
'CHO-K1 cells were examined for their cellular responses to the P2 receptor agonist, 2''- and 3''-O-(4-benzoylbenzoyl)-ATP (DbATP), and for the presence of mRNA for P2X receptors. Reverse transcriptase-polymerase chain reactions, using primers directed against the rat P2X subunits, detected the presence of P2X7 but not P2X1-P2X6 subunits. DbATP (EC50 approximately ... More
P2 purinoceptor of the globular substance in the otoconial membrane of the guinea pig inner ear.
AuthorsSuzuki H, Ikeda K, Furukawa M, Takasaka T
JournalAm J Physiol
PubMed ID9374638
'The biological characteristics of the globular substance, a precursor of otoconia, are unclear. In the present study, the ATP-induced internal free Ca2+ concentration ([Ca2+]i) changes of the globular substance and the ATP distribution in the vestibular organ were investigated using a Ca2+ indicator, fluo 3, and an adenine nucleotide-specific fluorochrome, ... More
ATP stimulation of P2X(7) receptors activates three different ionic conductances on cultured mouse Schwann cells.
AuthorsColomar A, Amédée T
JournalEur J Neurosci
PubMed ID11595031
'Extracellular ATP, by acting on P2 purinergic receptors, is a potent mediator of cell-to-cell communication both within and between the nervous and the immune systems. We show here by patch-clamp recording, fluorescent dye uptake and immunocytochemistry that, in cultured mouse Schwann cells, ATP activates a P2X(7) receptor associated with three ... More
Membrane phosphatidylserine distribution as a non-apoptotic signalling mechanism in lymphocytes.
AuthorsElliott JI, Surprenant A, Marelli-Berg FM, Cooper JC, Cassady-Cain RL, Wooding C, Linton K, Alexander DR, Higgins CF
JournalNat Cell Biol
PubMed ID16025105
'Phosphatidylserine (PS) exposure is normally associated with apoptosis and the removal of dying cells. We observed that PS is exposed constitutively at high levels on T lymphocytes that express low levels of the transmembrane tyrosine phosphatase CD45RB. CD45 was shown to be a negative regulator of PS translocation in response ... More
Pseudoapoptosis induced by brief activation of ATP-gated P2X7 receptors.
AuthorsMackenzie AB, Young MT, Adinolfi E, Surprenant A
JournalJ Biol Chem
PubMed ID15994333
'P2X7 receptors are ATP-gated ion channels primarily expressed on antigen-presenting immune cells where they play a role in the acute inflammatory response. These ion channels couple not only to influx of cations, including calcium, but also to rapid alterations in cell morphology (membrane blebbing, phosphatidylserine exposure, microvesicle shedding). These features ... More
Neuronal P2X7 receptors are targeted to presynaptic terminals in the central and peripheral nervous systems.
AuthorsDeuchars SA, Atkinson L, Brooke RE, Musa H, Milligan CJ, Batten TF, Buckley NJ, Parson SH, Deuchars J
JournalJ Neurosci
PubMed ID11549725
'The ionotropic ATP receptor subunits P2X(1-6) receptors play important roles in synaptic transmission, yet the P2X(7) receptor has been reported as absent from neurons in the normal adult brain. Here we use RT-PCR to demonstrate that transcripts for the P2X(7) receptor are present in extracts from the medulla oblongata, spinal ... More
The P2Z-purinoceptor of human lymphocytes: actions of nucleotide agonists and irreversible inhibition by oxidized ATP.
AuthorsWiley JS, Chen JR, Snook MB, Jamieson GP
JournalBr J Pharmacol
PubMed ID7921625
'1. Extracellular adenosine triphosphate (ATP) is known to open a receptor-operated ion channel (P2Z class) in human lymphocytes which conducts a range of cationic permeants. The activity of a range of different agonists and inhibitors towards the P2Z-purinoceptor was investigated by measuring the agonist-induced influx of Ba2+ into fura-2 loaded ... More
A calcium channel in human submandibular duct cell line, HSG cells, not regulated by P2U purinergic receptor-mediated intracellular calcium mobilization.
AuthorsKurihara K, Nakanishi N, Ueha T
JournalArch Oral Biol
PubMed ID9347117
'Signal transduction via P2 purinergic receptors was investigated in HSG cells, a continuous cell line originally derived from an irradiated human salivary gland. Ligand specificity for nucleotide receptors in HSG cells was investigated with various nucleotides and their analogues. Inositol 1,4,5-trisphosphate (IP3) production was significantly increased by ATP, UTP and ... More
Mouse microglial cells express a plasma membrane pore gated by extracellular ATP.
AuthorsFerrari D, Villalba M, Chiozzi P, Falzoni S, Ricciardi-Castagnoli P, Di Virgilio F
JournalJ Immunol
PubMed ID8568257
'We have investigated responses to extracellular ATP (ATPe) in the microglial cell lines N9 and N13 and in freshly isolated mouse microglial cells. Upon stimulation with this nucleotide, N9 and N13 cells underwent an increase in the cytoplasmic free Ca2+ concentration ([Ca2+]i), a sustained depolarization of the plasma membrane, and ... More
Effect of erythromycin on ATP-induced intracellular calcium response in A549 cells.
AuthorsZhao DM, Xue HH, Chida K, Suda T, Oki Y, Kanai M, Uchida C, Ichiyama A, Nakamura H
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID10749750
'ATP induced a biphasic increase in the intracellular Ca(2+)concentration ([Ca(2+)](i)), an initial spike, and a subsequent plateau in A549 cells. Erythromycin (EM) suppressed the ATP-induced [Ca(2+)](i) spike but only in the presence of extracellular calcium (Ca(2+)(o)). It was ineffective against ATP- and UTP-induced inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)] formation and UTP-induced [Ca(2+)](i) ... More
The nucleotide receptor P2X7 mediates actin reorganization and membrane blebbing in RAW 264.7 macrophages via p38 MAP kinase and Rho.
AuthorsPfeiffer ZA, Aga M, Prabhu U, Watters JJ, Hall DJ, Bertics PJ
JournalJ Leukoc Biol
PubMed ID15075366
'Extracellular nucleotides regulate macrophage function via P2X nucleotide receptors that form ligand-gated ion channels. In particular, P2X7 activation is characterized by pore formation, membrane blebbing, and cytokine release. P2X7 is also linked to mitogen-activated protein kinases (MAPK) and Rho-dependent pathways, which are known to affect cytoskeletal structure in other systems. ... More
P2X purinergic receptor channel expression and function in bovine aortic endothelium.
AuthorsRamirez AN, Kunze DL
JournalAm J Physiol Heart Circ Physiol
PubMed ID12003818
'We examined bovine aortic endothelial cells (BAECs) for the functional expression of P2X receptors, the ATP-gated cation channels. We identified the P2X subtypes present in BAECs using RT-PCR. mRNA was present for only three of seven family members: P2X4, P2X5, and P2X7. We then characterized agonist-activated currents in whole cell ... More
Growth inhibitory effects of ATP and its derivatives on human fibroblasts immortalized with 60Co-gamma rays.
AuthorsLi JW, Inoue Y, Miyazaki M, Pu H, Kondo A, Namba M
JournalInt J Mol Med
PubMed ID10601575
'In our previous study (Katayama B et al, Int J Mol Med 2: 603-606, 1998), cell growth inhibition caused by ATP added to cultures was found to be greater in immortalized human fibroblasts than in the normal human fibroblasts. Since it has been reported that ATP affects cells via P2-purinergic ... More
Adenosine triphosphate-induced shedding of CD23 and L-selectin (CD62L) from lymphocytes is mediated by the same receptor but different metalloproteases.
AuthorsGu B, Bendall LJ, Wiley JS
JournalBlood
PubMed ID9680363
'CD23 is a transmembrane protein expressed on the surface of B-lymphocytes that binds IgE, CD21, CD11b, and CD11c. High concentrations of soluble CD23 and L-selectin are found in the serum of patients with B-chronic lymphocytic leukemia (B-CLL). Because extracellular adenosine triphosphate (ATP) causes shedding of L-selectin via activation of P2Z/P2X7 ... More
Heterogeneity of ATP receptors in aortic endothelial cells. Involvement of P2y and P2u receptors in inositol phosphate response.
AuthorsMotte S, Pirotton S, Boeynaems JM
JournalCirc Res
PubMed ID8431980
'Extracellular ATP plays an important role in the regulation of prostacyclin and nitric oxide release from vascular endothelial cells. These cellular responses to ATP are generally attributed to the stimulation of the P2y subtype of P2 purinergic receptors. However, it has recently been suggested that two types of ATP receptors ... More
Extracellular ATP triggers IL-1 beta release by activating the purinergic P2Z receptor of human macrophages.
AuthorsFerrari D, Chiozzi P, Falzoni S, Dal Susino M, Melchiorri L, Baricordi OR, Di Virgilio F
JournalJ Immunol
PubMed ID9233643
'Extracellular ATP (ATPe) is known to cause release of processed IL-1 beta from LPS-treated macrophages and microglial cells. IL-1 beta release is fast and thought to be associated with cell death. We have reinvestigated this process to identify 1) the purinergic receptor involved; 2) the relationship to cell death; and ... More
Activation of presynaptic P2X7-like receptors depresses mossy fiber-CA3 synaptic transmission through p38 mitogen-activated protein kinase.
AuthorsArmstrong JN, Brust TB, Lewis RG, MacVicar BA
JournalJ Neurosci
PubMed ID12122056
'P2X(7) receptor subunits form homomeric ATP-gated, calcium-permeable cation channels. In this study, we used Western blots and immunocytochemistry to demonstrate that P2X(7) receptors are abundant on presynaptic terminals of mossy fiber synapses in the rat hippocampus. P2X(7)-immunoreactive protein was detected using a specific P2X(7) antibody in Western blots of protein ... More
Effects of ATP and its analogues on [Ca2+]i dynamics in the rabbit corneal epithelium.
AuthorsKimura K, Nishimura T, Satoh Y
JournalArch Histol Cytol
PubMed ID10399537
'Adenosine-5''-triphosphate (ATP) plays a pivotal role in various tissues as an extracellular transmitter. ATP released from nerve endings and/or damaged cells may elicit reactions in adjacent cells. To identify such reactions, we investigated the dynamics of the intracellular calcium ion concentrations ([Ca2+]i) in the rabbit corneal epithelium during ATP-stimulation. Intact ... More
Nucleotide receptors: an emerging family of regulatory molecules in blood cells.
AuthorsDi Virgilio F, Chiozzi P, Ferrari D, Falzoni S, Sanz JM, Morelli A, Torboli M, Bolognesi G, Baricordi OR
JournalBlood
PubMed ID11157473
'Nucleotides are emerging as an ubiquitous family of extracellular signaling molecules. It has been known for many years that adenosine diphosphate is a potent platelet aggregating factor, but it is now clear that virtually every circulating cell is responsive to nucleotides. Effects as different as proliferation or differentiation, chemotaxis, release ... More
P2X7 mediates superoxide production in primary microglia and is up-regulated in a transgenic mouse model of Alzheimer's disease.
AuthorsParvathenani LK, Tertyshnikova S, Greco CR, Roberts SB, Robertson B, Posmantur R
JournalJ Biol Chem
PubMed ID12551918
'Primary rat microglia stimulated with either ATP or 2''- and 3''-O-(4-benzoylbenzoyl)-ATP (BzATP) release copious amounts of superoxide (O(2)(-)*). ATP and BzATP stimulate O(2)(-)* production through purinergic receptors, primarily the P2X(7) receptor. O(2)(-)* is produced through the activation of the NADPH oxidase. Although both p42/44 MAPK and p38 MAPK were activated ... More
Extracellular ATP causes apoptosis and necrosis of cultured mesangial cells via P2Z/P2X7 receptors.
AuthorsSchulze-Lohoff E, Hugo C, Rost S, Arnold S, Gruber A, Brüne B, Sterzel RB
JournalAm J Physiol
PubMed ID9843914
'Mesangial cells undergo cell death both by apoptosis and necrosis during glomerular disease. Since nucleotides are released from injured and destroyed cells in the glomerulus, we examined whether extracellular ATP and its receptors may regulate cell death of cultured mesangial cells. Addition of extracellular ATP (300 microM to 5 mM) ... More
Nuclear accumulation of G-actin in isolated rat hepatocytes by adenine nucleotides.
AuthorsMeijerman I, Blom WM, de Bont HJ, Mulder GJ, Nagelkerke JF
JournalBiochem Biophys Res Commun
PubMed ID9398629
'Extracellular ATP induces bleb formation in isolated rat hepatocytes. We examined the effect of extracellular ATP on the actin cytoskeleton of these hepatocytes. Exposure to 100 microM ATP caused pronounced nuclear accumulation of G-actin. ADP, AMP, adenosine, and dibutyryl-cAMP induced the same effect. Adenosine deaminase could inhibit both ATP- and ... More
Serine-324 of myosin's heavy chain is photoaffinity-labeled by 3'(2')-O-(4-benzoylbenzoyl)adenosine triphosphate.
AuthorsMahmood R, Elzinga M, Yount RG
JournalBiochemistry
PubMed ID2502175
'A portion of the active site of rabbit skeletal myosin near the ribose ring of ATP can be labeled by the photoaffinity analogue 3''(2'')-O-(4-benzoylbenzoyl)adenosine triphosphate (Bz2ATP). The specificity of the photolabeling was assured by first trapping [14C]Bz2ATP at the active site by use of thiol cross-linking agents [Mahmood, R., Cremo, ... More
P2X agonist BzATP interferes with amplex-red-coupled fluorescence assays.
AuthorsLeón D, Marín-García P, Sánchez-Nogueiro J, de la O FO, García-Carmona F, Miras-Portugal MT,
JournalAnal Biochem
PubMed ID17562321
'This paper does not have an abstract.'
Rat astroglial P2Z (P2X7) receptors regulate intracellular calcium and purine release.
AuthorsBallerini P, Rathbone MP, Di Iorio P, Renzetti A, Giuliani P, D'Alimonte I, Trubiani O, Caciagli F, Ciccarelli R
JournalNeuroreport
PubMed ID8981418
'Treatment of rat astrocyte cultures with 2''- and 3''-O-(4-benzoylbenzoyl)-adenosine 5''-triphosphate (BzATP), a P2X7 agonist, but not with adenosine 5-[alpha, beta methylene] triphosphate (alpha, beta meATP), a P2X agonist, increased influx of extracellular Ca2+ and [Ca2+]i. Lucifer yellow, a small molecule which permeates P2X7 receptor-induced pores, entered BzATP-treated but not control ... More
Heightened ability of monocytes from sarcoidosis patients to form multi-nucleated giant cells in vitro by supernatants of concanavalin A-stimulated mononuclear cells.
AuthorsMizuno K, Okamoto H, Horio T
JournalClin Exp Immunol
PubMed ID11678912
'The main immunocompetent cells in sarcoidal lesions are epithelioid cells and multi-nucleated giant cells (MGC), both of which are derived from monocyte-macrophage lineage cells. To understand further the relevance of monocytes in sarcoidosis, we examined in vitro MGC formation using monocytes from sarcoidosis patients, patients with other granulomatous diseases (OGD) ... More
Bromoenol lactone enhances the permeabilization of rat submandibular acinar cells by P2X7 agonists.
AuthorsChaib N, Kabré E, Alzola E, Pochet S, Dehaye JP
JournalBr J Pharmacol
PubMed ID10683195
'The permeabilizing effect of P2X(7) agonists was tested in rat submandibular acinar cells using the uptake of ethidium bromide as an index. The uptake of ethidium bromide by acini incubated at 37 degrees C in the presence of 1 mM ATP increased with time and reached after 5 min about ... More
Human primary fibroblasts in vitro express a purinergic P2X7 receptor coupled to ion fluxes, microvesicle formation and IL-6 release.
AuthorsSolini A, Chiozzi P, Morelli A, Fellin R, Di Virgilio F
JournalJ Cell Sci
PubMed ID9885283
'We have investigated reponses to extracellular ATP in human fibroblasts obtained by skin biopsies. Our data show that these cells express a P2X7 purinergic receptor, as judged by (1) RT-PCR with specific primers, (2) reactivity with a specific anti-P2X7 antiserum, (3) activation by the selective P2X agonist benzoylbenzoylATP and (4) ... More
Molecular and functional characterization of human P2X(2) receptors.
AuthorsLynch KJ, Touma E, Niforatos W, Kage KL, Burgard EC, van Biesen T, Kowaluk EA, Jarvis MF
JournalMol Pharmacol
PubMed ID10570044
'P2X receptors are a family of ATP-gated ion channels. Four cDNAs with a high degree of homology to the rat P2X(2) receptor were isolated from human pituitary and pancreas RNA. Genomic sequence indicated that these cDNAs represent alternatively spliced messages. Northern analysis revealed high levels of human P2X(2) (hP2X(2)) message ... More
Confocal calcium imaging reveals an ionotropic P2 nucleotide receptor in the paranodal membrane of rat Schwann cells.
AuthorsGrafe P, Mayer C, Takigawa T, Kamleiter M, Sanchez-Brandelik R
JournalJ Physiol
PubMed ID10050005
'1. The paranodal Schwann cell region is of major importance for the function of a myelinated axon. In the present study we searched for a possible ionotropic effect of extracellular ATP in this Schwann cell compartment. 2. Whole-cell patch-clamp recordings from cultured rat Schwann cells revealed that ATP and 2''-3''-O-(4-benzoylbenzoyl)-adenosine ... More
Pharmacological characterization of recombinant human and rat P2X receptor subtypes.
AuthorsBianchi BR, Lynch KJ, Touma E, Niforatos W, Burgard EC, Alexander KM, Park HS, Yu H, Metzger R, Kowaluk E, Jarvis MF, van Biesen T
JournalEur J Pharmacol
PubMed ID10440098
'ATP functions as a fast neurotransmitter through the specific activation of a family of ligand-gated ion channels termed P2X receptors. In this report, six distinct recombinant P2X receptor subtypes were pharmacologically characterized in a heterologous expression system devoid of endogenous P2 receptor activity. cDNAs encoding four human P2X receptor subtypes ... More
Pharmacological characterization of ATP receptors in ampulla from frog semicircular canal.
AuthorsButlen D, Bernard C, Ferrary E
JournalAm J Physiol
PubMed ID9688986
'Phosphoinositidase C activities sensitive to purine and pyrimidine nucleotides have been identified earlier in ampulla from Rana ridibunda semicircular canal. The aim of this study was to characterize the pharmacological properties of other P2 receptors borne by this structure. A microassay was developed to measure the binding of [35S]adenosine 5'-O-(2-thiodiphosphate) ... More
Multiple purinergic receptors lead to intracellular calcium increases in cultured rat Sertoli cells.
AuthorsKo WH, Au CL, Yip CY
JournalLife Sci
PubMed ID12535719
'Our previous study has demonstrated the activation of calcium-dependent Cl(-) secretion through P2Y(2) receptors by extracellular nucleotides. To evaluate the contribution and involvement of other receptor subtypes to this physiological response, the purpose of this study was to investigate the regulation of intracellular calcium concentration ([Ca(2+)](i)) by different purinergic agonists ... More
Receptors for purines and pyrimidines.
AuthorsRalevic V, Burnstock G
JournalPharmacol Rev
PubMed ID9755289
Cloning and pharmacological characterization of the guinea pig P2X7 receptor orthologue.
AuthorsFonfria E, Clay WC, Levy DS, Goodwin JA, Roman S, Smith GD, Condreay JP, Michel AD,
JournalBr J Pharmacol
PubMed ID18037910
BACKGROUND AND PURPOSE: The human, rat, and mouse P2X(7) receptors have been previously characterized, and in this study we report the cloning and pharmacological properties of the guinea pig orthologue. EXPERIMENTAL APPROACH: A cDNA encoding for the guinea pig P2X(7) receptor was isolated from a guinea pig brain library. The ... More
Sequential shrinkage and swelling underlie P2X7-stimulated lymphocyte phosphatidylserine exposure and death.
AuthorsTaylor SR, Gonzalez-Begne M, Dewhurst S, Chimini G, Higgins CF, Melvin JE, Elliott JI,
JournalJ Immunol
PubMed ID18097031
Patterns of change in cell volume and plasma membrane phospholipid distribution during cell death are regarded as diagnostic means of distinguishing apoptosis from necrosis, the former being associated with cell shrinkage and early phosphatidylserine (PS) exposure, whereas necrosis is associated with cell swelling and consequent lysis. We demonstrate that cell ... More
Blockade of human P2X7 receptor function with a monoclonal antibody.
A monoclonal antibody (MoAb) specific for the human P2X7 receptor was generated in mice. As assessed by flow cytometry, the MoAb labeled human blood-derived macrophage cells natively expressing P2X7 receptors and cells transfected with human P2X7 but not other P2X receptor types. The MoAb was used to immunoprecipitate the human ... More
Pharmacology of cloned P2X receptors.
AuthorsNorth RA, Surprenant A
JournalAnnu Rev Pharmacol Toxicol
PubMed ID10836147
There are seven P2X receptor cDNAs currently known. Six homomeric (P2X1, P2X2, P2X3, P2X4, P2X5, P2X7) and three heteromeric (P2X2/P2X3, P2X4/P2X6, P2X1/P2X5) P2X receptor channels have been characterized in heterologous expression systems. Homomeric P2X1 and P2X3 receptors are readily distinguishable by their rapid desensitization, the agonist action of alpha beta ... More
Purines, a new class of agonists in salivary glands?
AuthorsDehaye JP, Moran A, Marino A
JournalArch Oral Biol
PubMed ID10414854
The response of rat submandibular glands to extracellular purines was tested. In crude cellular suspensions, ATP increased the [Ca2+]i mostly by promoting uptake of extracellular calcium. ATP caused the pHi to drop, a response blocked by chloride channel inhibitors. ATP also inhibited the basal and isoproterenol-stimulated activity of the Na+ ... More
P2Z purinoceptor-associated pores induced by extracellular ATP in macrophages and J774 cells.
AuthorsCoutinho-Silva R, Persechini PM
JournalAm J Physiol
PubMed ID9435482
Millimolar concentrations of extracellular ATP (ATPo) can induce the permeabilization of plasma membranes of macrophages and other bone marrow-derived cells to low-molecular-weight solutes, a phenomenon that is the hallmark of P2Z purinoceptors. However, patch-clamp and whole cell electrophysiological experiments have so far failed to demonstrate the existence of any ATPo-induced ... More
P2Z/P2X7 receptor-dependent apoptosis of dendritic cells.
AuthorsCoutinho-Silva R, Persechini PM, Bisaggio RD, Perfettini JL, Neto AC, Kanellopoulos JM, Motta-Ly I, Dautry-Varsat A, Ojcius DM
JournalAm J Physiol
PubMed ID10329963
Macrophages and thymocytes express P2Z/P2X7 nucleotide receptors that bind extracellular ATP. These receptors play a role in immune development and control of microbial infections, but their presence on dendritic cells has not been reported. We investigated whether extracellular ATP could trigger P2Z/P2X7 receptor-dependent apoptosis of dendritic cells. Apoptosis could be ... More
AuthorsShigemoto-Mogami Y, Koizumi S, Tsuda M, Ohsawa K, Kohsaka S, Inoue K
JournalJ Neurochem
PubMed ID11579142
Microglia play various important roles in the CNS via the synthesis of cytokines. The ATP-evoked production of interleukin-6 (IL-6) and its intracellular signals were examined using a mouse microglial cell line, MG-5. ATP, but not its metabolites, produced IL-6 in a concentration-dependent manner. Although ATP activated two mitogen-activated protein kinases, ... More
Characterization and localization of P2 receptors in rat submandibular gland acinar and duct cells.
AuthorsLee MG, Zeng W, Muallem S
JournalJ Biol Chem
PubMed ID9407074
[Ca2+]i and the Cl- current were measured in isolated submandibular gland acinar and duct cells to characterize and localize the purinergic receptors expressed in these cells. In both cell types 2'-3'-benzoylbenzoyl (Bz)-ATP and ATP increased [Ca2+]i mainly by activation of Ca2+ influx. UTP had only minimal effect on [Ca2+]i at ... More
Unique functional properties of a sensory neuronal P2X ATP-gated channel from zebrafish.
AuthorsBoué-Grabot E, Akimenko MA, Séguéla P
JournalJ Neurochem
PubMed ID10987841
We report here the structural and functional characterization of an ionotropic P2X ATP receptor from the lower vertebrate zebrafish (Danio rerio). The full-length cDNA encodes a 410-amino acid-long channel subunit zP2X(3), which shares only 54% identity with closest mammalian P2X subunits. When expressed in XENOPUS: oocytes in homomeric form, ATP-gated ... More
Differential sensitivity to nickel and SK&F96365 of second messenger-operated and receptor-operated calcium channels in rat submandibular ductal cells.
AuthorsChaïb N, Kabré E, Métioui M, Alzola E, Dantinne C, Marino A, Dehaye JP
JournalCell Calcium
PubMed ID9924631
The intracellular concentration of calcium ([Ca2+]i) of rat submandibular ductal cells was measured with the intracellular fluorescent dye Fura-2. Carbachol (100 microM) and ATP (1 mM) both increased the [Ca2+]i. The late response to ATP was blocked by 0.5 mM Ni2+. This concentration of Ni2+ also blocked the increase of ... More
Study of nonspecific cation channel coupled to P2z purinergic receptors using an acid load technique.
AuthorsLachish M, Alzola E, Chaib N, Métioui M, Grosfils K, Kabré E, Moran A, Marino A, Dehaye JP
JournalAm J Physiol
PubMed ID8997193
The intracellular pH (pHi) of rat submandibular cells was measured by 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). The cells recovered from ammonium (30 mM) prepulse to their resting pHi within 10 min. Ethylisopropylamiloride (EIPA), an inhibitor of the Na+/H+ exchanger, slows the rate of pHi recovery. ATP (1 mM), in the presence of EIPA, ... More
Differential assembly of rat purinergic P2X7 receptor in immune cells of the brain and periphery.
AuthorsKim M, Spelta V, Sim J, North RA, Surprenant A
JournalJ Biol Chem
PubMed ID11313357
ATP-gated P2X(7) purinoceptors are found in most immune cells of the periphery and the brain where their activation leads to multiple downstream events such as cell permeabilization, apoptosis, and/or cytokine release. P2X(7) receptors do not form heteromeric receptors with any of the other six P2X subunits, and it is not ... More
Mechanical strain-induced Ca(2+) waves are propagated via ATP release and purinergic receptor activation.
AuthorsSauer H, Hescheler J, Wartenberg M
JournalAm J Physiol Cell Physiol
PubMed ID10912995
Mechanical strain applied to prostate cancer cells induced an intracellular Ca(2+) (Ca(i)(2+)) wave spreading with a velocity of 15 microm/s. Ca(i)(2+) waves were not dependent on extracellular Ca(2+) and membrane potential because propagation was unaffected in high-K(+) and Ca(2+)-free solution. Waves did not depend on the cytoskeleton or gap junctions ... More
P2X receptors in cochlear Deiters' cells.
AuthorsChen C, Bobbin RP
JournalBr J Pharmacol
PubMed ID9641551
1. The ionotropic purinoceptors in isolated Deiters' cells of guinea-pig cochlea were characterized by use of the whole-cell variant of the patch-clamp technique. 2. Extracellular application of adenosine 5'-triphosphate (ATP) induced a dose-dependent inward current when the cells were voltage-clamped at -80 mV. The ATP-induced current showed desensitization and had ... More
The role of a purinergic P2z receptor in calcium-dependent cell killing of isolated rat hepatocytes by extracellular adenosine triphosphate.
AuthorsZoetewij JP, van de Water B, de Bont HJ, Nagelkerke JF
JournalHepatology
PubMed ID8666342
Extracellular adenosine triphosphate (ATP o) (0.4 mmol/L), a P2-purinergic receptor agonist, induces cytolysis in several cell types including isolated rat hepatocytes. In this study, we investigated the P2-receptor involved in ATP o-induced, Ca2+-dependent cytotoxicity in hepatocytes. Pretreatment of hepatocytes with oxidized ATP, a P2z-receptor antagonist, or complexation of ATP(4-) (the ... More
Histamine inhibits ATP-induced [Ca2+]i rise through the activation of protein kinase A in HL-60 cells.
AuthorsSong SK, Suh BC, Lee H, Kim KT
JournalEur J Pharmacol
PubMed ID9098697
We investigated the cross-talk between the histamine and ATP receptors in HL-60 human promyelocytes. While both histamine and extracellular ATP increase intracellular Ca2+ concentration ([Ca2+]i) we found that histamine treatment causes a decrease in the subsequent ATP-induced Ca2+ release from intracellular stores and Ca2+ influx from extracellular space. In addition, ... More
Non-genomic inhibition of human P2X7 purinoceptor by 17beta-oestradiol.
AuthorsCario-Toumaniantz C, Loirand G, Ferrier L, Pacaud P
JournalJ Physiol
PubMed ID9518723
1. Effects of oestrogen on the current evoked by ATP and benzoylbenzoyl ATP (BzATP) in CV-1 monkey kidney cells transformed by SV 40 (COS cells) expressing the human P2X7 (hP2X7) purinoceptor were studied using standard patch-clamp techniques. 2. 17beta-Oestradiol rapidly and reversibly inhibited the whole-cell hP2X7 receptor cation current. This ... More
Sustained calcium entry through P2X nucleotide receptor channels in human airway epithelial cells.
AuthorsZsembery A, Boyce AT, Liang L, Peti-Peterdi J, Bell PD, Schwiebert EM
JournalJ Biol Chem
PubMed ID12566439
Purinergic receptor stimulation has potential therapeutic effects for cystic fibrosis (CF). Thus, we explored roles for P2Y and P2X receptors in stably increasing [Ca(2+)](i) in human CF (IB3-1) and non-CF (16HBE14o(-)) airway epithelial cells. Cytosolic Ca(2+) was measured by fluorospectrometry using the fluorescent dye Fura-2/AM. Expression of P2X receptor (P2XR) ... More
The P2X7 purinergic receptor on bovine macrophages mediates mycobacterial death.
AuthorsSmith RA, Alvarez AJ, Estes DM
JournalVet Immunol Immunopathol
PubMed ID11292527
P2X7 is an ATP gated purinoceptor that has been linked to various immune responses. P2X7 appears to be expressed ubiquitously in the immune system and thus may be important as an effector pathway or play significant roles in cell activation/death. 2',3'-(4-Benzoyl)benzoyl ATP is the most potent agonist of this receptor ... More
The P2 purinergic receptors of human dendritic cells: identification and coupling to cytokine release.
AuthorsFerrari D, La Sala A, Chiozzi P, Morelli A, Falzoni S, Girolomoni G, Idzko M, Dichmann S, Norgauer J, Di Virgilio F
JournalFASEB J
PubMed ID11099464
We investigated the expression of purinoceptors in human dendritic cells, providing functional, pharmacological, and biochemical evidence that immature and mature cells express P2Y and P2X subtypes, coupled to increase in the intracellular Ca(2+), membrane depolarization, and secretion of inflammatory cytokines. The ATP-activated Ca(2+) change was biphasic, with a fast release ... More
ATP-stimulated Ca2+ influx and phospholipase D activities of a rat brain-derived type-2 astrocyte cell line, RBA-2, are mediated through P2X7 receptors.
AuthorsSun SH, Lin LB, Hung AC, Kuo JS
JournalJ Neurochem
PubMed ID10386986
This study characterizes and examines the P2 receptor-mediated signal transduction pathway of a rat brain-derived type 2 astrocyte cell line, RBA-2. ATP induced Ca2+ influx and activated phospholipase D (PLD). The ATP-stimulated Ca2+ influx was inhibited by pretreating cells with P2 receptor antagonist, pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS), in a concentration-dependent manner. ... More
Activity-dependent development of P2X7 current and Ca2+ entry in rabbit osteoclasts.
AuthorsNaemsch LN, Dixon SJ, Sims SM
JournalJ Biol Chem
PubMed ID11495918
Bone remodeling is regulated by local factors and modulated by mechanical stimuli. Mechanical stimulation can cause release of ATP, an agent that stimulates osteoclastic resorption at low concentrations and inhibits at high concentrations. We examined whether osteoclasts express P2X(7) receptors, which are activated by high concentrations of ATP and can ... More
Kinetics of cell lysis, dye uptake and permeability changes in cells expressing the rat P2X7 receptor.
AuthorsVirginio C, MacKenzie A, North RA, Surprenant A
JournalJ Physiol
PubMed ID10457053
1. Extracellular ATP acting on P2X7 receptors opens a channel permeable to small cations, creates an access pathway for the entry of larger molecular weight dyes, and causes cell death. We used whole-cell recording and fluorescence microscopy to measure the time courses of ionic currents, uptake of the propidium dye ... More
Pharmacological characterization of heterologously expressed ATP-gated cation channels (P2x purinoceptors).
AuthorsEvans RJ, Lewis C, Buell G, Valera S, North RA, Surprenant A
JournalMol Pharmacol
PubMed ID7544432
cDNAs encoding P2x purinoceptors from human bladder smooth muscle and from rat PC-12 cells were expressed in oocytes and human embryonic kidney 293 cells. Agonist potencies of 2-methylthio-ATP = 2-chloro-ATP = ATP > = 2'- and 3'-O-(4-benzoylbenzoyl)-ATP > or = adenosine-5'-O-(3-thio)-triphosphate > or = P1,P5-di(adenosine-5') pentaphosphate >> ADP prevailed for ... More
Expression of P2X(7) purinoceptors on human lymphocytes and monocytes: evidence for nonfunctional P2X(7) receptors.
Lymphocytes from normal subjects and patients with B-chronic lymphocytic leukemia (B-CLL) show functional responses to extracellular ATP characteristic of the P2X(7) receptor (previously termed P2Z). These responses include opening of a cation-selective channel/pore that allows entry of the fluorescent dye ethidium and activation of a membrane metalloprotease that sheds the ... More
Microfluorimetric analysis of a purinergic receptor (P2X7) in GH4C1 rat pituitary cells: effects of a bioactive substance produced by Pfiesteria piscicida.
Pfiesteria piscicida Steidinger & Burkholder is a toxic dinoflagellate that leads to fish and human toxicity. It produces a bioactive substance that leads to cytotoxicity of GH4C1 rat pituitary cells. Extracellular adenosine 5'-triphosphate (ATP) acting on P2X7 purinergic receptors induces the formation of a nonselective cation channel, causing elevation of ... More
Selective knock-down of P2X7 ATP receptor function by dominant-negative subunits.
AuthorsRaouf R, Chakfe Y, Blais D, Speelman A, Boué-Grabot E, Henderson D, Séguéla P
JournalMol Pharmacol
PubMed ID14978243
Among the family of P2X ATP-gated cation channels, the P2X7 receptor is a homomeric subtype highly expressed in immune cells of the monocyte-macrophage lineage. We report here that the WC167-168AA mutation in the ectodomain of P2X7 produced nonfunctional subunits with strong dominant-negative effect on wild-type P2X7 receptors (77% inhibition with ... More
Ca(2+)- and nitric oxide-dependent stimulation of cyclic GMP synthesis in neuronal cell line induced by P2-purinergic/pyrimidinergic receptor.
AuthorsReiser G
JournalJ Neurochem
PubMed ID7798951
The mechanism by which cyclic GMP synthesis is activated through a nucleotide receptor was studied in mouse neuroblastoma x rat glioma hybrid cells [108CC15 (NG 108-15)]. The transient increase in cyclic GMP level induced by ATP reached its maximum at 20 s and lasted for approximately 1 min. The maximal ... More
Serum constituents can affect 2'-& 3'-O-(4-benzoylbenzoyl)-ATP potency at P2X(7) receptors.
AuthorsMichel AD, Xing M, Humphrey PP
JournalBr J Pharmacol
PubMed ID11264244
1. 2'-& 3'-O-(4-benzoylbenzoyl)-ATP (BzATP) is the prototypic agonist for P2X(7) receptors. In this study we demonstrate that bovine serum albumin (BSA) can affect the potency of BzATP at P2X receptors. 2. BzATP potency (pEC(50)) to stimulate ethidium accumulation in cells expressing recombinant P2X7 receptors varied between 6.5 and 4, depending ... More
Identification of a P2X7 receptor in GH(4)C(1) rat pituitary cells: a potential target for a bioactive substance produced by Pfiesteria piscicida.
We examined the pharmacologic activity of a putative toxin (pPfTx) produced by Pfiesteria piscicida by characterizing the signaling pathways that induce the c-fos luciferase construct in GH(4)C(1) rat pituitary cells. Adenosine-5'-triphosphate (ATP) was determined to increase and, at higher concentrations, decrease luciferase activity in GH(4)C(1) rat pituitary cells that stably ... More
Apparent species differences in the kinetic properties of P2X(7) receptors.
AuthorsHibell AD, Kidd EJ, Chessell IP, Humphrey PP, Michel AD
JournalBr J Pharmacol
PubMed ID10781013
1. Apparent species differences in the responses of recombinant P2X(7) receptors to repeated application of 2'- and 3'-O-(4-benzoylbenzoyl)-ATP (BzATP) have been investigated. 2. Repeated application of 100 microM BzATP resulted in a progressive increase in current magnitude (current growth) at mouse and human, but not rat P2X(7) receptors. 3. Current ... More
Species- and agonist-dependent differences in the deactivation-kinetics of P2X7 receptors.
AuthorsHibell AD, Thompson KM, Simon J, Xing M, Humphrey PP, Michel AD
JournalNaunyn Schmiedebergs Arch Pharmacol
PubMed ID11414659
In this study we have expressed recombinant P2X7 receptors in HEK293 cells and examined the reasons for the species- and agonist-dependent differences in the time taken for the closure of the P2X7 receptor ion-channels after agonist removal. Channel closure times, measured in electrophysiological studies or by measuring cellular permeability to ... More
Pharmacological characterization of ATP- and LPS-induced IL-1beta release in human monocytes.
AuthorsGrahames CB, Michel AD, Chessell IP, Humphrey PP
JournalBr J Pharmacol
PubMed ID10482924
1. We have utilized the human monocytic cell line, THP-1, and freshly isolated adherent human monocytes with the compounds pyridoxalphosphate-6-azophenyl-2',4'-disuphonic acid (PPADS), oxidized ATP, and 1-(N, O-bis[5-isoquinolinesufonyll]-N-methyl-L-tyrosyl)-4-phenylpiper azi ne (KN-62) to pharmacologically characterize the P2 receptor involved in ATP-induced release of interleukin 1beta (IL-1beta). We have also investigated the ... More
Pharmacological profile of a novel cyclic AMP-linked P2 receptor on undifferentiated HL-60 leukemia cells.
AuthorsConigrave AD, Lee JY, van der Weyden L, Jiang L, Ward P, Tasevski V, Luttrell BM, Morris MB
JournalBr J Pharmacol
PubMed ID9723974
1. Extracellular ATP (EC50=146+/-57 microM) and various ATP analogues activated cyclic AMP production in undifferentiated HL-60 cells. 2. The order of agonist potency was: ATPgammaS (adenosine 5'-O-[3-thiotriphosphate]) > or = BzATP (2'&3'O-(4-benzoylbenzoyl)-adenosine-5'-triphosphate) > or = dATP > ATP. The following agonists (in order of effectiveness at 1 mM) were all ... More
ATP released by LPS increases nitric oxide production in raw 264.7 macrophage cell line via P2Z/P2X7 receptors.
AuthorsSperlágh B, Haskó G, Németh Z, Vizi ES
JournalNeurochem Int
PubMed ID9759915
P2Z/P2X7 receptor is a particular type of purinoceptor, which is selectively expressed on the surface of immune cells in neuronal and non-neuronal tissues. Despite intensive research on its involvement in the immune response, the exact mechanism whereby it affects intercellular signaling is far from clear yet. In this study, the ... More
Mechanistic studies of reaction coupling in Glu-tRNAGln amidotransferase.
AuthorsHoriuchi KY, Harpel MR, Shen L, Luo Y, Rogers KC, Copeland RA
JournalBiochemistry
PubMed ID11371208
Organisms lacking Gln-tRNA synthetase produce Gln-tRNA(Gln) from misacylated Glu-tRNA(Gln) through the transamidation activity of Glu-tRNA(Gln) amidotransferase (Glu-AdT). Glu-AdT hydrolyzes Gln to Glu and NH(3), using the latter product to transamidate Glu-tRNA(Gln) in concert with ATP hydrolysis. In the absence of the amido acceptor, Glu-tRNA(Gln), the enzyme has basal glutaminase activity ... More
An ATP-gated cation channel with some P2Z-like characteristics in gastric smooth muscle cells of toad.
AuthorsUgur M, Drummond RM, Zou H, Sheng P, Singer JJ, Walsh JV
JournalJ Physiol
PubMed ID9032690
1. Whole-cell and single-channel currents elicited by extracellular ATP were studied in freshly dissociated smooth muscle cells from the stomach of the toad Bufo marinus using standard patch clamp and microfluorimetric techniques. 2. This ATP-gated cation channel shares a number of pharmacological and functional properties with native rat myometrium receptors, ... More
ATP, a partial agonist for the P2Z receptor of human lymphocytes.
AuthorsGargett CE, Cornish JE, Wiley JS
JournalBr J Pharmacol
PubMed ID9384508
1. Although extracellular adenosine 5'-triphosphate (ATP) is the natural ligand for the P2Z receptor of human lymphocytes it is less potent than 3'-O-(4-benzoylbenzoyl)-ATP (BzATP) in opening the associated ion channel, which conducts a range of permeants including Ba2+ and ethidium+. We have quantified the influx of ethidium+ into lymphocytes produced ... More
Two different ionotropic receptors are activated by ATP in rat microglia.
AuthorsVisentin S, Renzi M, Frank C, Greco A, Levi G
JournalJ Physiol
PubMed ID10457086
1. Our aim was to assess whether ATP-induced inward currents in microglia are due to a single or more than one purinergic receptor. The ATP dose-response curve showed two components, whose presence might be due to the activation of high and low affinity receptors. 2. The P2Z/P2X7 specific receptor agonist ... More
Expression of a P2X7 receptor by a subpopulation of human osteoblasts.
AuthorsGartland A, Hipskind RA, Gallagher JA, Bowler WB
JournalJ Bone Miner Res
PubMed ID11341329
There is now conclusive evidence that extracellular nucleotides acting via cell surface P2 receptors are important local modulators of bone cell function. Multiple subtypes of P2 receptors have been localized to bone, where their activation modulates multiple processes including osteoblast proliferation, osteoblast-mediated bone formation, and osteoclast formation and resorptive capacity. ... More
Effects of antagonists at the human recombinant P2X7 receptor.
AuthorsChessell IP, Michel AD, Humphrey PP
JournalBr J Pharmacol
PubMed ID9720806
1. We have used whole-cell patch clamping methods to examine the properties of the recombinant human P2X7 (P2Z) receptor stably expressed in HEK-293 cells. 2. In an extracellular solution with lowered concentrations of divalent cations (zero Mg2+ and 0.5 mM Ca2+), both ATP and the nucleotide analogue, 2'- and 3'-O-(4-benzoylbenzoyl)-adenosine ... More
Evidence that multiple P2X purinoceptors are functionally expressed in rat supraoptic neurones.
AuthorsShibuya I, Tanaka K, Hattori Y, Uezono Y, Harayama N, Noguchi J, Ueta Y, Izumi F, Yamashita H
JournalJ Physiol
PubMed ID9852319
1. The expression, distribution and function of P2X purinoceptors in the supraoptic nucleus (SON) were investigated by reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, and Ca2+-imaging and whole-cell patch-clamp techniques, respectively. 2. RT-PCR analysis of all seven known P2X receptor mRNAs in circular punches of the SON revealed that ... More
Identification of competitive antagonists of the P2Y1 receptor.
Although P2 receptors mediate a myriad of physiological effects of extracellular adenine nucleotides, study of this broad class of receptors has been compromised by a lack of P2 receptor-selective antagonist molecules. The adenine nucleotide-promoted inositol lipid hydrolysis response of turkey erythrocyte membranes, which has been used extensively as a model ... More
Properties of the pore-forming P2X7 purinoceptor in mouse NTW8 microglial cells.
AuthorsChessell IP, Michel AD, Humphrey PP
JournalBr J Pharmacol
PubMed ID9257924
1. We have used whole-cell patch clamping methods to study and characterize the cytolytic P2X7 (P2Z) receptor in the NTW8 mouse microglial cell line. 2. At room temperature, in an extracellular solution containing 2 mM Ca2+ and 1 mM Mg2+, 2'- and 3'-O-(4-benzoylbenzoyl)-adenosine-5'-triphosphate (Bz-ATP; 300 microM), or ATP (3 mM), ... More
2'-Deoxy-3'-O-(4-benzoylbenzoyl)- and 3'(2')-O-(4-benzoylbenzoyl)-1,N6-ethenoadenosine 5'-diphosphate, fluorescent photoaffinity analogues of adenosine 5'-diphosphate. Synthesis, characterization, and interaction with myosin subfragment 1.
AuthorsCremo CR, Yount RG
JournalBiochemistry
PubMed ID3427092
Two new fluorescent nucleotide photoaffinity labels, 3'(2')-O-(4-benzoylbenzoyl)-1,N6-ethenoadenosine 5'-diphosphate (Bz2 epsilon ADP) and 2'-deoxy-3'-O-(4-benzoylbenzoyl)-1,N6-ethenoadenosine 5'-diphosphate [3'(Bz2)2'd epsilon ADP], have been synthesized and used as probes of the ATP binding site of myosin subfragment 1 (SF1). These analogues are stably trapped by the bifunctional thiol cross-linker N,N'-p-phenylenedimaleimide (pPDM) at the active site ... More
Analysis of nucleotide binding by a vacuolar proton-translocating adenosine triphosphatase.
AuthorsWebster LC, Apps DK
JournalEur J Biochem
PubMed ID8797849
The vacuolar-type proton-translocatine adenosine triphosphatase from bovine adrenal secretory granules (chromaffin granules) was purified and reconstituted into proteoliposomes. The binding of nucleotides to the enzyme was studied by quantifying their effects on the rate of inactivation by N-ethylmaleimide (MalNEt) of ATP-dependent proton translocation, and by direct measurement of the binding ... More
Extended pharmacological profiles of rat P2Y2 and rat P2Y4 receptors and their sensitivity to extracellular H+ and Zn2+ ions.
AuthorsWildman SS, Unwin RJ, King BF
JournalBr J Pharmacol
PubMed ID14581177
1. Two molecularly distinct rat P2Y receptors activated equally by adenosine-5'-triphosphate (ATP) and uridine-5'-triphosphate (UTP) (rP2Y2 and rP2Y4 receptors) were expressed in Xenopus oocytes and studied extensively to find ways to pharmacologically distinguish one from the other. 2. Both P2Y subtypes were activated fully by a number of nucleotides. Tested ... More
Identification of P2X7 (P2Z) receptor in N18TG-2 cells and NG108-15 cells.
AuthorsKaiho H, Matsuoka I, Kimura J, Nakanishi H
JournalJ Neurochem
PubMed ID9489714
ATP activates a nonselective cation current by stimulating the P2Z receptor in NG108-15 cells--a hybrid cell line of the mouse neuroblastoma N18TG-2 cells and the rat glioma C6Bu-1 cells. Recently, the P2X7 receptor was cloned from the rat brain and was found to have electrophysiological properties similar to those of ... More
Pharmacological and biophysical properties of the human P2X5 receptor.
AuthorsBo X, Jiang LH, Wilson HL, Kim M, Burnstock G, Surprenant A, North RA
JournalMol Pharmacol
PubMed ID12761352
We constructed a full-length human P2X5 purinoceptor cDNA by incorporating a sequence corresponding to exon 10, which is missing in cDNAs cloned previously from human tissues. We studied the functional properties by patch-clamp recording and fluorescence imaging after expression in human embryonic kidney 293 cells. ATP (1-100 microM; half-maximal current ... More
P2X7 receptor activation-induced contraction and lysis in human saphenous vein smooth muscle.
AuthorsCario-Toumaniantz C, Loirand G, Ladoux A, Pacaud P
JournalCirc Res
PubMed ID9686759
In cutaneous veins where purinergic neurotransmission is more prominent compared with in deep vessels, physiological and pathological roles of nerve-released ATP have been described. Neuronally released ATP has been reported to act through activation of unidentified ionotropic P2X receptor(s). This study analyzed P2X receptor subtypes expressed in human saphenous vein ... More
Induction of proliferation and apoptotic cell death via P2Y and P2X receptors, respectively, in rat glomerular mesangial cells.
AuthorsHarada H, Chan CM, Loesch A, Unwin R, Burnstock G
JournalKidney Int
PubMed ID10720948
BACKGROUND: Cell surface receptors for adenosine 5'-triphosphate (ATP; P2 receptors) have been subdivided into two families: ligand-gated ion channels (P2X1-7) and G-protein-coupled (P2Y1-8) receptors. We investigated the potential role of P2 receptors on rat glomerular mesangial cells. METHODS: To investigate cell proliferation, DNA synthesis was assayed by measuring [3H]thymidine incorporation ... More
P2X7 nucleotide receptors mediate blebbing in osteoblasts through a pathway involving lysophosphatidic acid.
AuthorsPanupinthu N, Zhao L, Possmayer F, Ke HZ, Sims SM, Dixon SJ
JournalJ Biol Chem
PubMed ID17135244
Extracellular nucleotides, released in response to mechanical or inflammatory stimuli, signal through P2 receptors in many cell types, including osteoblasts. P2X7 receptors are ATP-gated cation channels that can induce formation of large membrane pores. Disruption of the gene encoding the P2X7 receptor leads to decreased periosteal bone formation and insensitivity ... More