Phosphate-specific fluorescence labeling with BO-IMI: reaction details.
AuthorsWang P, Giese RW
JournalJ Chromatogr A
PubMed ID9677715
'Previously we reported than BO-IMI, a reagent which contains a BODIPY fluorophore linked to an imidazole group, can be used to covalently label a phosphomonoester in a single step under aqueous conditions [P. Wang, R.W. Giese, Anal. Chem. 65 (1993) 3518]. The reaction was conducted in the presence of a ... More
H1 and H2 histamine receptors are absent on Langerhans cells and present on dermal dendritic cells.
'Human monocyte-derived dendritic cells (MoDC) have both histamine H1 and H2 receptors and can induce CD86 expression by histamine. Nevertheless, it has not been reported whether human epidermal Langerhans cells (LC) have histamine receptors or not. In this study, using RT-PCR, we investigated the expression of H1 and H2 receptor ... More
Histamine H4 receptor stimulation suppresses IL-12p70 production and mediates chemotaxis in human monocyte-derived dendritic cells.
AuthorsGutzmer R, Diestel C, Mommert S, Köther B, Stark H, Wittmann M, Werfel T,
JournalJ Immunol
PubMed ID15843518
'There is increasing evidence that histamine as an important mediator of immediate type allergic reactions also effects professional APCs. Recent reports showed effects of histamine on human monocyte-derived dendritic cells (MoDC) mediated primarily via histamine H1 receptors (H1R) and H2R. We show here that MoDC also express H3R and H4R ... More
Preparation of an IMI dye (imidazole functional group) containing a 4-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole fluorophore for labeling of phosphomonoesters.
AuthorsLan ZH, Qian X, Giese RW
JournalJ Chromatogr A
PubMed ID10070766
'We are studying dye-imidazole conjugates ("IMI dyes") as reagents for labeling phosphomonoesters such as nucleotides. Previously we have employed a BODIPY dye in our IMI reagents, and analyzed the labeled products by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) involving an argon ion laser. (The BODIPY fluorophore is a 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene). ... More
Receptor-independent, vacuolar ATPase-mediated cellular uptake of histamine receptor-1 ligands: possible origin of pharmacological distortions and side effects.
'The aims of this study were to investigate whether several histamine receptor agonists and antagonists are subjected to receptor-independent ion trapping into acidic organelles, and whether this sequestration influences their pharmacological or toxicological properties. Vacuolar (V)-ATPase-dependent intracellular sequestration of agonists was recognized as morphological alterations (large fluid-filled vacuoles for betahistine ... More