Separation of phospholipids in microfluidic chip device: application to high-throughput screening assays for lipid-modifying enzymes.
AuthorsLin S, Fischl AS, Bi X, Parce W
JournalAnal Biochem
PubMed ID12633607
Phospholipid molecules such as ceramide and phosphoinositides play crucial roles in signal transduction pathways. Lipid-modifying enzymes including sphingomyelinase and phosphoinositide kinases regulate the generation and degradation of these lipid-signaling molecules and are important therapeutic targets in drug discovery. We now report a sensitive and convenient method to separate these lipids ... More