Activation of proteinase-activated receptor-2 in mesothelial cells induces pleural inflammation.
AuthorsLee YC, Knight DA, Lane KB, Cheng DS, Koay MA, Teixeira LR, Nesbitt JC, Chambers RC, Thompson PJ, Light RW
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID15591415
'Pleural inflammation underlies many pleural diseases, but its pathogenesis remains unclear. Proteinase-activated receptor-2 (PAR(2)) is a novel seven-transmembrane receptor with immunoregulatory roles. We hypothesized that PAR(2) is present on mesothelial cells and can induce pleural inflammation. PAR(2) was detected by immunohistochemistry in all (19 parietal and 11 visceral) human pleural ... More
Scavenger receptor classes A and B. Their roles in atherogenesis and the metabolism of modified LDL and HDL.
AuthorsVan Berkel TJ, Van Eck M, Herijgers N, Fluiter K, Nion S
JournalAnn N Y Acad Sci
PubMed ID10865831
'Scavenger-receptor class A has been held responsible for the clearance of modified LDL from the blood circulation. However, in mice deficient in scavenger-receptor class A, the decay in vivo of acetylated LDL (t1/2 < 2 min), as well as tissue distribution and liver uptake (at 5 min 77.4 +/- 4.6% ... More
Role of phospholipid transfer protein in high-density lipoprotein metabolism: insights from studies in transgenic mice.
AuthorsVan Tol A, Jauhiainen M, De Crom R, Ehnholm C
JournalInt J Tissue React
PubMed ID10937357
The role of oxidized low-density lipoproteins in the pathogenesis of atherosclerosis.
AuthorsParthasarathy S, Steinberg D, Witztum JL
JournalAnnu Rev Med
PubMed ID1580586
Hypercholesterolemia is a major risk for atherogenesis. Recent evidence suggests that oxidative modification of the major cholesterol-carrying lipoprotein, low-density lipoprotein (LDL), renders it more atherogenic. Not only does oxidized LDL (Ox-LDL) have enhanced uptake by macrophages, which contributes directly to foam cell formation, it may also adversely affect many other ... More
Scavenger receptors that recognize advanced glycation end products.
AuthorsMiyazaki A, Nakayama H, Horiuchi S
JournalTrends Cardiovasc Med
PubMed ID12242049
Scavenger receptors recognize modified low-density lipoproteins (LDLs) such as acetylated LDL and oxidized LDL. Advanced glycation end products (AGE), which are generated through long-term exposure of proteins to glucose, also behave as active ligands for some scavenger receptors, including class A scavenger receptor (SR-A) and class B scavenger receptors such ... More
Competitive fluorescence polarization assays for the detection of phosphoinositide kinase and phosphatase activity.
AuthorsDrees BE, Weipert A, Hudson H, Ferguson CG, Chakravarty L, Prestwich GD
JournalComb Chem High Throughput Screen
PubMed ID12769675
We describe the development and implementation of competitive fluorescence polarization (FP) based assays for determining activity of phosphoinositide 3-kinase (PI 3-K) and the type-II SH2-domain-containing inositol 5-phosphatase (SHIP2). These assays are based on the interaction of specific phosphoinositide binding proteins with fluorophore-labeled phosphoinositide and inositol phosphate tracers. Enzyme reaction products ... More
Roles of lectin-like oxidized LDL receptor-1 and its soluble forms in atherogenesis.
AuthorsKume N, Kita T
JournalCurr Opin Lipidol
PubMed ID11507327
Lectin-like oxidized LDL receptor (LOX)-1 is a type II membrane protein that belongs to the C-type lectin family of molecules, which can act as a cell-surface endocytosis receptor for atherogenic oxidized LDL. LOX-1 can support binding, internalization and proteolytic degradation of oxidized LDL, but not of significant amounts of acetylated ... More
Endothelium-derived vasoactive mediators and renal glomerular function.
AuthorsBallermann BJ, Marsden PA
JournalClin Invest Med
PubMed ID1794204
Glomerular endothelial cells are located in extremely close proximity to glomerular mesangial cells, without intervening basement membrane. This close apposition of the two cell types suggest that interactions between the cells should readily occur. Given that endothelial cells are known to produce mediators which regulate the tone of underlying vascular ... More