Raft composition at physiological temperature and pH in the absence of detergents.
AuthorsAyuyan AG, Cohen FS,
JournalBiophys J
PubMed ID17993486
'Biological rafts were identified and isolated at 37 degrees C and neutral pH. The strategy for isolating rafts utilized membrane tension to generate large domains. For lipid compositions that led only to microscropically unresolvable rafts in lipid bilayers, membrane tension led to the appearance of large, observable rafts. The large ... More
Ouabain binding site in a functioning Na+/K+ ATPase.
AuthorsSandtner W, Egwolf B, Khalili-Araghi F, Sánchez-Rodríguez JE, Roux B, Bezanilla F, Holmgren M,
JournalJ Biol Chem
PubMed ID21911500
The Na(+)/K(+) ATPase is an almost ubiquitous integral membrane protein within the animal kingdom. It is also the selective target for cardiotonic derivatives, widely prescribed inhibitors for patients with heart failure. Functional studies revealed that ouabain-sensitive residues distributed widely throughout the primary sequence of the protein. Recently, structural work has ... More