The oesophageal string test: a novel, minimally invasive method measures mucosal inflammation in eosinophilic oesophagitis.
AuthorsFuruta GT, Kagalwalla AF, Lee JJ, Alumkal P, Maybruck BT, Fillon S, Masterson JC, Ochkur S, Protheroe C, Moore W, Pan Z, Amsden K, Robinson Z, Capocelli K, Mukkada V, Atkins D, Fleischer D, Hosford L, Kwatia MA, Schroeder S, Kelly C, Lovell M, Melin-Aldana H, Ackerman SJ,
JournalGut
PubMed ID22895393
'Eosinophil predominant inflammation characterises histological features of eosinophilic oesophagitis (EoE). Endoscopy with biopsy is currently the only method to assess oesophageal mucosal inflammation in EoE. We hypothesised that measurements of luminal eosinophil-derived proteins would correlate with oesophageal mucosal inflammation in children with EoE. The Enterotest diagnostic device was used to ... More
Rapid, absolute, and simultaneous quantification of specific pathogenic strain and total bacterial cells using an ultrasensitive dual-color flow cytometer.
AuthorsYang L, Wu L, Zhu S, Long Y, Hang W, Yan X,
JournalAnal Chem
PubMed ID20039721
'This paper describes a rapid and sensitive strategy for the absolute and simultaneous quantification of specific pathogenic strain and total bacterial cells in a mixture. A laboratory-built compact, high-sensitivity, dual channel flow cytometer (HSDCFCM) was modified to enable dual fluorescence detection. A bacterial cell mixture comprising heat-killed pathogenic Escherichia coli ... More
Combining phage and staphylococcal surface display for generation of ErbB3-specific Affibody molecules.
AuthorsKronqvist N, Malm M, Göstring L, Gunneriusson E, Nilsson M, Höidén Guthenberg I, Gedda L, Frejd FY, Ståhl S, Löfblom J,
JournalProtein Eng Des Sel
PubMed ID21177282
'Emerging evidence suggests that the catalytically inactive ErbB3 (HER3) protein plays a fundamental role in normal tyrosine kinase receptor signaling as well as in aberrant functioning of these signaling pathways, resulting in several forms of human cancers. ErbB3 has recently also been implicated in resistance to ErbB2-targeting therapies. Here we ... More
Antibody colocalization microarray: a scalable technology for multiplex protein analysis in complex samples.
AuthorsPla-Roca M, Leulmi RF, Tourekhanova S, Bergeron S, Laforte V, Moreau E, Gosline SJ, Bertos N, Hallett M, Park M, Juncker D,
JournalMol Cell Proteomics
PubMed ID22171321
'DNA microarrays were rapidly scaled up from 256 to 6.5 million targets, and although antibody microarrays were proposed earlier, sensitive multiplex sandwich assays have only been scaled up to a few tens of targets. Cross-reactivity, arising because detection antibodies are mixed, is a known weakness of multiplex sandwich assays that ... More
Molecular targeting of intracellular compartments specifically in cancer cells.
AuthorsPandya H, Gibo DM, Debinski W,
JournalGenes Cancer
PubMed ID20740056
We have implemented a strategy in which a genetically engineered, single-chain protein specifically recognizes cancer cells and is trafficked to a targeted subcellular compartment, such as the nucleus. The recombinant protein termed IL-13.E13K-D2-NLS has a triple functional property: (1) it binds a cancer-associated receptor, interleukin 13 receptor alpha 2 (IL-13Ra2), ... More
Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
AuthorsYao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Boström KI,
JournalBlood
PubMed ID22474252
The importance of morphogenetic proteins (BMPs) and their antagonists in vascular development is increasingly being recognized. BMP-4 is essential for angiogenesis and is antagonized by matrix Gla protein (MGP) and crossveinless 2 (CV2), both induced by the activin receptor like-kinase 1 (ALK1) when stimulated by BMP-9. In this study, however, ... More