Regional crypt function in rat large intestine in relation to fluid absorption and growth of the pericryptal sheath.
AuthorsNaftalin RJ, Pedley KC
JournalJ Physiol
PubMed ID9831728
'1. Confocal microscopic studies of rat colonic mucosa showed that the pericryptal sheath surrounding distal colonic crypts is an effective barrier both to dextran and NaCl movement, whereas no such structure surrounds the caecal crypts. 2. The distal colonic pericryptal barrier was functionally demonstrated by accumulation of Sodium Green within ... More
Paxillin isoforms in mouse. Lack of the gamma isoform and developmentally specific beta isoform expression.
AuthorsMazaki Y, Uchida H, Hino O, Hashimoto S, Sabe H
JournalJ Biol Chem
PubMed ID9712867
'Paxillin, a focal adhesion protein, exists as multiple isoforms in humans (alpha, beta, and gamma). To understand more about the physiological role of each isoform, we have employed the mouse system. We found that although the alpha and beta isoforms are present in the mouse, the gamma isoform is not. ... More
Phallotoxin and actin binding assay by fluorescence enhancement.
AuthorsHuang ZJ, Haugland RP, You WM, Haugland RP
JournalAnal Biochem
PubMed ID1595896
The fluorescence of five fluorophores conjugated to phallotoxins was found to be specifically enhanced upon binding to F-actin in a polymerizing buffer. Rhodamine phalloidin had the greatest fluorescence enhancement of ninefold. The fluorescence titration of rhodamine phalloidin by actin was shown to be consistent with stoichiometric binding. The fluorescence enhancement ... More
Changes in actin organization during the cytotoxic process.
AuthorsRadosevic K, van Leeuwen MT, Segers-Nolten IM, Figdor CG, de Grooth BG, Greve J
JournalCytometry
PubMed ID8026222
Changes in organization of F-actin during the cytotoxic process between NK and K562 cells have been observed and studied using confocal laser scanning microscopy and quantitative fluorescence microscopy. An increase in F-actin content and orientation of F-actin towards the target cell have been observed in conjugated NK cells. The increase ... More
TGF-beta3, but not TGF-beta1, protects keratinocytes against 12-O-tetradecanoylphorbol-13-acetate-induced cell death in vitro and in vivo.
AuthorsLi J, Foitzik K, Calautti E, Baden H, Doetschman T, Dotto GP
JournalJ Biol Chem
PubMed ID9933619
We have examined the role that individual TGF-beta isoforms, and in particular TGF-beta3, play in control of epidermal homeostasis. Mice with a knockout mutation of the TGF-beta3 gene die a few hours after birth. A full-thickness skin grafting approach was used to investigate the postnatal development and homeostatic control of ... More
Quantitative image analysis of F-actin in endothelial cells.
AuthorsEhringer WD, Yamany S, Steier K, Farag A, Roisen FJ, Dozier A, Miller FN
JournalMicrocirculation
PubMed ID10654280
OBJECTIVE: Filamentous actin (F-actin) plays a central role in maintaining endothelial barrier function. Thrombin and histamine, two inflammatory mediators that increase endothelial permeability, can alter F-actin production and distribution. In this study, we use a newly developed image analysis technique to show that these two inflammatory mediators differentially alter F-actin ... More