Characterization of novel fluorescent ligands with high affinity for D1 and D2 dopaminergic receptors.
AuthorsMonsma FJ, Barton AC, Kang HC, Brassard DL, Haugland RP, Sibley DR
JournalJ Neurochem
PubMed ID2523470
'We have synthesized and characterized a series of novel fluorescently labeled ligands with high affinity and specificity for D1 and D2 dopamine receptors. D1-selective probes were synthesized using (R,S)-5-(4''-aminophenyl)-8-chloro-2,3,4,5-tetrahydro-3-methyl- [1H]-3-benzazepin-7-ol, the 4''-amino derivative of the high-affinity, D1-selective antagonist SCH-23390, whereas D2-selective probes were synthesized using the high-affinity, D2-selective antagonist N-(p-aminophenethyl)spiperone ... More
Localization of D1 dopamine receptors on live cultured striatal neurons by quantitative fluorescence microscopy.
AuthorsHoyt KR, Reynolds IJ
JournalBrain Res
PubMed ID8883850
Single neurons in culture express a heterogeneity of neurotransmitter receptor subtypes. The study of the effects of neurotransmitters on neuronal function is complicated by this heterogeneity. It would therefore be useful to be able to identify live neurons that express the receptors of interest and then use these neurons for ... More
Localization of dopamine D1-receptors in vertebrate retinae.
AuthorsBehrens UD, Wagner HJ
JournalNeurochem Int
PubMed ID8574179
We used the fluorescent labelled dopamine D1-receptor antagonist Bodipy-SCH 23390 for the cellular localization of D1-ligand binding sites in the retinae of different vertebrates (teleosts, Xenopus, turtle, rat and rabbit). Competition experiments with unfixed cryosections of fish retina were performed to characterize the binding conditions of Bodipy-labelled SCH 23390. Tissue ... More
Cellular distribution of dopamine D1 and D2 receptors in rat medial prefrontal cortex.
AuthorsVincent SL, Khan Y, Benes FM
JournalJ Neurosci
PubMed ID8501521
The relative distribution and cellular localization of the dopamine D1 and D2 receptor subtypes were assessed in frozen sections of rat medial prefrontal cortex (mPFC). The D1 and D2 receptor binding sites were labeled with the selective high-affinity antagonists SCH 23390 and N-(p-aminophenethyl)-spiperone (NAPS), respectively, coupled to either Bodipy or ... More