Relationship between contact inhibition and intranuclear S100C of normal human fibroblasts.
AuthorsSakaguchi M, Miyazaki M, Inoue Y, Tsuji T, Kouchi H, Tanaka T, Yamada H, Namba M
JournalJ Cell Biol
PubMed ID10851017
'Many lines of evidence indicate that neoplastic transformation of cells occurs by a multistep process. For neoplastic transformation of normal human cells, they must be first immortalized and then be converted into neoplastic cells. It is well known that the immortalization is a critical step for the neoplastic transformation of ... More
CD34+ corneal stromal cells are bone marrow-derived and express hemopoietic stem cell markers.
AuthorsSosnová M, Bradl M, Forrester JV
JournalStem Cells
PubMed ID15790772
'Previous studies have suggested that corneal stromal keratocytes express the CD34 antigen. We wished to investigate CD34 antigen expression in normal mouse cornea using dual- and triple-staining techniques. Whole-mount preparations of mouse and rat corneas were examined with confocal microscopy using single, dual, or triple immunostaining to study their morphology, ... More
Real-time gene expression analysis in human xenografts for evaluation of histone deacetylase inhibitors.
AuthorsBeliën A, De Schepper S, Floren W, Janssens B, Mariën A, King P, Van Dun J, Andries L, Voeten J, Bijnens L, Janicot M, Arts J
JournalMol Cancer Ther
PubMed ID16985066
'Real-time analysis of gene expression in experimental tumor models represents a major tool to document disease biology and evaluate disease treatment. However, monitoring gene regulation in vivo still is an emerging field, and thus far it has not been linked to long-term tumor growth and disease outcome. In this report, ... More
Suppression of Fas/APO-1-mediated apoptosis by mitogen-activated kinase signaling.
AuthorsHolmström TH, Chow SC, Elo I, Coffey ET, Orrenius S, Sistonen L, Eriksson JE
JournalJ Immunol
PubMed ID9510160
'Jurkat T cells undergo rapid apoptosis upon stimulation of the Fas/APO-1 (CD95) receptor. We examined the role of the mitogen-activated protein kinase (MAPK) cascade as a negative regulator of Fas-mediated apoptosis. To this end, we used both physiologic and artificial activators of MAPK, all of which activate MAPK by distinct ... More
Close relation of arterial ICC-like cells to the contractile phenotype of vascular smooth muscle cell.
This work aimed to establish the lineage of cells similar to the interstitial cells of Cajal (ICC), the arterial ICC-like (AIL) cells, which have recently been described in resistance arteries, and to study their location in the artery wall. Segments of guinea-pig mesenteric arteries and single AIL cells freshly isolated ... More
How an actin network might cause fountain streaming and nuclear migration in the syncytial Drosophila embryo.
Authorsvon Dassow G, Schubiger G
JournalJ Cell Biol
PubMed ID7798318
We show here using time-lapse video tapes that cytoplasmic streaming causes nuclear migration along the anterior-posterior axis (axial expansion) in the early syncytial embryo of Drosophila melanogaster. Using confocal microscopy and labeled phalloidin we explore the distribution of F-actin during axial expansion. We find that a network of F-actin fibers ... More
Musculature of the facultative parasite Urastoma cyprinae (Platyhelminthes).
AuthorsHooge MD, Tyler S
JournalJ Morphol
PubMed ID10461131
In an effort to understand how the feeding motions of Urastoma cyprinae are generated, the arrangement of its musculature was studied using fluorescence microscopy of phalloidin-linked fluorescent stains and conventional light histology and transmission electron microscopy. BODIPY 558/568 phalloidin and Alexa 488 phalloidin resolved a meshwork of ribbon-shaped body-wall muscles ... More
Motility and cytoskeletal organization of migrating cerebellar granule neurons.
AuthorsRivas RJ, Hatten ME
JournalJ Neurosci
PubMed ID7869123
To characterize CNS neuronal precursor migration along astroglial fibers, we examined the motility of the migratory leading process and cytoskeletal-based mechanisms of locomotion of early postnatal mouse cerebellar granule neurons in vitro. To visualize the surface motility of the leading process, granule neurons were labeled with the fluorescent lipophilic dye, ... More
Evidence for Rho-mediated agonist stimulation of phospholipase D in rat1 fibroblasts. Effects of Clostridium botulinum C3 exoenzyme.
AuthorsMalcolm KC, Elliott CM, Exton JH
JournalJ Biol Chem
PubMed ID8662844
Small GTP-binding proteins of the Rho family are implicated in the in vitro regulation of phosphatidylcholine hydrolysis by phospholipase D (PLD). However, their role in agonist-stimulated PLD activity in whole cells is not clear. The ribosyltransferase C3 from Clostridium botulinum modifies Rho proteins and inhibits their function. When introduced into ... More
Quantitative image analysis of F-actin in endothelial cells.
AuthorsEhringer WD, Yamany S, Steier K, Farag A, Roisen FJ, Dozier A, Miller FN
JournalMicrocirculation
PubMed ID10654280
OBJECTIVE: Filamentous actin (F-actin) plays a central role in maintaining endothelial barrier function. Thrombin and histamine, two inflammatory mediators that increase endothelial permeability, can alter F-actin production and distribution. In this study, we use a newly developed image analysis technique to show that these two inflammatory mediators differentially alter F-actin ... More
Analysis of platelet-derived growth factor-induced phospholipase D activation in mouse embryo fibroblasts lacking phospholipase C-gamma1.
AuthorsHess JA, Ji QS, Carpenter G, Exton JH
JournalJ Biol Chem
PubMed ID9685408
Platelet-derived growth factor (PDGF) activates phospholipase D (PLD) in mouse embryo fibroblasts (MEFs). In order to investigate a role for phospholipase C-gamma1 (PLC-gamma1), we used targeted disruption of the Plcg1 gene in the mouse to develop Plcg1(+/+) and Plcg1(-/-) cell lines. Plcg1(+/+) MEFs treated with PDGF showed a time- and ... More
The CHORD protein CHP-1 regulates EGF receptor trafficking and signaling in C. elegans and in human cells.
Authors
JournalElife
PubMed ID32053105
Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells.
Authors
JournalInt J Mol Sci
PubMed ID31947656
Secretome of endothelial progenitor cells from stroke patients promotes endothelial barrier tightness and protects against hypoxia-induced vascular leakage.
Authors
JournalStem Cell Res Ther
PubMed ID34702368
Quantitative analysis and virulence phenotypes of atypical enteropathogenic Escherichia coli (EPEC) acquired from diarrheal stool samples from a Midwest US hospital.
Authors
JournalGut Microbes
PubMed ID33131419
pH-Sensing G Protein-Coupled Receptor OGR1 (GPR68) Expression and Activation Increases in Intestinal Inflammation and Fibrosis.
Authors
JournalInt J Mol Sci
PubMed ID35163345
Multiple effects of electroporation on the adhesive behaviour of breast cancer cells and fibroblasts.
Authors
JournalCancer Cell Int
PubMed ID22439612
Molecular signaling pathways mediating osteoclastogenesis induced by prostate cancer cells.
Authors
JournalBMC Cancer
PubMed ID24370273
Host CDK-1 and formin mediate microvillar effacement induced by enterohemorrhagic Escherichia coli.
Authors
JournalNat Commun
PubMed ID33397943
Coordination of Receptor Tyrosine Kinase Signaling and Interfacial Tension Dynamics Drives Radial Intercalation and Tube Elongation.
Authors
JournalDev Cell
PubMed ID29634937
Aster-C coordinates with COP I vesicles to regulate lysosomal trafficking and activation of mTORC1.