BacLight™ 细菌膜电位检测试剂盒
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<i>Bac</i>Light&trade; 细菌膜电位检测试剂盒
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BacLight™ 细菌膜电位检测试剂盒

The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and了解更多信息
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货号数量
B349501 个试剂盒
货号 B34950
价格（CNY)
3,570.00
飞享价
Ends: 31-Dec-2025
4,744.00
共减 1,174.00 (25%)
Each
添加至购物车
数量:
1 个试剂盒
价格（CNY)
3,570.00
飞享价
Ends: 31-Dec-2025
4,744.00
共减 1,174.00 (25%)
Each
添加至购物车
The BacLight™ Bacterial Membrane Potential Kit provides the fluorescent membrane-potential indicator dye, DiOC2(3), along with a proton ionophore (CCCP) and premixed buffer. DiOC2(3) at low concentrations exhibits green fluorescence in all bacterial cells, but it becomes more concentrated in healthy cells that are maintaining a membrane potential, causing the dye to self-associate and the fluorescence emission to shift to red. The red- and green-fluorescent bacterial populations are easily distinguished using a flow cytometer. CCCP is included in the kit for use as a control because it eradicates the proton gradient, eliminating bacterial membrane potential.

View additional information about all microbiology assays for flow cytometry.
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型膜电位探针
产品规格管
数量1 个试剂盒
运输条件湿冰
发射482⁄497
适用于（应用）膜电位测定试剂盒
适用于（设备）流式细胞仪
产品线BacLight™
产品类型细菌膜电位测定试剂盒
Unit SizeEach
内容与储存
含有 DiOC2(3)（1.2 mL 3 mM 的 DMSO 溶液）、CCCP（300 μL 500 μM 的 DMSO 溶液）和 PBS 悬液 (200 mL)。 避光储存在冷藏冰箱 (2–8°C) 中。

常见问题解答 (FAQ)

我可以在流式细胞仪上看到哪些细菌参数?

您可以用BacLight Green细菌染色剂（货号B35000）或BacLight Red细菌染色剂（货号B35001）等常规染色剂进行细菌染色。你们可以看到革兰氏特性（货号L7005），细胞存活率（货号L7007、L7012和L13152），细胞计数（货号L34856和B7277）和细胞活力。细胞活力可以通过膜电位法（货号B34950）或代谢法（货号B34954和B34956）测定。

我可通过流式细胞仪观察细菌和酵母么？

可以。流式细胞仪可用于：

•计数检测：用于流式细胞仪的细菌计数试剂盒（货号B7277）或LIVE/DEAD BacLight细菌活性和计数试剂盒（货号L34856）
•活性/活力检测：LIVE/DEAD BacLight 细菌活性试剂盒（货号 L13152）
•膜电位：BacLight细菌膜电位试剂盒（货号B34950）
•酵母活性/活力实验：用于流式细胞仪（货号L34952）的LIVE/DEAD FungaLight酵母活性试剂盒，FungaLight酵母CFDA, AM/碘化丙啶活力试剂盒（货号F34953）。

What bacterial parameters can I look at by flow cytometry?

You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I look at bacteria and yeast on a flow cytometer?

Yes, you can. We offer:

-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.