Invitrogen

BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor™ 488

Name: BrdU Monoclonal Antibody (MoBU-1), Alexa Fluor\u2122 488
Brand: Invitrogen
SKU: B35130

Advanced Verification

22 Published Figures

49 References

View all (60) BrdU antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (22)
Advanced Verification (1)

BrdU Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 23

BrdU Antibody (B35130) in ICC/IF

Aging cell 2021 - FIGURE 1 Deep senescence (DS) in primary adult human mammary fibroblasts is reversible. (a) Schematic illustrating epithelial and fibroblast senescence and the DS reversal strategy. (b) Early proliferating (EP) fibroblasts at P11 were serially passaged until they reached senescence at P29. Deeply senescent (DS) fibroblasts were defined as a population which did not expand when kept in culture for 3 weeks po... View More

BrdU Antibody in Immunohistochemistry (Frozen) (IHC (F))

BrdU Antibody in Immunohistochemistry (IHC)

Product Image Gallery

BrdU Antibody — Figure 1 FGF 2 impairs cell cycle progression in K-Ras-driven cancer cells. Serum-starved Y1D1 cells were stimulated by 10% serum with or without 10 ng*mL -1 FGF 2 to re-entry the cell cycle. Cells were subjected to a BrdU pulse 30 min before sample collection (every two hours). (A) Representative zebra plot flow cytometry data of BrdU-stained cells versus DNA content at the indicated times after stimulation comparing cell cycle re-entry and progression with or without FGF 2. BrdU was added at 50 mu m for 30 min before harvesting. The arrows indicate BrdU-unlabeled S-phase cells. (B) Time-course flow cytometry analyses comparing the progression along cell cycle phases from 2 to 48 h after stimulation by serum with or without FGF 2. BrdU was added at 50 mu m for 30 min before harvesting. (C) Quantifications of flow cytometry data showing phospho-histone H3 (S10) and DNA content double stain. The proportions of cells in each phase were measured 24, 28, 32, and 36 h after stimulation with serum or serum + FGF 2 in presence or absence of 2 mu m colchicine. Mitotic cells were addressed by chromatin condensation indicated by phospho-histone H3 (S10)-positive stain. Asterisks indicate significant differences. *** P <= 0.001 (one-way ANOVA of variance followed by multiple comparison post-test) ( n = 4). {TM}

Product Details

B35130

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-dependent

View 9 publications 9 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 19 publications 19 publications

Flow Cytometry (Flow)

View 16 publications 16 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Human, Mouse

Host/Isotype

Mouse / IgG

Class

Monoclonal

Type

Antibody

Clone

MoBU-1

Immunogen

BrdU

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

5mM sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536434

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more