Dicer inactivation leads to progressive functional and structural degeneration of the mouse retina.
AuthorsDamiani D, Alexander JJ, O'Rourke JR, McManus M, Jadhav AP, Cepko CL, Hauswirth WW, Harfe BD, Strettoi E,
JournalJ Neurosci
PubMed ID18463241
'MicroRNAs (miRNAs) are small, highly conserved molecules that have been shown to regulate the expression of genes by binding to specific target mRNAs. Dicer, an RNase III endonuclease, is essential for the production and function of mature miRNAs, and removal of Dicer has been shown to disrupt many developmental processes. ... More
The distribution of polycomb-group proteins during cell division and development in Drosophila embryos: impact on models for silencing.
AuthorsBuchenau P, Hodgson J, Strutt H, Arndt-Jovin DJ
JournalJ Cell Biol
PubMed ID9548724
'The subcellular three-dimensional distribution of three polycomb-group (PcG) proteins-polycomb, polyhomeotic and posterior sex combs-in fixed whole-mount Drosophila embryos was analyzed by multicolor confocal fluorescence microscopy. All three proteins are localized in complex patterns of 100 or more loci throughout most of the interphase nuclear volume. The rather narrow distribution of ... More
Loss of binding and entry of liposome-DNA complexes decreases transfection efficiency in differentiated airway epithelial cells.
Authors Matsui H; Johnson L G; Randell S H; Boucher R C;
JournalJ Biol Chem
PubMed ID8995411
The target cells for gene therapy of cystic fibrosis lung disease are the well differentiated cells that line airway lumens. Employing cultures of airway epithelial cells that grow like ... More
Characterization of DNA/lipid complexes by fluorescence resonance energy transfer.
AuthorsMadeira C, Loura LM, Aires-Barros MR, Fedorov A, Prieto M
JournalBiophys J
PubMed ID14581211
Fluorescence resonance energy transfer (FRET) is a potential method for the characterization of DNA-cationic lipid complexes (lipoplexes). In this work, we used FRET models assuming a multilamellar lipoplex arrangement. The application of these models allows the determination of the distance between the fluorescent intercalator on the DNA and a membrane ... More
Laser-emission imaging of nuclear biomarkers for high-contrast cancer screening and immunodiagnosis.
Authors
JournalNat Biomed Eng
PubMed ID29204310
CUBIC-Cloud provides an integrative computational framework toward community-driven whole-mouse-brain mapping.
Authors
JournalCell Rep Methods
PubMed ID35475238
Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues.