bottleneck acts as a regulator of the microfilament network governing cellularization of the Drosophila embryo.
AuthorsSchejter ED, Wieschaus E
JournalCell
PubMed ID8402919
'A dynamic network of cortical microfilaments is associated with the cleavage furrow membranes during cellularization of the Drosophila embryo. A specific set of structural rearrangements in this network is required for orchestration and execution of its mechanistic roles. We describe the characterization of the gene bottleneck (bnk), mutations in which ... More
Confocal laser scanning microscopy of rat follicle development.
AuthorsZucker RM, Keshaviah AP, Price OT, Goldman JM
JournalJ Histochem Cytochem
PubMed ID10820152
This study used confocal laser scanning microscopy (CLSM) to study follicular development in millimeter pieces of rat ovary. To use this technology, it is essential to stain the tissue before laser excitation with the confocal microscope. Various fluorescent stains (Yo-Pro, Bo-Pro, LysoTracker Red, hydroethidine, ethidium bromide, and 7-aminoactinomycin-d) were applied ... More
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections.
AuthorsBink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Höfler H, Werner M
JournalHistochem Cell Biol
PubMed ID11405057
Confocal laser scanning microscopy (CLSM) is an extensive but reliable tool for assessing the hybridisation signals in fluorescence in situ hybridisation (FISH). Most CLSMs are equipped with an argon-laser and a helium/neon-laser illumination system with excitation wavelengths of 488, 543 and 633 nm. A protocol for an optimal nuclear counterstaining ... More