A quantum dot-aptamer beacon using a DNA intercalating dye as the FRET reporter: application to label-free thrombin detection.
AuthorsChi CW, Lao YH, Li YS, Chen LC,
JournalBiosens Bioelectron
PubMed ID21306887
'A new quantum dot (QD)-aptamer (apt) beacon that acts by folding-induced dissociation of a DNA intercalating dye, BOBO-3(B), is demonstrated with label-free thrombin detection. The beacon, denoted as QD-apt:B, is constructed by (1) coupling of a single-stranded thrombin aptamer to Qdot 565 via EDC/Sulfo-NHS chemistry and (2) staining the duplex ... More
A method for staining of cell nuclei in Xenopus laevis embryos with cyanine dyes for whole-mount confocal laser scanning microscopy.
Authorsde Mazière AM, Hage WJ, Ubbels GA
JournalJ Histochem Cytochem
PubMed ID8601700
'To study the cell cleavage pattern in experimentally treated Xenopus laevis blastulae, we devised a method to visualize all cell nuclei, whether in interphase or in a mitotic phase, in whole-mount embryos using confocal laser scanning microscopy. Optimal staining conditions were defined for the recently commercialized cyanine nucleic acid stain ... More
Binding of BOBO-3 intercalative dye to DNA homo-oligonucleotides with different base compositions.
AuthorsRuedas-Rama MJ, Alvarez-Pez JM, Paredes JM, Talavera EM, Orte A,
JournalJ Phys Chem B
PubMed ID20415455
'The interactions between trimethine cyanine homodimer dye, BOBO-3 (1,1''-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-methyl-2,3-dihydro-(benzo-1,3-thiazole)-2-methylidene]-pyridinium tetraiodide), and single-stranded homo-oligonucleotides, double-stranded complementary homo-oligonucleotides, and high-molecular-weight double-stranded polyhomonucleotides have been investigated in detail using absorption and both steady-state and time-resolved fluorescence spectroscopy. In this work, we describe the differences in the binding behavior of BOBO-3 with double-stranded DNA ... More
Formation of stable BOBO-3 H-aggregate complexes hinders DNA hybridization.
AuthorsRuedas-Rama MJ, Alvarez-Pez JM, Orte A,
JournalJ Phys Chem B
PubMed ID20572644
'In recent works, we have been studying the photophysics and binding properties of the trimethine cyanine homodimer dye BOBO-3, a DNA intercalative fluorophore that shows an important fluorescence enhancement upon binding to double-stranded DNA. During the course of studying the interactions of the dye with single-stranded homo-oligonucleotides we detected the ... More
Human age-related cataract and lens epithelial cell death.
AuthorsHarocopos GJ, Alvares KM, Kolker AE, Beebe DC
JournalInvest Ophthalmol Vis Sci
PubMed ID9856780
PURPOSE: To evaluate the importance of lens epithelial cell death in age-related cataract. To determine whether the large percentage of terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL)-positive lens epithelial cells previously reported in human capsulotomy specimens results from apoptosis or necrosis. METHODS: Capsulotomy specimens from patients who had undergone ... More
Stains, labels and detection strategies for nucleic acids assays.
AuthorsKricka LJ
JournalAnn Clin Biochem
PubMed ID11928759
Selected developments and trends in stains, labels and strategies for detecting and measuring nucleic acids (DNA, RNA) and related molecules [e.g. oligo(deoxy)nucleotides, nucleic acid fragments and polymerase chain reaction products] are surveyed based on the literature in the final decade of the 20th century (1991-2000). During this period, important families ... More
The TRAPP complex mediates secretion arrest induced by stress granule assembly.
Authors
JournalEMBO J
PubMed ID31429971
Regulation of cortical activity and arousal by the matrix cells of the ventromedial thalamic nucleus.
Authors
JournalNat Commun
PubMed ID29844415
Phenol-Soluble Modulins From Staphylococcus aureus Biofilms Form Complexes With DNA to Drive Autoimmunity.
Authors
JournalFront Cell Infect Microbiol
PubMed ID35646719
Isolation of Rab5-positive endosomes reveals a new mitochondrial degradation pathway utilized by BNIP3 and Parkin.
Authors
JournalSmall GTPases
PubMed ID28696827
Higher Arc Nucleus-to-Cytoplasm Ratio during Sleep in the Superficial Layers of the Mouse Cortex.