1个试剂盒足以用于150个显微镜玻片 (18 x 18 mm),其中含有:封闭缓冲液 (1X),22.5 mL
聚-HRP 偶联山羊抗兔二抗 (1X),22.5 mL
生物素-XX 酪胺试剂
过氧化氢
常见问题解答 (FAQ)
With a SuperBoost tyramide kit, I got excessive and non-specific labeling. What can I do to limit background and acquire a more localized labeling?
To limit background, we recommend performing a pre-blocking step with 3% H2O2 for 60 mins to inactivate endogenous peroxidases. To limit the localization of labeling, we recommend optimizing the final concentration of the primary and secondary antibodies and the dye-tyramide. You may also limit the incubation time of the dye-tyramide on the sample.
How are SuperBoost tyramide kits different from the original TSA labeling kits?
The SuperBoost tyramide kits utilize poly-HRP labeled antibodies. This provides a greater number of horseradish peroxidase (HRP) molecules per antibody. The original kits used antibodies and streptavidin that were directly conjugated with HRP and thus, limited the number per antibody or streptavidin.