•封闭缓冲液 (1 X),22.5 mL • HRP 结合型链霉素亲和素 (1 X),22.5 mL • Alexa Fluor 酪胺试剂 • 过氧化氢(稳定 3% 溶液),28.5 mL • 反应缓冲液 (20 X),6 mL • 反应终止液,8 mg • 二甲亚砜 (DMSO),200 µL
在 2°-8°C 下储存。根据说明储存时,产品在接收后可保持稳定 6 个月。
常见问题解答 (FAQ)
With a SuperBoost tyramide kit, I got excessive and non-specific labeling. What can I do to limit background and acquire a more localized labeling?
To limit background, we recommend performing a pre-blocking step with 3% H2O2 for 60 mins to inactivate endogenous peroxidases. To limit the localization of labeling, we recommend optimizing the final concentration of the primary and secondary antibodies and the dye-tyramide. You may also limit the incubation time of the dye-tyramide on the sample.
How are SuperBoost tyramide kits different from the original TSA labeling kits?
The SuperBoost tyramide kits utilize poly-HRP labeled antibodies. This provides a greater number of horseradish peroxidase (HRP) molecules per antibody. The original kits used antibodies and streptavidin that were directly conjugated with HRP and thus, limited the number per antibody or streptavidin.