Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence.
AuthorsWang H, Pangilinan RL, Zhu Y
JournalMethods Mol Biol
PubMed ID31939172
Tyramide signal amplification (TSA) is an enzyme-mediated method to enhance the immunohistochemical detection of protein, nucleic acid, or other molecules in situ.Here we describe immunofluorescent detection of a low-abundance cytokine receptor, interleukin-17 receptor B (IL17RB) in U2OS cells, using tyramide signal amplification. In addition, we present a tyramide signal amplification ... More