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查看更多产品信息 Bolt® Mini Gel Tank - FAQs (B4477599)
23 个常见问题解答
小型转印模块专为小型凝胶电泳槽设计。该模块也适用于Bolt小型凝胶电泳槽（2014年12月31日起停产），但不适用于XCell SureLock Mini Cell或其他供应商生产的电泳槽。
Bolt小型凝胶电泳槽可最多同时运行2个转印模块，每次运行最多转印2块凝胶。
Bolt小型凝胶电泳槽已于2014年12月31日停产，库存售尽后将停止供应。为升级Bolt小型凝胶电泳槽以用于新型Bolt Bis-Tris Plus凝胶的电泳，应将黑色10.5 cm凝胶盒夹凸轮手柄更换为灰色10 cm凝胶盒夹凸轮手柄（货号A26732）。凸轮手柄更换说明见视频（https://www.youtube.com/watch?v=1FtiX8Skllw）。点击此处（https://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/protein-gel-electrophoresis/electrophoresis-chambers/mini-gel-tank/bolt-mini-gel-tank-resources.html），可获取更多资源。
若10 cm凝胶盒的小型凝胶使用Bolt小型凝胶电泳槽进行电泳（不将10.5 cm凝胶盒夹凸轮手柄更换为10 cm凝胶盒夹凸轮手柄），请参考使用手册（https://tools.thermofisher.com/content/sfs/manuals/mini_gel_tank_man.pdf）第22页中的说明。
为获得最佳结果，10 cm凝胶盒的小型凝胶使用Bolt小型凝胶电泳槽电泳时，应将Bolt小型凝胶电泳槽上的黑色10.5 cm凝胶盒夹凸轮手柄更换为灰色10 cm凝胶盒夹凸轮手柄（货号A26732）。凸轮手柄更换说明见使用手册第20页或视频（https://www.youtube.com/watch?v=1FtiX8Skllw）。点击此处（https://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/protein-gel-electrophoresis/electrophoresis-chambers/mini-gel-tank/bolt-mini-gel-tank-resources.html），可获取更多资源。
注意：Bolt小型凝胶电泳槽已于2014年12月31日停产，库存售尽后将停止供应。
我们的PowerEase 90W电源可兼容Bolt小型凝胶电泳槽。PowerEase 500电源（2013年12月31日起停产）也能兼容Bolt小型凝胶电泳槽。Invitrogen电源适配器随电泳槽一起提供，用于不能与隐蔽式或不可伸缩式电源线连接使用的电源。
注意：Bolt小型凝胶电泳槽已于2014年12月31日停产，库存售尽后将停止供应。
我们建议：
•电泳完毕后，妥善处理电泳缓冲液。用水冲洗电泳槽以除去残留的缓冲液。
•用蘸过水的柔软非摩擦性无尘布清洁Bolt 小型凝胶电泳槽的表面。
•不要使用强力洗涤剂或溶剂清洗装置。
注意：Bolt 小型凝胶电泳槽已于2014年12月31日停产，库存售尽后将停止供应。
Bolt小型凝胶电泳槽不受酒精的影响，但不能兼容氯化烃（如氯仿）、芳香烃（如甲苯和苯）或丙酮。
注意：Bolt小型凝胶电泳槽已于2014年12月31日停产，库存售尽后将停止供应。
我们的传统Bolt Bis-Tris Plus小型凝胶（货号BGxxxxxBOX，2014年12月31日起停产）只能使用Bolt小型凝胶电泳槽（2014年12月31日起停产，库存售尽后将停止供应）进行电泳。
若Bolt Bis-Tris Plus小型凝胶（货号NWxxxxxBOX）以及Invitrogen小型凝胶和NuPAGE小型凝胶使用Bolt小型凝胶电泳槽进行电泳，则有必要升级电泳槽，可将黑色10.5 cm凝胶盒夹凸轮手柄更换为灰色10 cm凝胶盒夹凸轮手柄（货号A26732，凝胶盒夹凸轮手柄装置）。凸轮手柄更换说明见视频（https://www.youtube.com/watch?v=1FtiX8Skllw)。点击此处(https://www.thermofisher.com/us/en/home/life-science/protein-expression-and-analysis/protein-gel-electrophoresis/electrophoresis-chambers/mini-gel-tank/bolt-mini-gel-tank-resources.html)，可获取更多资源。
若10 cm凝胶盒的小型凝胶使用Bolt小型凝胶电泳槽进行电泳（不将10.5 cm凝胶盒夹凸轮手柄更换为10 cm凝胶盒夹凸轮手柄），请参考使用手册（https://tools.thermofisher.com/content/sfs/manuals/mini_gel_tank_man.pdf）第22页中的说明。
DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.
Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.
Yes. While we would prefer that you use our devices, Bolt gels can also be transferred using devices from Bio-Rad, including the Mini Trans-Blot Cell, Trans-Blot SD Semi-Dry Transfer Cell, or Trans-Blot Turbo Transfer System.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Yes. You can use the iBlot Dry Transfer System or XCell II Blot Module with SureLock tank and achieve similar transfer efficiency.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Mini Blot Module is designed exclusively for the Mini Gel Tank. It will also fit in the Bolt Mini Gel Tank (discontinued as of December 31, 2014) but will not fit in the XCell SureLock Mini Cell or other vendors' electrophoresis tanks.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Up to two blot modules can be run simultaneously in the Bolt Mini Gel Tank to transfer up to two gels per run.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Bolt Mini Gel Tank has been discontinued as of December 31, 2014, and will be offered until inventory is depleted. To upgrade the Bolt Mini Gel Tank to run the new Bolt Bis-Tris Plus gels as well as other Invitrogen Mini gels, you have to replace the black 10.5 cm cassette clamp cam handles with gray 10 cm cassette clamp cam handles (Cat. No. A26732). Instructions for replacement of the cam handles can be found in this video.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
To run Mini gels with 10 cm gel cassettes using a Bolt Mini Gel Tank (without replacement of 10.5 cm cassette clamp cam handles with 10 cm cassette clamp cam handles), please use the instructions provided on Page 22 of the manual.
For optimal results, to run 10 cm cassette Mini gels with a Bolt Mini Gel Tank, you should replace the black 10.5 cm cassette clamp cam handles on the Bolt Mini Gel Tank with gray 10 cm cassette clamp cam handles (Cat. No. A26732). Instructions for replacement of the cam handles can be found on Page 20 of the manual or in this video. Additional resources can be found here. (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-gel-electrophoresis/protein-gel-electrophoresis-chamber-systems/mini-gel-tank/resources-upgrading-bolt-mini-gel-tank.html)
Note: The Bolt Mini Gel Tank has been discontinued as of December 31, 2014, and will be offered until inventory is depleted.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We offer the PowerEase 90W Power supply that is compatible with the Bolt Mini Gel Tank. The PowerEase 500 Power Supply (discontinued as of December 31, 2013) is also compatible with the Bolt Mini Gel Tank). Invitrogen Power Supply Adapters are supplied with the tank for power supplies that are not designed for use with covered or non-retractable power leads.
Note: The Bolt Mini Gel Tank has been discontinued as of December 31, 2014, and will be offered until inventory is depleted.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
We recommend the following:
When electrophoresis is complete, dispose of the buffer appropriately. Rinse the tank to remove residual buffer.
Clean the surface of the Bolt Mini Gel Tank with a soft non-abrasive, lint-free cloth dampened with water.
Do not use harsh detergents or organic solvents to clean the unit.
Note: The Bolt Mini Gel Tank has been discontinued as of December 31, 2014, and will be offered until inventory is depleted.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
The Bolt Mini Gel Tank is impervious to alcohol, but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene) or acetone.
Note: The Bolt Mini Gel Tank has been discontinued as of December 31, 2014, and will be offered until inventory is depleted.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Our Original Bolt Bis-Tris Plus Mini gels (Cat. No. BGxxxxxBOX, discontinued as of December 31, 2014) can only be run in the Bolt Mini Gel Tank (discontinued as of December 31, 2014, and will be offered until inventory is depleted).
To run our new Bolt Bis-Tris Plus Mini gels (Cat. No. NWxxxxxBOX), as well as our Invitrogen Mini gels and NuPAGE Mini gels using the Bolt Mini Gel Tank, upgrading of the tank is necessary by replacing the black 10.5 cm cassette clamp cam handles with gray 10 cm cassette clamp cam handles (Cat. No. A26732, Cassette Clamp Cam Handle Set). Instructions for replacement of the cam handles can be found in this video (https://www.youtube.com/watch?v=1FtiX8Skllw).
To run Mini gels with 10 cm gel cassettes using the Bolt Mini Gel Tank (without replacement of 10.5 cm cassette clamp cam handles with 10 cm cassette clamp cam handles), please use the instructions provided on Page 22 of the manual (https://tools.thermofisher.com/content/sfs/manuals/mini_gel_tank_man.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.