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Bolt™ Tank Base - FAQs

查看更多产品信息 Bolt™ Tank Base - FAQs (B4478640)

10 个常见问题解答

我能否购买小型转印模块的替换部件？

以下是我们可提供的小型转印模块替换部件：

•切胶刀，货号EI9010
•Bolt电泳槽基座，货号B4478640
•凝胶电泳槽，货号B4478641
•Bolt电泳槽盖，货号B4478591
•凝胶盒夹，右，货号B4478592
•凝胶盒夹，左，货号B4478593
•电源适配器，货号ZA10001

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Which power supplies are compatible with the Mini Gel Tank?

The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are Bolt gels compatible with transfer devices from other suppliers?

Yes. While we would prefer that you use our devices, Bolt gels can also be transferred using devices from Bio-Rad, including the Mini Trans-Blot Cell, Trans-Blot SD Semi-Dry Transfer Cell, or Trans-Blot Turbo Transfer System.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are Bolt gels compatible with other transfer devices?

Yes. You can use the iBlot Dry Transfer System or XCell II Blot Module with SureLock tank and achieve similar transfer efficiency.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I purchase replacement parts for the Mini Blot Module?

Here are the replacement parts we offer for the Mini Blot Module:

- Mini Blot Module Gasket, Cat. No. B1001
- Gel Knife, Cat. No. EI9010
- Bolt Tank Base, Cat. No. B4478640
- Gel Runner Tank, Cat. No. B4478641
- Mini Gel Tank Lid, Cat. No. A25944
- Cassette Clamp, Right, Cat. No. B4478592
- Cassette Clamp, Left, Cat. No. B4478593
- Power Supply Adapters, Cat. No. ZA10001

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.