JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID10903717
Chlorotoxin inhibits glioma cell invasion via matrix metalloproteinase-2.
AuthorsDeshane J, Garner CC, Sontheimer H
JournalJ Biol Chem
PubMed ID12454020
'Primary brain tumors (gliomas) have the unusual ability to diffusely infiltrate the normal brain thereby evading surgical treatment. Chlorotoxin is a scorpion toxin that specifically binds to the surface of glioma cells and impairs their ability to invade. Using a recombinant His-Cltx we isolated and identified the principal Cltx receptor ... More
Presenilin-1 affects trafficking and processing of betaAPP and is targeted in a complex with nicastrin to the plasma membrane.
AuthorsKaether C, Lammich S, Edbauer D, Ertl M, Rietdorf J, Capell A, Steiner H, Haass C
JournalJ Cell Biol
PubMed ID12147673
'Amyloid beta-peptide (Abeta) is generated by the consecutive cleavages of beta- and gamma-secretase. The intramembraneous gamma-secretase cleavage critically depends on the activity of presenilins (PS1 and PS2). Although there is evidence that PSs are aspartyl proteases with gamma-secretase activity, it remains controversial whether their subcellular localization overlaps with the cellular ... More
Cytoplasmic dynein regulation by subunit heterogeneity and its role in apical transport.
AuthorsTai AW, Chuang JZ, Sung CH
JournalJ Cell Biol
PubMed ID11425878
'Despite the existence of multiple subunit isoforms for the microtubule motor cytoplasmic dynein, it has not yet been directly shown that dynein complexes with different compositions exhibit different properties. The 14-kD dynein light chain Tctex-1, but not its homologue RP3, binds directly to rhodopsin''s cytoplasmic COOH-terminal tail, which encodes an ... More
Aberrant trafficking of the B cell receptor Ig-alpha beta subunit in a B lymphoma cell line.
AuthorsCondon C, Hourihane SL, Dang-Lawson M, Escribano J, Matsuuchi L
JournalJ Immunol
PubMed ID10903747
'The B cell Ag receptor (BCR) has two important functions: first, it binds and takes up Ag for presentation to T lymphocytes; and second, it transmits signals that regulate B cell development. Normal expression of the BCR requires the association of the Ag binding subunit, membrane IgM (mIgM), with the ... More
Interaction with telencephalin and the amyloid precursor protein predicts a ring structure for presenilins.
AuthorsAnnaert WG, Esselens C, Baert V, Boeve C, Snellings G, Cupers P, Craessaerts K, De Strooper B
JournalNeuron
PubMed ID11719200
'The carboxyl terminus of presenilin 1 and 2 (PS1 and PS2) binds to the neuron-specific cell adhesion molecule telencephalin (TLN) in the brain. PS1 deficiency results in the abnormal accumulation of TLN in a yet unidentified intracellular compartment. The first transmembrane domain and carboxyl terminus of PS1 form a binding ... More
Rapid transport of internalized P-selectin to late endosomes and the TGN: roles in regulating cell surface expression and recycling to secretory granules.
AuthorsStraley KS, Green SA
JournalJ Cell Biol
PubMed ID11018057
'Prior studies on receptor recycling through late endosomes and the TGN have suggested that such traffic may be largely limited to specialized proteins that reside in these organelles. We present evidence that efficient recycling along this pathway is functionally important for nonresident proteins. P-selectin, a transmembrane cell adhesion protein involved ... More
Rab 7: an important regulator of late endocytic membrane traffic.
AuthorsFeng Y, Press B, Wandinger-Ness A
JournalJ Cell Biol
PubMed ID8522602
'Rab5 and rab7 proteins belong to a superfamily of small molecular weight GTPases known to be associated with early and late endosomes, respectively. The rab5 protein plays an important regulatory role in early endocytosis, yet the function of rab7 protein was previously uncharacterized. This question was addressed by comparing the ... More
Proteolytic activation of respiratory syncytial virus fusion protein. Cleavage at two furin consensus sequences.
AuthorsZimmer G, Budz L, Herrler G
JournalJ Biol Chem
PubMed ID11418598
'The F (fusion) protein of the respiratory syncytial viruses is synthesized as an inactive precursor F(0) that is proteolytically processed at the multibasic sequence KKRKRR(136) into the subunits F(1) and F(2) by the cellular protease furin. This maturation process is essential for the F protein to gain fusion competence. We ... More
Inhibition of platelet endothelial cell adhesion molecule-1 synthesis and leukocyte transmigration in endothelial cells by the combined action of TNF-alpha and IFN-gamma.
AuthorsRival Y, Del Maschio A, Rabiet MJ, Dejana E, Duperray A
JournalJ Immunol
PubMed ID8757631
'Endothelial cell (EC) junctions regulate circulating leukocyte extravasation and infiltration at inflammatory sites. Several lines of evidence show that platelet endothelial cell adhesion molecule-1 (PECAM-1), a specific component of EC junctions, is required for leukocyte transmigration through EC monolayers. In this paper, we examined the effects of two inflammatory cytokines, ... More
Comparison of methods for erythroblast selection: application to selecting fetal erythroblasts from maternal blood.
AuthorsCollarini EJ, Cain CA, Gammon D, Harriman B, Magee K, Du G, Schueler PA, Mahoney WC
JournalCytometry
PubMed ID11746096
'BACKGROUND: Many methods have been employed to obtain fetal cells from maternal blood for prenatal diagnostics, but there has been little work done that compares the efficacy of different methods. This study presents a comparison of two commonly used methods for selecting erythroblasts with selection directly from whole blood. METHODS: ... More
Post-translational fate of a mucin-like leukocyte sialoglycoprotein (CD43) aberrantly expressed in a colon carcinoma cell line.
AuthorsBaeckström D
JournalJ Biol Chem
PubMed ID9111064
'This paper describes the biosynthesis of L-CanAg, a mucin-like glycoprotein which carries the carcinoma-associated carbohydrate epitope sialyl-Lewis a and is secreted by the colon adenocarcinoma cell line COLO 205. Recently, it has been shown that L-CanAg is a novel glycoform of CD43, a surface sialoglycoprotein normally found only on hematopoietic ... More
Selective reentry of recycling cell surface glycoproteins to the biosynthetic pathway in human hepatocarcinoma HepG2 cells.
AuthorsVolz B, Orberger G, Porwoll S, Hauri HP, Tauber R
JournalJ Cell Biol
PubMed ID7622556
'Return of cell surface glycoproteins to compartments of the secretory pathway has been examined in HepG2 cells comparing return to the trans-Golgi network (TGN), the trans/medial- and cis-Golgi. Transport to these sites was studied by example of the transferrin receptor (TfR) and the serine peptidase dipeptidylpeptidase IV (DPPIV) after labeling ... More
Insulin-responsive aminopeptidase trafficking in 3T3-L1 adipocytes.
AuthorsGarza LA, Birnbaum MJ
JournalJ Biol Chem
PubMed ID10644714
'The insulin-responsive aminopeptidase (IRAP/VP165/gp160) was identified originally in GLUT4-containing vesicles and shown to translocate in response to insulin, much like the glucose transporter 4 (GLUT4). This study characterizes the trafficking and kinetics of IRAP in exocytosis, endocytosis, and recycling to the membrane in 3T3-L1 adipocytes. After exposure of 3T3-L1 adipocytes ... More
The actin cytoskeleton is required for receptor-mediated endocytosis in mammalian cells.
AuthorsLamaze C, Fujimoto LM, Yin HL, Schmid SL
JournalJ Biol Chem
PubMed ID9252336
'Actin filament organization is essential for endocytosis in yeast. In contrast, the actin-depolymerizing agent cytochalasin D has yielded ambiguous results as to a role for actin in receptor-mediated endocytosis in mammalian cells. We have therefore re-examined this issue using highly specific reagents known to sequester actin monomers. Two of these ... More
Biotinylation of reactive amino groups in native recombinant human interleukin-1 beta.
AuthorsYem AW, Zurcher-Neely HA, Richard KA, Staite ND, Heinrikson RL, Deibel MR
JournalJ Biol Chem
PubMed ID2808341
'Recombinant human interleukin-1 beta (rIL-1 beta) was chemically modified by a 10-fold molar excess (reagent:protein) of sulfosuccinimidyl 6-(biotinamido) hexanoate (sulfo-NHS-LC-biotin) or sulfosuccinimidobiotin (sulfo-NHS-biotin) under mild conditions. The primary product was purified in each case by cation exchange high performance liquid chromatography (HPLC) and digested with endoproteinase Lys C. Peptide mapping ... More
Regenerable biosensor platform: a total internal reflection fluorescence cell with electrochemical control.
AuthorsAsanov AN, Wilson WW, Oldham PB
JournalAnal Chem
PubMed ID9530005
'A new biosensor platform that provides simultaneous fluorescence detection and electrochemical control of biospecific binding has been developed and investigated using antibody-antigen and streptavidin-biotin interactions. Specifically, biotin was covalently bound to a transparent indium-tin oxide (ITO) working electrode, which also served as an integral part of a total internal reflection ... More
Effect of integrin beta 2 subunit truncations on LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) assembly, surface expression, and function.
AuthorsTan SM, Hyland RH, Al-Shamkhani A, Douglass WA, Shaw JM, Law SK
JournalJ Immunol
PubMed ID10946284
'LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) are members of the beta2 integrins involved in leukocyte function during immune and inflammatory responses. We aimed to determine a minimized beta2 subunit that forms functional LFA-1 and Mac-1. Using a series of truncated beta2 variants, we showed that the subregion Q23-D300 of the beta2 ... More
Biotin derivatives of D-Phe-Pro-Arg-CH2Cl for active-site-specific labeling of thrombin and other serine proteinases.
AuthorsAnderson PJ, Bock PE
JournalAnal Biochem
PubMed ID11554721
'Biotin derivatives of peptide chloromethyl ketones have ideal properties for specific labeling of the catalytic sites of serine proteinases but have not been widely used as probes because of the difficulty of synthesis and their instability. To make the reagents more accessible, a simple, economical method was developed for preparation ... More
Optimization of cell surface binding enhances efficiency and specificity of molecular conjugate gene delivery.
AuthorsSchaffer DV, Lauffenburger DA
JournalJ Biol Chem
PubMed ID9774415
'Molecular conjugates, or polyplexes, are promising synthetic vectors for targeted, in vivo gene delivery, if their efficiency can be improved. Gaining mechanistic information on conjugate gene delivery can potentially yield significant improvements in transfer efficiency by revealing barriers to conjugate transfer from the cell surface to the nucleus. We have ... More
Purification and characterization of membrane proteins.
AuthorsKraehenbuhl JP, Bonnard C
JournalMethods Enzymol
PubMed ID2167430
CD66 family members are associated with tyrosine kinase activity in human neutrophils.
AuthorsSkubitz KM, Campbell KD, Ahmed K, Skubitz AP
JournalJ Immunol
PubMed ID7594554
'The granulocyte activation Ags, CD66a, CD66b, CD66c, and CD66d, are expressed at low levels on resting blood granulocytes, but their surface expression is up-regulated following stimulation. CD66a, in contrast to CD66b and CD66c which are anchored to the membrane via a glycosyl-phosphatidylinositol linkage, is a transmembrane protein with a cytoplasmic ... More
The polarity of the plasma membrane protein RET-PE2 in retinal pigment epithelium is developmentally regulated.
AuthorsMarmorstein AD, Bonilha VL, Chiflet S, Neill JM, Rodriguez-Boulan E
JournalJ Cell Sci
PubMed ID9004037
'The retinal pigment epithelium (RPE) differs from other epithelia in that the apical surface is not free; instead, it interacts with both photoreceptors and a specialized extracellular material, the interphotoreceptor matrix. Biochemical characterization of the apical and basolateral surfaces of RPE in adult rat eye cups, using a novel in ... More
Characterization of the insulin-regulated membrane aminopeptidase in 3T3-L1 adipocytes.
AuthorsRoss SA, Scott HM, Morris NJ, Leung WY, Mao F, Lienhard GE, Keller SR
JournalJ Biol Chem
PubMed ID8621739
'A novel membrane aminopeptidase has been identified as a major protein in vesicles from rat adipocytes containing the glucose transporter isotype Glut4. In this study we have characterized this aminopeptidase, referred to as vp165, in 3T3-L1 adipocytes. The subcellular distributions of vp165 and Glut4 were determined by immunoisolation of vesicles ... More
A microscale electrospray interface incorporating a monolithic, poly(styrene-divinylbenzene) support for on-line liquid chromatography/tandem mass spectrometry analysis of peptides and proteins.
AuthorsMoore RE, Licklider L, Schumann D, Lee TD
JournalAnal Chem
PubMed ID9852776
'A methodology is described for creating a monolithic chromatography support within a pulled fused-silica electrospray needle. The monolith was formed from a mixture of styrene, divinylbenzene, 1-dodecanol, and toluene using 2,2''-azobis(isobutyronitrile) as the catalyst. The mixture was loaded into 150-micron-i.d. fused-silica capillary tubing with a pulled 5-10-micron needle tip at ... More
Noncooperativity of biotin binding to tetrameric streptavidin.
AuthorsJones ML, Kurzban GP
JournalBiochemistry
PubMed ID7547907
'Streptavidin tetramers have been separated according to their biotin content by anion exchange chromatography. Biotin-free and biotin-saturated streptavidin were coincubated. Streptavidin at intermediate ligation levels, i.e., with one, two, or three molecules of bound biotin, accumulates over time. A steady state distribution of ligation levels is reached after 2 days. ... More
A receptor/cytoskeletal movement triggered by costimulation during T cell activation.
AuthorsWülfing C, Davis MM
JournalScience
PubMed ID9856952
'During T cell activation, the engagement of costimulatory molecules is often crucial to the development of an effective immune response, but the mechanism by which this is achieved is not known. Here, it is shown that beads attached to the surface of a T cell translocate toward the interface shortly ... More
Transferrin-binding protein complex is the receptor for transferrin uptake in Trypanosoma brucei.
AuthorsSteverding D, Stierhof YD, Fuchs H, Tauber R, Overath P
JournalJ Cell Biol
PubMed ID8522581
'In Trypanosoma brucei, the products of two genes, ESAG 6 and ESAG 7, located upstream of the variant surface glycoprotein gene in a polycistronic expression site form a glycosylphosphatidylinositol-anchored transferrin-binding protein (TFBP) complex. It is shown by gel filtration and membrane-binding experiments that the TFBP complex is heterodimeric and binds ... More
Improved nonradioactive cell surface labeling technique for immunoprecipitation.
AuthorsLantz LM, Holmes KL
JournalBiotechniques
PubMed ID7702854
Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin
AuthorsOgawa M, Kosaka N, Choyke PL, Kobayashi H,
JournalBioconjug Chem
PubMed ID19072537
In vivo molecular cancer imaging with monoclonal antibodies has great potential not only for cancer detection, but also for cancer characterization. However, the prolonged retention of intravenously injected antibody in the blood causes low target tumor-to-background ratio (TBR). Avidin has been used as a ... More
Preparation of oligonucleotide-biotin conjugates with cleavable linkers.
AuthorsSoukup GA, Cerny RL, Maher LJ
JournalBioconjug Chem
PubMed ID7711100
A procedure is presented for preparing an oligonucleotide-biotin conjugate that is chemically cleavable through the reduction of a disulfide bond within the linker. Conjugation involves reaction of a primary amine with an N-hydroxysulfosuccinimide ester linked to biotin. The oligonucleotide can be liberated from streptavidin agarose containing immobilized conjugate under mild ... More
AuthorsGuo CY, Shankar RR, Abe S, Ye Z, Thomas RN, Kuo JE
JournalAnal Biochem
PubMed ID1481977
Synthesis of surface-functionalized, probe-containing latex nanospheres is described. Approximately 40,000 probe ions may be encapsulated in a nanosphere of 50 nm diameter. The probe may be a radionuclide or a lanthanide with long-lived fluorescence. Alternatively, a "cargo" of pharmaceutical interest may be used. The surface of each nanosphere contains thousands ... More
Biotinylation: a simple method for labelling complement component C8 with preservation of functional activity.
AuthorsBhakdi S, Roth M, Hugo F
JournalJ Immunol Methods
PubMed ID2754261
Biotinylation of human C8 with the water-soluble biotin derivative biotinylamidohexanoic acid, N-hydroxysulfosuccinimide ester is an excellent method for labelling this terminal complement component with preservation of its functional activity. The biotinylated product can be detected both in native form and also following its incorporation into the terminal complement complexes. Detection ... More
Thy-1 is a component common to multiple populations of synaptic vesicles.
AuthorsJeng CJ, McCarroll SA, Martin TF, Floor E, Adams J, Krantz D, Butz S, Edwards R, Schweitzer ES
JournalJ Cell Biol
PubMed ID9456327
Thy-1, a glycosylphosphatidylinositol-linked integral membrane protein of the immunoglobulin superfamily, is a component of both large dense-core and small clear vesicles in PC12 cells. A majority of this protein, formerly recognized only on the plasma membrane of neurons, is localized to regulated secretory vesicles. Thy-1 is also present in synaptic ... More
Visualization of a specific sequence on a single large DNA molecule using fluorescence microscopy based on a new DNA-stretching method.
AuthorsOana H, Ueda M, Washizu M
JournalBiochem Biophys Res Commun
PubMed ID10548504
A method for analyzing large DNA which makes it possible to obtain spatial information on the positions of specific sequences along a DNA molecule has been developed. Making use of the fact that large DNA molecules are stably elongated under an alternating-current field in a concentrated linear polymer solution, the ... More
Biotinylation reagents for the study of cell surface proteins.
AuthorsElia G,
JournalProteomics
PubMed ID18763706
The extraordinarily stable, non-covalent interaction between avidin and biotin is one of the most commonly exploited tools in chemistry and biology. Methods for derivatization with biotin of a variety of molecules (in particular, proteins) have been introduced, in order to allow their efficient recovery, immobilization and detection with avidin-based reagents. ... More
Synchronized activation and refolding of influenza hemagglutinin in multimeric fusion machines.
AuthorsMarkovic I, Leikina E, Zhukovsky M, Zimmerberg J, Chernomordik LV
JournalJ Cell Biol
PubMed ID11724823
At the time of fusion, membranes are packed with fusogenic proteins. Do adjacent individual proteins interact with each other in the plane of the membrane? Or does each of these proteins serve as an independent fusion machine? Here we report that the low pH-triggered transition between the initial and final ... More
Binding to EGF receptor of a laminin-5 EGF-like fragment liberated during MMP-dependent mammary gland involution.
AuthorsSchenk S, Hintermann E, Bilban M, Koshikawa N, Hojilla C, Khokha R, Quaranta V
JournalJ Cell Biol
PubMed ID12695504
Extracellular matrix (ECM) fragments or cryptic sites unmasked by proteinases have been postulated to affect tissue remodeling and cancer progression. Therefore, the elucidation of their identities and functions is of great interest. Here, we show that matrix metalloproteinases (MMPs) generate a domain (DIII) from the ECM macromolecule laminin-5. Binding of ... More
Phosphatidylserine (PS) induces PS receptor-mediated macropinocytosis and promotes clearance of apoptotic cells.
AuthorsHoffmann PR, deCathelineau AM, Ogden CA, Leverrier Y, Bratton DL, Daleke DL, Ridley AJ, Fadok VA, Henson PM
JournalJ Cell Biol
PubMed ID11706053
Efficient phagocytosis of apoptotic cells is important for normal tissue development, homeostasis, and the resolution of inflammation. Although many receptors have been implicated in the clearance of apoptotic cells, the roles of these receptors in the engulfment process have not been well defined. We developed a novel system to distinguish ... More
Expression of CaT-like, a novel calcium-selective channel, correlates with the malignancy of prostate cancer.
AuthorsWissenbach U, Niemeyer BA, Fixemer T, Schneidewind A, Trost C, Cavalie A, Reus K, Meese E, Bonkhoff H, Flockerzi V
JournalJ Biol Chem
PubMed ID11278579
The regulation of intracellular Ca(2+) plays a key role in the development and growth of cells. Here we report the cloning and functional expression of a highly calcium-selective channel localized on the human chromosome 7. The sequence of the new channel is structurally related to the gene product of the ... More
Identification of a mutation in the ileal sodium-dependent bile acid transporter gene that abolishes transport activity.
AuthorsWong MH, Oelkers P, Dawson PA
JournalJ Biol Chem
PubMed ID7592981
The ileal Na+/bile acid cotransporter plays a critical role in the reabsorption of bile acids from the small intestine. In the course of cloning and characterizing the human ileal Na+/bile acid cotransporter cDNA, a dysfunctional isoform was identified in a patient diagnosed with Crohn's disease. Expression studies using hamster-human ileal ... More
Combining avidin-biotin chemistry with matrix-assisted laser desorption/ionization mass spectrometry.
AuthorsSchriemer DC, Li L
JournalAnal Chem
PubMed ID8843136
Molecular recognition based on the high affinity of avidin for biotin has been combined with matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The rich chemistry of biotinylation reagents underlies the development of a mass tracer method for the rapid and sensitive analysis of biotinylated analyte in complex mixtures. In this method, ... More
Identification of a 40-kDa cell surface sialoglycoprotein with the characteristics of a major influenza C virus receptor in a Madin-Darby canine kidney cell line.
AuthorsZimmer G, Klenk HD, Herrler G
JournalJ Biol Chem
PubMed ID7629082
Infection of cells by influenza C virus is known to be initiated by virus attachment to cell surface glycoconjugates containing N-acetyl-9-O-acetylneuraminic acid. Using an in vitro virus binding assay, we have detected this carbohydrate on several glycoproteins of Madin-Darby canine kidney cells (type I), a polarized epithelial cell line permissive ... More
A fiber-optic evanescent wave DNA biosensor based on novel molecular beacons.
AuthorsLiu X, Tan W
JournalAnal Chem
PubMed ID10575961
We have prepared a novel optical fiber evanescent wave DNA biosensor using a newly developed molecular beacon DNA probe. The molecular beacons (MB) are oligonucleotide probes that become fluorescent upon hybridization with target DNA/RNA molecules. Biotinylated MBs have been designed and immobilized on an optical fiber core surface via biotin-avidin ... More
Regulation of membrane type-1 matrix metalloproteinase activation by proprotein convertases.
AuthorsYana I, Weiss SJ
JournalMol Biol Cell
PubMed ID10888676
Membrane type-1 matrix metalloproteinase (MT1-MMP) is the prototypical member of a subgroup of membrane-anchored proteinases that belong to the matrix metalloproteinase family. Although synthesized as a zymogen, MT1-MMP plays an essential role in extracellular matrix remodeling after an undefined process that unmasks its catalytic domain. We now report the existence ... More
Preclinical evaluation of biotin labeling for red cell survival testing.
AuthorsHoffmann-Fezer G, Trastl C, Beisker W, Berg D, Obermaier J, Kessler W, Mysliwietz J, Schumm M, Filser J, Thierfelder S
JournalAnn Hematol
PubMed ID9200996
Biotin labeling of red cells was tested in dogs as a preclinical study for cell survival. Red cells were labeled with either spacered Biotin-X-NHS (BxNHS) or water-soluble biotin compounds. After reinfusion, biotinylated red cells were detected in small blood samples (5 microliters) with flow cytometry. Improved BxNHS labeling allows an ... More
Immunogold signal amplification: Application of the CARD approach to electron microscopy.
AuthorsMayer G, Bendayan M
JournalJ Histochem Cytochem
PubMed ID10082744
Catalyzed reporter deposition (CARD) is a technique that allows amplification of routine immunolabeling in light microscopy. This procedure takes advantage of the horseradish peroxidase (HRP) from an HRP-avidin complex to catalyze the accumulation of reporter-conjugated tyramine (a phenolic compound) onto a surface displaying biotinylated antigen-antibody complexes. The large amount of ... More
Use of Iodogen and sulfosuccinimidobiotin to identify and isolate cuticular proteins of the filarial parasite Brugia malayi.
AuthorsAlvarez RM, Henry RW, Weil GJ
JournalMol Biochem Parasitol
PubMed ID2725583
The cuticle of filarial nematodes is a dynamic structure which may be an important target for protective host immune responses. Prior studies have employed radioiodination of intact parasites to demonstrate that the collagenous cuticle of filariids contains relatively few exposed proteins, some of which are stage and/or species-specific. In the ... More
Structure of the human macrophage MARCO receptor and characterization of its bacteria-binding region.
AuthorsElomaa O, Sankala M, Pikkarainen T, Bergmann U, Tuuttila A, Raatikainen-Ahokas A, Sariola H, Tryggvason K
JournalJ Biol Chem
PubMed ID9468508
The primary structure of human macrophage receptor with collagenous structure (MARCO) was determined from cDNA clones and shown to be highly similar to that of mouse (Elomaa, O., Kangas, M., Sahlberg, C. , Tuukkanen, J., Sormunen, R., Liakka, A., Thesleff, I., Kraal, G., and Tryggvason, K. (1995) Cell 80, 603-609). ... More
Erbin is a protein concentrated at postsynaptic membranes that interacts with PSD-95.
AuthorsHuang YZ, Wang Q, Xiong WC, Mei L
JournalJ Biol Chem
PubMed ID11279080
Neuregulin is a factor essential for synapse-specific transcription of acetylcholine receptor genes at the neuromuscular junction. Its receptors, ErbB receptor tyrosine kinases, are localized at the postjunctional membrane presumably to ensure localized signaling. However, the molecular mechanisms underlying synaptic localization of ErbBs are unknown. Our recent studies indicate that ErbB4 ... More
Catenin-dependent and -independent functions of vascular endothelial cadherin.
AuthorsNavarro P, Caveda L, Breviario F, Mândoteanu I, Lampugnani MG, Dejana E
JournalJ Biol Chem
PubMed ID8537353
Vascular endothelial cadherin (VE-cadherin, cadherin-5, or 7B4) is an endothelial specific cadherin that regulates cell to cell junction organization in this cell type. Cadherin linkage to intracellular catenins was found to be required for their adhesive properties and for localization at cell to cell junctions. We constructed a mutant form ... More
Histamine treatment induces rearrangements of orthogonal arrays of particles (OAPs) in human AQP4-expressing gastric cells.
AuthorsCarmosino M, Procino G, Nicchia GP, Mannucci R, Verbavatz JM, Gobin R, Svelto M, Valenti G
JournalJ Cell Biol
PubMed ID11564760
To test the involvement of the water channel aquaporin (AQP)-4 in gastric acid physiology, the human gastric cell line (HGT)-1 was stably transfected with rat AQP4. AQP4 was immunolocalized to the basolateral membrane of transfected HGT-1 cells, like in native parietal cells. Expression of AQP4 in transfected cells increased the ... More
A novel model system for characterization of phagosomal maturation, acidification, and intracellular collagen degradation in fibroblasts.
AuthorsArora PD, Manolson MF, Downey GP, Sodek J, McCulloch CA
JournalJ Biol Chem
PubMed ID10945978
Intracellular collagen degradation by fibroblasts is an important but poorly understood pathway for the physiological remodeling of mature connective tissues. The objective of this study was to determine whether gingival fibroblasts that express endogenous alpha(2)beta(1) integrin, the collagen receptor, would exhibit the cellular machinery required for phagosomal maturation and collagen ... More
The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells.
AuthorsYoshimori T, Yamagata F, Yamamoto A, Mizushima N, Kabeya Y, Nara A, Miwako I, Ohashi M, Ohsumi M, Ohsumi Y
JournalMol Biol Cell
PubMed ID10679028
The mouse SKD1 is an AAA-type ATPase homologous to the yeast Vps4p implicated in transport from endosomes to the vacuole. To elucidate a possible role of SKD1 in mammalian endocytosis, we generated a mutant SKD1, harboring a mutation (E235Q) that is equivalent to the dominant negative mutation (E233Q) in Vps4p. ... More
Sulpho-N-hydroxysuccinimide activated long chain biotin. A new microtitre plate assay for the determination of its stability at different pH values and its reaction rate with protein bound amino groups.
AuthorsGrumbach IM, Veh RW
JournalJ Immunol Methods
PubMed ID2066567
Biotinamidohexanoic acid N-hydroxysulphosuccinimide ester (N-hydroxysulphosuccinimide activated long chain biotin; sulpho-NHS-LC-biotin) has become an invaluable tool for the biotinylation of protein despite the absence of data concerning its stability and reaction velocity. A convenient, rapid and sensitive assay for this compound has been developed based on the sulpho-NHS-LC-biotin mediated biotinylation of ... More
Synthesis of the chelator lipid nitrilotriacetic acid ditetradecylamine (NTA-DTDA) and its use with the IAsys biosensor to study receptor-ligand interactions on model membranes.
AuthorsAltin JG, White FA, Easton CJ
JournalBiochim Biophys Acta
PubMed ID11470085
This work describes the synthesis and use of the chelator lipid, nitrilotriacetic acid ditetradecylamine (NTA-DTDA). This lipid is readily dispersed in aqueous media, both alone and when mixed with carrier lipids like dimyristoylphosphatidylcholine (DMPC). Fluorescence microscopic examination of membranes deposited from NTA-DTDA-containing liposomes shows that NTA-DTDA mixes uniformly with the ... More
Lack of oxidative phosphorylation and low mitochondrial membrane potential decrease susceptibility to apoptosis and do not modulate the protective effect of Bcl-x(L) in osteosarcoma cells.
AuthorsDey R, Moraes CT
JournalJ Biol Chem
PubMed ID10702275
We explored the role of low mitochondrial membrane potential (DeltaPsim) and the lack of oxidative phosphorylation in apoptosis by assessing the susceptibility of osteosarcoma cell lines with and without mitochondrial DNA to staurosporine-induced death. Our cells without mitochondrial DNA had low DeltaPsim and no functional oxidative phosphorylation. Contrary to our ... More
Selective accumulation of raft-associated membrane protein LAT in T cell receptor signaling assemblies.
AuthorsHarder T, Kuhn M
JournalJ Cell Biol
PubMed ID11038169
Activation of T cell antigen receptor (TCR) induces tyrosine phosphorylations that mediate the assembly of signaling protein complexes. Moreover, cholesterol-sphingolipid raft membrane domains have been implicated to play a role in TCR signal transduction. Here, we studied the assembly of TCR with signal transduction proteins and raft markers in plasma ... More
Role for the third intracellular loop in cell surface stabilization of the alpha2A-adrenergic receptor.
AuthorsEdwards SW, Limbird LE
JournalJ Biol Chem
PubMed ID10347190
Previous studies have shown that alpha2A-adrenergic receptor (alpha2A-AR) retention at the basolateral surface of polarized MDCKII cells involves its third intracellular (3i loop). The present studies examining mutant alpha2A-ARs possessing short deletions of the 3i loop indicate that no single region can completely account for the accelerated surface turnover of ... More
Glutaredoxin protects cerebellar granule neurons from dopamine-induced apoptosis by activating NF-kappa B via Ref-1.
AuthorsDaily D, Vlamis-Gardikas A, Offen D, Mittelman L, Melamed E, Holmgren A, Barzilai A
JournalJ Biol Chem
PubMed ID11035035
The neurotransmitter dopamine (DA) induces apoptosis via its oxidative metabolites. This study shows that glutaredoxin 2 (Grx2) from Escherichia coli and human glutaredoxin could protect cerebellar granule neurons from DA-induced apoptosis. E. coli Grx2, which catalyzes glutathione-disulfide oxidoreduction via its -Cys-Pro-Tyr-Cys- active site, penetrates into cerebellar granule neurons and exerts ... More
Overexpression of N-acetylglucosaminyltransferase III enhances the epidermal growth factor-induced phosphorylation of ERK in HeLaS3 cells by up-regulation of the internalization rate of the receptors.
AuthorsSato Y, Takahashi M, Shibukawa Y, Jain SK, Hamaoka R, Miyagawa Ji Yaginuma Y, Honke K, Ishikawa M, Taniguchi N
JournalJ Biol Chem
PubMed ID11134020
N-Acetylglucosaminyltransferase III (GnT-III) is a key enzyme that inhibits the extension of N-glycans by introducing a bisecting N-acetylglucosamine residue. In this study we investigated the effect of GnT-III on epidermal growth factor (EGF) signaling in HeLaS3 cells. Although the binding of EGF to the epidermal growth factor receptor (EGFR) was ... More
Labeling of antibodies by in situ modification of thiol groups generated from selenol-catalyzed reduction of native disulfide bonds.
AuthorsSingh R, Maloney EK
JournalAnal Biochem
PubMed ID12009690
A new method for labeling antibodies which involves selenol-catalyzed reduction of native disulfide bonds in antibodies to generate thiol groups, which then are labeled using thiol-reactive reagents, is described. The reduction and labeling steps of this rapid procedure are carried out in one vessel, without requiring any separation step to ... More
Separation of early steps in endocytic membrane transport.
Authorsvan der Goot FG
JournalElectrophoresis
PubMed ID9527499
We describe a simple subcellular fractionation scheme aimed at separating early endosomes from the plasma membrane in view of studying the possible arrival of plasma membrane-bound toxins, proteins or other extracellular ligands in endosomes. Plasma membrane proteins were labeled with the impermeable reagent sulfosuccinimidyl-6-(biotinamido)hexanoate (NHS-LC) biotin at 4 degrees C. ... More
In vivo interaction of the adapter protein CD2-associated protein with the type 2 polycystic kidney disease protein, polycystin-2.
AuthorsLehtonen S, Ora A, Olkkonen VM, Geng L, Zerial M, Somlo S, Lehtonen E
JournalJ Biol Chem
PubMed ID10913159
We identified a developmentally regulated gene from mouse kidney whose expression is up-regulated in metanephrogenic mesenchyme cells when they are induced to differentiate to epithelial cells during kidney organogenesis. The deduced 70.5-kDa protein, originally named METS-1 (mesenchyme-to-epithelium transition protein with SH3 domains), has since been cloned as a CD2-associated protein ... More
MHC class I-related neonatal Fc receptor for IgG is functionally expressed in monocytes, intestinal macrophages, and dendritic cells.
AuthorsZhu X, Meng G, Dickinson BL, Li X, Mizoguchi E, Miao L, Wang Y, Robert C, Wu B, Smith PD, Lencer WI, Blumberg RS
JournalJ Immunol
PubMed ID11207281
The neonatal Fc receptor (FcRn) for IgG, an MHC class I-related molecule, functions to transport IgG across polarized epithelial cells and protect IgG from degradation. However, little is known about whether FcRn is functionally expressed in immune cells. We show here that FcRn mRNA was identifiable in human monocytes, macrophages, ... More
CD63 associates with tyrosine kinase activity and CD11/CD18, and transmits an activation signal in neutrophils.
AuthorsSkubitz KM, Campbell KD, Iida J, Skubitz AP
JournalJ Immunol
PubMed ID8871662
As a member of the tetraspan family, it has been hypothesized that CD63 may be associated with signal transduction; however, its role in leukocyte function is unknown. To examine the potential ability of CD63 to activate neutrophils, the effects of five CD63 mAbs, AHN-16, -16.1, -16.2, -16.3, and -16.5, were ... More
A cell-free assay for glycosylphosphatidylinositol anchoring in African trypanosomes. Demonstration of a transamidation reaction mechanism.
AuthorsSharma DK, Vidugiriene J, Bangs JD, Menon AK
JournalJ Biol Chem
PubMed ID10347210
We established an in vitro assay for the addition of glycosyl-phosphatidylinositol (GPI) anchors to proteins using procyclic trypanosomes engineered to express GPI-anchored variant surface glycoprotein (VSG). The assay is based on the premise that small nucleophiles, such as hydrazine, can substitute for the GPI moiety and effect displacement of the ... More
A dominant-negative clathrin mutant differentially affects trafficking of molecules with distinct sorting motifs in the class II major histocompatibility complex (MHC) pathway.
AuthorsLiu SH, Marks MS, Brodsky FM
JournalJ Cell Biol
PubMed ID9490717
The role of clathrin in intracellular sorting was investigated by expression of a dominant-negative mutant form of clathrin, termed the hub fragment. Hub inhibition of clathrin-mediated membrane transport was established by demonstrating a block of transferrin internalization and an alteration in the intracellular distribution of the cation-independent mannose-6-phosphate receptor. Hubs ... More
Cystic fibrosis transmembrane conductance regulator: the first nucleotide binding fold targets the membrane with retention of its ATP binding function.
AuthorsKo YH, Delannoy M, Pedersen PL
JournalBiochemistry
PubMed ID9125527
Most cases of cystic fibrosis are caused by a single deletion mutation (deltaF508) within the first nucleotide binding fold (NBF1) of the CFTR protein (cystic fibrosis transmembrane conductance regulator). NBF1 is classically defined as amino acid residues phenylalanine 433 through serine 589, encoded by exons 10-12, and only part of ... More
The Ca2+ dependence of human Fc gamma receptor-initiated phagocytosis.
AuthorsEdberg JC, Lin CT, Lau D, Unkeless JC, Kimberly RP
JournalJ Biol Chem
PubMed ID7673212
Differing roles for [Ca2+]i transients in Fc gamma R-mediated phagocytosis have been suggested based on the observations that antibody-opsonized erythrocyte phagocytosis by human neutrophils shows a [Ca2+]i dependence, while that by murine macrophages appears [Ca2+]i-independent. To explore whether this difference might reflect different receptor isoforms or different cell types, we ... More
Localization and recycling of gp27 (hp24gamma3): complex formation with other p24 family members.
AuthorsFüllekrug J, Suganuma T, Tang BL, Hong W, Storrie B, Nilsson T
JournalMol Biol Cell
PubMed ID10359607
We report here the characterization of gp27 (hp24gamma3), a glycoprotein of the p24 family of small and abundant transmembrane proteins of the secretory pathway. Immunoelectron and confocal scanning microscopy show that at steady state, gp27 localizes to the cis side of the Golgi apparatus. In addition, some gp27 was detected ... More
Turnover analysis of glutamate receptors identifies a rapidly degraded pool of the N-methyl-D-aspartate receptor subunit, NR1, in cultured cerebellar granule cells.
AuthorsHuh KH, Wenthold RJ
JournalJ Biol Chem
PubMed ID9867823
The number, composition, and location of receptors in neurons are critically important factors in determining the neuron's response to neurotransmitters. The functional expression of receptors appears to be regulated both generally, at the level of transcription or translation, and locally, at the level of the individual synapse. A key component ... More
The A-domain of beta 2 integrin CR3 (CD11b/CD18) is a receptor for the hookworm-derived neutrophil adhesion inhibitor NIF.
AuthorsRieu P, Ueda T, Haruta I, Sharma CP, Arnaout MA
JournalJ Cell Biol
PubMed ID7528750
The A-domain is a approximately 200-amino acid peptide present within structurally diverse proadhesive proteins including seven integrins. A recombinant form of the A-domain of beta 2 integrins CR3 and LFA-1 has been recently shown to bind divalent cations and to contain binding sites for protein ligands that play essential roles ... More
Synaptic-like microvesicles of neuroendocrine cells originate from a novel compartment that is continuous with the plasma membrane and devoid of transferrin receptor.
AuthorsSchmidt A, Hannah MJ, Huttner WB
JournalJ Cell Biol
PubMed ID9128254
We have characterized the compartment from which synaptic-like microvesicles (SLMVs), the neuroendocrine counterpart of neuronal synaptic vesicles, originate. For this purpose we have exploited the previous observation that newly synthesized synaptophysin, a membrane marker of synaptic vesicles and SLMVs, is delivered to the latter organelles via the plasma membrane and ... More
TGF-beta induced transdifferentiation of mammary epithelial cells to mesenchymal cells: involvement of type I receptors.
AuthorsMiettinen PJ, Ebner R, Lopez AR, Derynck R
JournalJ Cell Biol
PubMed ID7806579
The secreted polypeptide transforming growth factor-beta (TGF-beta) exerts its multiple activities through type I and II cell surface receptors. In epithelial cells, activation of the TGF-beta signal transduction pathways leads to inhibition of cell proliferation and an increase in extracellular matrix production. TGF-beta is widely expressed during development and its ... More
Biotinylation of proteins via amino groups can induce binding to U937 cells, HL-60 cells, monocytes and granulocytes.
AuthorsStorm D, Loos M, Kaul M
JournalJ Immunol Methods
PubMed ID8960102
The use of biotinylated ligands for the flow cytometric detection of cell surface receptors has become a popular alternative to radioreceptor assays. Although the biotinylation of a protein is a relatively mild chemical reaction several reports have mentioned the fact that the number and location of biotin moieties coupled to ... More
gC1q-R/p33, a member of a new class of multifunctional and multicompartmental cellular proteins, is involved in inflammation and infection.
AuthorsGhebrehiwet B, Lim BL, Kumar R, Feng X, Peerschke EI
JournalImmunol Rev
PubMed ID11414365
Human gC1q-R (p33, p32, C1qBP, TAP) is a ubiquitously expressed, multiligand-binding, multicompartmental cellular protein involved in various ligand-mediated cellular responses. Although expressed on the surface of cells, an intriguing feature of the membrane-associated form of gC1q-R is that its translated amino acid sequence does not predict the presence of either ... More
Fiber-optic evanescent wave biosensor for the detection of oligonucleotides.
AuthorsAbel AP, Weller MG, Duveneck GL, Ehrat M, Widmer HM
JournalAnal Chem
PubMed ID8794925
An automated optical biosensor system based on fluorescence excitation and detection in the evanescent field of a quartz fiber was used to detect 16-mer oligonucleotides in DNA hybridization assays. A biotinylated capture probe was immobilized on the fiber surface via avidin or streptavidin. The hybridization with fluorescein-labeled complementary strands was ... More
Dictyostelium myosin I double mutants exhibit conditional defects in pinocytosis.
AuthorsNovak KD, Peterson MD, Reedy MC, Titus MA
JournalJ Cell Biol
PubMed ID8522584
The functional relationship between three Dictyostelium myosin Is, myoA, myoB, and myoC, has been examined through the creation of double mutants. Two double mutants, myoA-/B- and myoB-/C-, exhibit similar conditional defects in fluid-phase pinocytosis. Double mutants grown in suspension culture are significantly impaired in their ability to take in nutrients ... More
Preservation of NADH voltammetry for enzyme-modified electrodes based on dehydrogenase.
AuthorsHayes MA, Kuhr WG
JournalAnal Chem
PubMed ID10330904
Minimizing overpotential and generating high faradaic currents are critical issues for fast-scan voltammetry of beta-nicotinamide adenine dinucleotide (NADH) for the sensitivity of enzyme-modified electrodes based on dehydrogenases. Although NADH voltammetry exhibits high overpotential and poor voltammetric peak shape at solid electrode surfaces, modification of the electrode surface can improve the ... More
Phagosomes acquire nascent and recycling class II MHC molecules but primarily use nascent molecules in phagocytic antigen processing.
AuthorsRamachandra L, Harding CV
JournalJ Immunol
PubMed ID10799867
Phagosomes contain class II MHC (MHC-II) and form peptide:MHC-II complexes, but the source of phagosomal MHC-II molecules is uncertain. Phagosomes may acquire nascent MHC-II or preexisting, recycling MHC-II that may be internalized from the plasma membrane. Brefeldin A (BFA) was used to deplete nascent MHC-II in murine macrophages to determine ... More
The occurrence of O-acylation during biotinylation of gonadotropin-releasing hormone and analogs. Evidence for a reactive serine.
AuthorsMiller BT, Collins TJ, Nagle GT, Kurosky A
JournalJ Biol Chem
PubMed ID1544890
Gonadotropin-releasing hormone (GnRH) and two of its analogs ([D-Lys6]GnRH and des-Gly10-[D-Trp6]-GnRH) were reacted with sulfonated N-hydroxysuccinimide esters of biotin that have been reported to react specifically with primary amino groups. Fractionation by reversed-phase high performance liquid chromatography demonstrated the occurrence of multiple biotinylated derivatives for each reacted peptide. These results ... More
A biomimetic pH-responsive polymer directs endosomal release and intracellular delivery of an endocytosed antibody complex.
AuthorsLackey CA, Press OW, Hoffman AS, Stayton PS
JournalBioconjug Chem
PubMed ID12236781
Poly(propylacrylic acid) (PPAAc) is a synthetic pH-responsive polymer that has been shown to disrupt cell membranes at low pH values typical of the endosome, but not at physiological pH, suggesting its use as an endosomal-releasing agent [Murthy et al. J. Controlled Release 61, 137-43]. We have constructed an antibody-targeted biotherapeutic ... More
The cytosolic termini of the beta- and gamma-ENaC subunits are involved in the functional interactions between cystic fibrosis transmembrane conductance regulator and epithelial sodium channel.
AuthorsJi HL, Chalfant ML, Jovov B, Lockhart JP, Parker SB, Fuller CM, Stanton BA, Benos DJ
JournalJ Biol Chem
PubMed ID10821834
Epithelial sodium channel (ENaC) and cystic fibrosis transmembrane conductance regulator (CFTR) are co-localized in the apical membrane of many epithelia. These channels are essential for electrolyte and water secretion and/or reabsorption. In cystic fibrosis airway epithelia, a hyperactivated epithelial Na(+) conductance operates in parallel with defective Cl(-) secretion. Several groups ... More
The human AC133 hematopoietic stem cell antigen is also expressed in epithelial cells and targeted to plasma membrane protrusions.
AuthorsCorbeil D, Röper K, Hellwig A, Tavian M, Miraglia S, Watt SM, Simmons PJ, Peault B, Buck DW, Huttner WB
JournalJ Biol Chem
PubMed ID10681530
The human AC133 antigen and mouse prominin are structurally related plasma membrane proteins. However, their tissue distribution is distinct, with the AC133 antigen being found on hematopoietic stem and progenitor cells and prominin on various epithelial cells. To determine whether the human AC133 antigen and mouse prominin are orthologues or ... More
Phorbol ester induces the rapid processing of cell surface heparin-binding EGF-like growth factor: conversion from juxtacrine to paracrine growth factor activity.
AuthorsGoishi K, Higashiyama S, Klagsbrun M, Nakano N, Umata T, Ishikawa M, Mekada E, Taniguchi N
JournalMol Biol Cell
PubMed ID7579712
Vero cell heparin-binding epidermal growth factor-like growth factor (HB-EGF) is synthesized as a 20- to 30-kDa membrane-anchored HB-EGF precursor (proHB-EGF). Localization and processing of proHB-EGF, both constitutive and 12-O-tetradecanoylphorbol 13-acetate (TPA)-inducible, was examined in Vero cells overexpressing recombinant HB-EGF (Vero H cells). Flow cytometry and fluorescence immunostaining demonstrated that Vero ... More
The proamphiregulin cytoplasmic domain is required for basolateral sorting, but is not essential for constitutive or stimulus-induced processing in polarized Madin-Darby canine kidney cells.
AuthorsBrown CL, Coffey RJ, Dempsey PJ
JournalJ Biol Chem
PubMed ID11382759
In this study, the role of the amphiregulin precursor (pro-AR) cytoplasmic domain in the basolateral sorting and cell-surface processing of pro-AR in polarized epithelial cells has been investigated using Madin-Darby canine kidney cells stably expressing various human pro-AR forms. Our results demonstrate that newly synthesized wild-type pro-AR (50 kDa) is ... More
Ii chain controls the transport of major histocompatibility complex class II molecules to and from lysosomes.
AuthorsBrachet V, Raposo G, Amigorena S, Mellman I
JournalJ Cell Biol
PubMed ID9105036
Major histocompatibility complex class II molecules are synthesized as a nonameric complex consisting of three alpha beta dimers associated with a trimer of invariant (Ii) chains. After exiting the TGN, a targeting signal in the Ii chain cytoplasmic domain directs the complex to endosomes where Ii chain is proteolytically processed ... More
The Chlamydomonas mating type plus fertilization tubule, a prototypic cell fusion organelle: isolation, characterization, and in vitro adhesion to mating type minus gametes.
AuthorsWilson NF, Foglesong MJ, Snell WJ
JournalJ Cell Biol
PubMed ID9199169
In the biflagellated alga Chlamydomonas, adhesion and fusion of the plasma membranes of gametes during fertilization occurs via an actin-filled, microvillus-like cell protrusion. Formation of this approximately 3-microm-long fusion organelle, the Chlamydomonas fertilization tubule, is induced in mating type plus (mt+) gametes during flagellar adhesion with mating type minus (mt-) ... More