Rescue of vasopressin V2 receptor mutants by chemical chaperones: specificity and mechanism.
AuthorsRobben JH,Sze M,Knoers NV,Deen PM
JournalMolecular biology of the cell
PubMed ID16267275
Because missense mutations in genetic diseases of membrane proteins often result in endoplasmic reticulum (ER) retention of functional proteins, drug-induced rescue of their cell surface expression and understanding the underlying mechanism are of clinical value. To study this, we tested chemical chaperones and sarco(endo)plasmic reticulum Ca(2+) ATPase pump inhibitors on ... More
The small GTP-binding protein Rac promotes the dissociation of gelsolin from actin filaments in neutrophils.
AuthorsArcaro A
JournalJ Biol Chem
PubMed ID9422735
Gelsolin is an actin filament-capping protein that has been shown to play a key role in cell migration. Here we have studied the involvement of phosphoinositide 3-kinase (PI 3-kinase) and GTP-binding proteins (G-proteins) in the regulation of gelsolin-actin interactions in neutrophils. Inhibition of PI 3-kinase activity in vivo by wortmannin ... More
Isoform-specific up-regulation by ouabain of Na+,K+-ATPase in cultured rat astrocytes.
AuthorsHosoi R, Matsuda T, Asano S, Nakamura H, Hashimoto H, Takuma K, Baba A
JournalJ Neurochem
PubMed ID9349566
There are two alpha-subunit isoforms (alpha1 and alpha2) and two beta-subunit isoforms (beta1 and beta2) of Na+,K+-ATPase in astrocytes, but the functional heterodimer composition is not known. Ouabain (0.5-1.0 mM) increased the levels of alpha1 and beta1 mRNAs, whereas it decreased those of alpha2 and beta2 mRNAs in cultured rat ... More
Degradation of lamin B1 precedes oligonucleosomal DNA fragmentation in apoptotic thymocytes and isolated thymocyte nuclei.
AuthorsNeamati N, Fernandez A, Wright S, Kiefer J, McConkey DJ
JournalJ Immunol
PubMed ID7535814
Chromatin condensation and nuclear envelope breakdown are characteristic features of apoptotic cell death, but the mechanisms underlying these phenomena have not been identified. Solubilization of nuclear lamin is responsible for both events in mitosis. In this work, we report that glucocorticoids stimulate rapid degradation of lamin B1 that occurs before ... More
Calcineurin feedback inhibition of agonist-evoked cAMP formation.
AuthorsAntoni FA, Barnard RJ, Shipston MJ, Smith SM, Simpson J, Paterson JM
JournalJ Biol Chem
PubMed ID7499291
The effects of immunosuppressant blockers of calcineurin (protein phosphatase 2B) on cAMP formation and hormone release were investigated in mouse pituitary tumor (AtT20) cells. Immunosuppressants enhanced corticotropin-releasing factor- and isoproterenol-evoked cAMP production in proportion with their potency to block calcineurin. Further analysis of cAMP production revealed that intracellular Ca2+ derived ... More
NMDA receptor activation in differentiating cerebellar cell cultures regulates the expression of a new POU gene, Cns-1.
AuthorsBulleit RF, Cui H, Wang J, Lin X
JournalJ Neurosci
PubMed ID7907365
POU/homeobox genes encode transcription regulatory proteins that are important in defining cellular phenotypes. Expression of these genes may be critical for to the regulation of CNS cellular differentiation. We have identified a cDNA corresponding to a new member of the POU/homeobox gene family. Expression of RNA encoded by this new ... More
Detection of ligand-activated conductive Ca2+ channels in human B lymphocytes.
AuthorsMacDougall SL, Grinstein S, Gelfand EW
JournalCell
PubMed ID2455602
It has been assumed that uptake of extracellular Ca2+ occurs through ligand-activated Ca2+ channels in anti-IgM stimulated human B cells. If so, then uptake should be associated with a depolarizing inward current. Instead, a hyperpolarization due to Ca2+-sensitive K+ conductance is observed. To demonstrate conductive Ca2+ channels in human B ... More
Mechanism of collagen activation in human platelets.
AuthorsRoberts DE, McNicol A, Bose R
JournalJ Biol Chem
PubMed ID14981087
The mechanism of collagen-induced human platelet activation was examined using Ca2+, Na+, and the pH-sensitive fluorescent dyes calcium green/fura red, sodium-binding benzofuran isophthalate, and 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Administration of a moderate dose of collagen (10 microg/ml) to human platelets resulted in an increase in [Ca2+](i) and platelet aggregation. The majority of this ... More
Identification of band 3-like proteins and Cl-/HCO3- exchange in isolated cardiomyocytes.
AuthorsPucéat M, Korichneva I, Cassoly R, Vassort G
JournalJ Biol Chem
PubMed ID7836397
'The identification of the protein that exerts the function of Cl-/HCO3- exchange is still unresolved in cardiac tissue. We have addressed this issue by using a multiple technical approach. Western blotting analysis with an antibody raised against human erythroid whole band 3 protein, the so-called protein that mediates the Cl-/HCO3- ... More
Regulation of a calcium-dependent tyrosine kinase in vascular smooth muscle cells by angiotensin II and platelet-derived growth factor. Dependence on calcium and the actin cytoskeleton.
AuthorsBrinson AE, Harding T, Diliberto PA, He Y, Li X, Hunter D, Herman B, Earp HS, Graves LM
JournalJ Biol Chem
PubMed ID9430717
'A novel, p125FAK homologue, CADTK, has been detected in neural, epithelial, or hematopoietic cells but not in fibroblasts. We now demonstrate CADTK expression in a mesenchymal cell, rat aortic smooth muscle cells (RSMC). Angiotensin II (Ang II) or platelet-derived growth factor (PDGF-BB and PDGF-AA) markedly stimulated CADTK tyrosine phosphorylation in ... More
A role for calcium in stabilizing transport vesicle coats.
Authors Ahluwalia J P; Topp J D; Weirather K; Zimmerman M; Stamnes M;
JournalJ Biol Chem
PubMed ID11435443
'Calcium has been implicated in regulating vesicle fusion reactions, but its potential role in regulating other aspects of protein transport, such as vesicle assembly, is largely unexplored. We find that treating cells with the membrane-permeable calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N'',N''-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), leads to a dramatic redistribution of the vesicle ... More
Focal exocytosis of VAMP3-containing vesicles at sites of phagosome formation.
AuthorsBajno L, Peng XR, Schreiber AD, Moore HP, Trimble WS, Grinstein S
JournalJ Cell Biol
PubMed ID10791982
'Phagocytosis involves the receptor-mediated extension of plasmalemmal protrusions, called pseudopods, which fuse at their tip to engulf a particle. Actin polymerizes under the nascent phagosome and may propel the protrusion of pseudopods. Alternatively, membrane extension could result from the localized insertion of intracellular membranes into the plasmalemma next to the ... More
Galpha11 induces caspase-mediated proteolytic activation of Rho-associated kinase, ROCK-I, in HeLa cells.
'Expression of the constitutively active mutant of Galpha(11) (Galpha(11)QL) induces the formation of vinculin-containing focal adhesion-like structures in HeLa cells. This was found to be inhibited by Y-27632, a specific inhibitor of Rho-associated kinases (ROCK), but not by co-expression with a dominant negative mutant of RhoA, suggesting Rho-independent activation of ... More
The platelet cytoskeleton regulates the affinity of the integrin alpha(IIb)beta(3) for fibrinogen.
AuthorsBennett JS, Zigmond S, Vilaire G, Cunningham ME, Bednar B
JournalJ Biol Chem
PubMed ID10464255
'Agonist-generated inside-out signals enable the platelet integrin alpha(IIb)beta(3) to bind soluble ligands such as fibrinogen. We found that inhibiting actin polymerization in unstimulated platelets with cytochalasin D or latrunculin A mimics the effects of platelet agonists by inducing fibrinogen binding to alpha(IIb)beta(3). By contrast, stabilizing actin filaments with jasplakinolide prevented ... More
Cadherins mediate intercellular mechanical signaling in fibroblasts by activation of stretch-sensitive calcium-permeable channels.
AuthorsKo KS, Arora PD, McCulloch CA
JournalJ Biol Chem
PubMed ID11466312
'Cells in mechanically active environments form extensive, cadherin-mediated intercellular junctions that are important in tissue remodeling and differentiation. Currently, it is unknown whether adherens junctions in connective tissue fibroblasts transmit mechanical signals and coordinate multicellular adaptations to physical forces. We hypothesized that cadherins mediate intercellular mechanotransduction by activating calcium-permeable, stretch-sensitive ... More
Acetylcholine-induced calcium signaling and contraction of airway smooth muscle cells in lung slices.
AuthorsBergner A, Sanderson MJ
JournalJ Gen Physiol
PubMed ID11815668
'The Ca(2+) signaling and contractility of airway smooth muscle cells (SMCs) were investigated with confocal microscopy in murine lung slices (approximately 75-microm thick) that maintained the in situ organization of the airways and the contractility of the SMCs for at least 5 d. 10--500 nM acetylcholine (ACH) induced a contraction ... More
The role of actin-binding protein 280 in integrin-dependent mechanoprotection.
AuthorsGlogauer M, Arora P, Chou D, Janmey PA, Downey GP, McCulloch CA
JournalJ Biol Chem
PubMed ID9430714
'To survive in a mechanically active environment, cells must adapt to variations of applied membrane tension. A collagen-coated magnetic bead model was used to apply forces directly to the actin cytoskeleton through integrin receptors. We demonstrate here that by a calcium-dependent mechanism, human fibroblasts reinforce locally their connection with extracellular ... More
Na+-dependent release of Mg2+ from an intracellular pool in rat sublingual mucous acini.
AuthorsZhang GH, Melvin JE
JournalJ Biol Chem
PubMed ID8910560
'Muscarinic stimulation induces release of Mg2+ from an intracellular pool in rat sublingual mucous acini (Zhang, G. H., and Melvin, J. E. (1992) J. Biol. Chem. 267, 20721-20727). In the present study we examined the interdependence of Mg2+ mobilization on intracellular Na+ and Ca2+ by monitoring the intracellular free concentrations ... More
Cadmium-induced expression of immediate early genes in LLC-PK1 cells.
AuthorsMatsuoka M, Call KM
JournalKidney Int
PubMed ID7564105
'To identify molecular mechanisms underlying renal cell damage by cadmium, the effect of this heavy metal on the level of immediate early genes (IEGs) transcripts in LLC-PK1 cells was studied. Cadmium chloride (CdCl2) induced the expression of four IEGs examined, but with differing time courses. The level of c-fos mRNA ... More
The polycystic kidney disease 1 gene product mediates protein kinase C alpha-dependent and c-Jun N-terminal kinase-dependent activation of the transcription factor AP-1.
AuthorsArnould T, Kim E, Tsiokas L, Jochimsen F, Grüning W, Chang JD, Walz G
JournalJ Biol Chem
PubMed ID9497315
'Autosomal dominant polycystic kidney disease (ADPKD) is a common hereditary disorder that accounts for 8-10% of end stage renal disease. PKD1, one of two recently isolated ADPKD gene products, has been implicated in cell-cell and cell-matrix interactions. However, the signaling pathway of PKD1 remains undefined. We found that the C-terminal ... More
Reactive oxygen species (ROS)-induced ROS release: a new phenomenon accompanying induction of the mitochondrial permeability transition in cardiac myocytes.
'We sought to understand the relationship between reactive oxygen species (ROS) and the mitochondrial permeability transition (MPT) in cardiac myocytes based on the observation of increased ROS production at sites of spontaneously deenergized mitochondria. We devised a new model enabling incremental ROS accumulation in individual mitochondria in isolated cardiac myocytes ... More
The cytoplasmic domain of human FcgammaRIa alters the functional properties of the FcgammaRI.gamma-chain receptor complex.
'The gamma/zeta-chain family of proteins mediate cell activation for multiple immunoglobulin receptors. However, the recognition that these receptors may have distinct biologic functions suggests that additional signaling elements may contribute to functional diversity. We hypothesized that the cytoplasmic domain (CY) of the ligand binding alpha-chain alters the biological properties of ... More
Native LDL potentiate TNF alpha and IL-8 production by human mononuclear cells.
AuthorsNetea MG, Kullberg BJ, Demacker PN, Jacobs LE, Verver-Jansen TJ, Hijmans A, van Tits LH, Hoenderop JG, Willems PH, Van der Meer JW, Stalenhoef AF
JournalJ Lipid Res
PubMed ID12091490
'Native LDL (nLDL) increases expression of adhesion molecules on endothelial cells through induction of Ca(2+) mobilization. Ca(2+) mobilization is also involved in the induction of proinflammatory cytokines, important mediators involved in atherogenesis. The aim of the study was to evaluate the capacity of nLDL to affect spontaneous and lipopolysaccharide (LPS)-stimulated ... More
fMet-Leu-Phe-induced activation of phospholipase D in human neutrophils. Dependence on changes in cytosolic free Ca2+ concentration and relation with respiratory burst activation.
AuthorsKessels GC, Roos D, Verhoeven AJ
JournalJ Biol Chem
PubMed ID1744114
'In this study, we have investigated the Ca2+ requirements for the activation of phospholipase D by the tripeptide fMet-Leu-Phe (fMLP) in human neutrophils. EGTA inhibited the activation of phospholipase D (PLD) by 55% (n = 4). When the initial transient rise in [Ca2+]i was prevented by loading the cells with ... More
Activation of the respiratory burst in human eosinophils by chemotaxins requires intracellular calcium fluxes.
AuthorsElsner J, Dichmann S, Kapp A
JournalJ Invest Dermatol
PubMed ID7636306
'Eosinophils represent major effector cells in the allergic inflammatory response. Following activation, these cells are capable of mediating tissue damage, particularly by the release of reactive oxygen species. In this study, the role of extracellular and intracellular calcium in the induction of the respiratory burst of human eosinophils was investigated ... More
Membrane potential modulates release of tumor necrosis factor in lipopolysaccharide-stimulated mouse macrophages.
AuthorsHaslberger A, Romanin C, Koerber R
JournalMol Biol Cell
PubMed ID1498365
'Lipopolysaccharide (LPS)-mediated synthesis of macrophage gene products such as tumor necrosis factor (TNF) is controlled by different signaling pathways. We investigated intracellular free Ca2+ (Ca2+ic) and the membrane potential as early cellular responses to LPS and their role in the synthesis and release of TNF. In peritoneal macrophages and in ... More
Fc gamma II receptor-mediated platelet activation induced by anti-CD9 monoclonal antibody opens Ca2+ channels which are distinct from those associated with Ca2+ store depletion.
AuthorsKuroda K, Ozaki Y, Qi R, Asazuma N, Yatomi Y, Satoh K, Nomura S, Suzuki M, Kume S
JournalJ Immunol
PubMed ID7594604
'Anti-human platelet CD9 mAb, NNKY1-19, induced platelet activation in a Fc gamma RII-dependent manner in terms of aggregation and secretion of intracellular granule contents. These responses were considerably suppressed by aspirin. [Ca2+]i elevation in the absence of extracellular Ca2+ ([Ca2+]e), which represents the amount of Ca2+ released from intracellular Ca2+ ... More
Amyloid beta-peptide induces cell monolayer albumin permeability, impairs glucose transport, and induces apoptosis in vascular endothelial cells.
AuthorsBlanc EM, Toborek M, Mark RJ, Hennig B, Mattson MP
JournalJ Neurochem
PubMed ID9109512
'Amyloid beta-peptide (A beta) is deposited as insoluble fibrils in the brain parenchyma and cerebral blood vessels in Alzheimer''s disease (AD). In addition to neuronal degeneration, cerebral vascular alterations indicative of damage to vascular endothelial cells and disruption of the blood-brain barrier occur in AD. Here we report that A ... More
Related adhesion focal tyrosine kinase and the epidermal growth factor receptor mediate the stimulation of mitogen-activated protein kinase by the G-protein-coupled P2Y2 receptor. Phorbol ester or [Ca2+]i elevation can substitute for receptor activation.
AuthorsSoltoff SP
JournalJ Biol Chem
PubMed ID9722539
'The activation of growth factor receptors and receptors coupled to heterotrimeric guanine nucleotide-binding proteins (G-proteins) can increase mitogen-activated protein (MAP) kinase activity in many cells. Previously, we demonstrated that the activation of G-protein-coupled P2Y2 receptors by extracellular ATP and UTP stimulated MAP (p42 ERK2) kinase by a mechanism that was ... More
Paxillin is tyrosine-phosphorylated by and preferentially associates with the calcium-dependent tyrosine kinase in rat liver epithelial cells.
AuthorsLi X, Earp HS
JournalJ Biol Chem
PubMed ID9162070
'We and others have recently cloned a non-receptor, calcium-dependent tyrosine kinase (CADTK; also known as PYK2, CAKbeta, and RAFTK) that shares both overall domain structure and 45% amino acid identity with p125(FAK). We have studied the signaling, activation, and potential function of these related enzymes in GN4 rat liver epithelial ... More
ATP activates DNA synthesis by acting on P2X receptors in human osteoblast-like MG-63 cells.
AuthorsNakamura E, Uezono Y, Narusawa K, Shibuya I, Oishi Y, Tanaka M, Yanagihara N, Nakamura T, Izumi F
JournalAm J Physiol Cell Physiol
PubMed ID10913018
'In human osteoblast-like MG-63 cells, extracellular ATP increased [(3)H]thymidine incorporation and cell proliferation and synergistically enhanced platelet-derived growth factor- or insulin-like growth factor I-induced [(3)H]thymidine incorporation. ATP-induced [(3)H]thymidine incorporation was mimicked by the nonhydrolyzable ATP analogs adenosine 5''-O-(3-thiotriphosphate) and adenosine 5''-adenylylimidodiphosphate and was inhibited by the P2 purinoceptor antagonist suramin, ... More
Ca2+ influx mediates apoptosis induced by 4-aminopyridine, a K+ channel blocker, in HepG2 human hepatoblastoma cells.
AuthorsKim JA, Kang YS, Jung MW, Kang GH, Lee SH, Lee YS
JournalPharmacology
PubMed ID10657756
'Apoptosis appears to be implicated in the pathogenesis and therapeutic applications of cancer. In this study we investigated the induction of apoptosis by 4-aminopyridine (4-AP), a K(+) channel blocker, and its mechanism in HepG2 human hepatoblastoma cells. 4-AP reduced cell viability and induced DNA fragmentation, a hallmark of apoptosis, in ... More
Phosphorylation and regulation of antidepressant-sensitive serotonin transporters.
AuthorsRamamoorthy S, Giovanetti E, Qian Y, Blakely RD
JournalJ Biol Chem
PubMed ID9442097
'Antidepressant-sensitive serotonin (5-hydroxytryptamine, 5HT) transporters (SERTs) are responsible for efficient synaptic clearance of extracellular 5HT. Previously (Qian, Y., Galli, A., Ramamoorthy, S., Risso, S., DeFelice, L. J., and Blakely, R. D. (1997) J. Neurosci. 17, 45-47), we demonstrated that protein kinase (PKC)-linked pathways in transfected HEK-293 cells lead to the ... More
Control of IP(3)-mediated Ca2+ puffs in Xenopus laevis oocytes by the Ca2+-binding protein parvalbumin.
AuthorsJohn LM, Mosquera-Caro M, Camacho P, Lechleiter JD
JournalJ Physiol
PubMed ID11507154
'1. Elementary events of Ca2+ release (Ca2+ puffs) can be elicited from discrete clusters of inositol 1,4,5 trisphosphate receptors (IP(3)Rs) at low concentrations of IP(3). Ca(2+) puffs have rarely been observed unless elicited by either hormone treatment or introduction of IP(3) into the cell. However, cells appear to have sufficient ... More
Chemoattractant-induced cytoplasmic pH changes and cytoskeletal reorganization in human neutrophils. Relationship to the stimulated calcium transients and oxidative burst.
AuthorsNaccache PH, Therrien S, Caon AC, Liao N, Gilbert C, McColl SR
JournalJ Immunol
PubMed ID2538509
'The relationships between the chemotactic factor-stimulated mobilization of calcium, activation of the NADPH-oxidase, changes in cytosolic pH, and in the level of polymerized actin in human neutrophils have been examined. The approach taken was to use intracellular calcium chelators, and pharmacologic modulators (both positive and negative) of the NADPH-oxidase to ... More
Regulatory volume decrease and intracellular Ca2+ in murine neuroblastoma cells studied with fluorescent probes.
'The possible role of Ca2+ as a second messenger mediating regulatory volume decrease (RVD) in osmotically swollen cells was investigated in murine neural cell lines (N1E-115 and NG108-15) by means of novel microspectrofluorimetric techniques that allow simultaneous measurement of changes in cell water volume and [Ca2+]i in single cells loaded ... More
Characterization of regulatory volume decrease in the THP-1 and HL-60 human myelocytic cell lines.
AuthorsGallin EK, Mason TM, Moran A
JournalJ Cell Physiol
PubMed ID7514614
'Exposure to hypotonic stress produces a transient increase in cell volume followed by a regulatory volume decrease (RVD) in both THP-1 and HL-60 cells. In contrast, cells exposed to hypotonic stress in a high K/low Na Hanks'' solution not only failed to volume regulate, but displayed a secondary swelling. Thus, ... More
Angiotensin II stimulates tyrosine phosphorylation of the focal adhesion-associated protein paxillin in aortic smooth muscle cells.
AuthorsLeduc I, Meloche S
JournalJ Biol Chem
PubMed ID7876204
'Treatment of vascular smooth muscle cells (SMC) with angiotensin II (AII) leads to an increase in the tyrosine phosphorylation of multiple cellular substrates. Here, we have demonstrated that AII stimulates tyrosine phosphorylation of the focal adhesion-associated protein paxillin in rat aortic SMC. AII-induced phosphorylation of paxillin was detectable within 1 ... More
Cell type-specific modes of feedback regulation of capacitative calcium entry.
AuthorsLouzao MC, Ribeiro CM, Bird GS, Putney JW
JournalJ Biol Chem
PubMed ID8662975
'The Ca2+-ATPase inhibitor, thapsigargin, activated Ca2+ entry into pancreatic acinar cells, a process known as capacitative calcium entry. In cells loaded with the calcium chelator BAPTA, the transient Ca2+ release was blunted and the rise of [Ca2+]i on readdition of Ca2+ was slowed. However, the steady-state [Ca2+]i due to Ca2+ ... More
HgCl2-induced interleukin-4 gene expression in T cells involves a protein kinase C-dependent calcium influx through L-type calcium channels.
AuthorsBadou A, Savignac M, Moreau M, Leclerc C, Pasquier R, Druet P, Pelletier L
JournalJ Biol Chem
PubMed ID9405450
'Mercuric chloride (HgCl2) induces T helper 2 (Th2) autoreactive anti-class II T cells in Brown Norway rats. These cells produce interleukin (IL)-4 and induce a B cell polyclonal activation that is responsible for autoimmune disease. In Brown Norway rats, HgCl2 triggers early IL-4 mRNA expression both in vivo and in ... More
Differential G protein-coupled cannabinoid receptor signaling by anandamide directs blastocyst activation for implantation.
AuthorsWang H, Matsumoto H, Guo Y, Paria BC, Roberts RL, Dey SK
JournalProc Natl Acad Sci U S A
PubMed ID14645706
'Mammalian fertility absolutely depends on synchronized development of the blastocyst to the stage when it is competent to implant, and the uterus to the stage when it is receptive to implantation. However, the molecular basis for the reciprocal interaction between the embryo and the uterus remains largely unexplored. One potentially ... More
Spontaneous quantal transmitter secretion from myocytes and fibroblasts: comparison with neuronal secretion.
AuthorsGirod R, Popov S, Alder J, Zheng JQ, Lohof A, Poo MM
JournalJ Neurosci
PubMed ID7722632
'When exogenous ACh is loaded into the cytoplasm of cultured amphibian myocytes and fibroblasts, the cells undergo spontaneous quantal ACh secretion, as detected by the appearance of pulsatile membrane currents in Xenopus myocytes which are manipulated into contact with the cells. These currents resemble in many ways the miniature endplate ... More
Increased internal Ca2+ mediates neural induction in the amphibian embryo.
AuthorsMoreau M, Leclerc C, Gualandris-Parisot L, Duprat AM
JournalProc Natl Acad Sci U S A
PubMed ID7809092
'The molecular mechanism of neural induction is still unknown and the identity of the natural inducer remains elusive. It has been suggested that both the protein kinase C and cAMP signal transduction pathways may be involved in mediating its action. Here we provide evidence that Ca2+ is implicated in the ... More
AuthorsOkuda M, Takahashi M, Suero J, Murry CE, Traub O, Kawakatsu H, Berk BC
JournalJ Biol Chem
PubMed ID10480886
'Fluid shear stress (flow) modulates endothelial cell function via specific intracellular signaling events. Previously we showed that flow activated ERK1/2 in an integrin-dependent manner (Takahashi, M., and Berk, B. C. (1996) J. Clin. Invest. 98, 2623-2631). p130 Crk-associated substrate (Cas), a putative c-Src substrate, was originally identified as a highly ... More
Regulation of L-type calcium channels in pituitary GH(4)C(1) cells by depolarization.
AuthorsPeri R, Triggle DJ, Singh S
JournalJ Biol Chem
PubMed ID11413135
'The neurosecretory anterior pituitary GH(4)C(1) cells exhibit the high voltage-activated dihydropyridine-sensitive L-type and the low voltage-activated T-type calcium currents. The activity of L-type calcium channels is tightly coupled to secretion of prolactin and other hormones in these cells. Depolarization induced by elevated extracellular K(+) reduces the dihydropyridine (+)-[(3)H]PN200-110 binding site ... More
Stretch-induced atriopeptin secretion in the isolated rat myocyte and its negative modulation by calcium.
AuthorsGreenwald JE, Apkon M, Hruska KA, Needleman P
JournalJ Clin Invest
PubMed ID2522102
'Cellular mechanism(s) regulating atriopeptin secretion and processing by the atrial myocyte are currently unknown. Osmotic stretch of isolated atrial myocytes as well as potassium chloride depolarization were potent stimuli of atriopeptin secretion. Release was potentiated by buffering either extracellular calcium with EGTA or intracellular calcium with the intracellular chelator, BAPTA ... More
Picomolar platelet-activating factor mobilizes Ca to change platelet shape without activating phospholipase C or protein kinase C; simultaneous fluorometric measurement of intracellular free Ca concentration and aggregation.
AuthorsJames-Kracke MR, Sexe RB, Shukla SD
JournalJ Pharmacol Exp Ther
PubMed ID7965802
'The purpose of this study was to investigate signal transduction mechanisms activated by low and high concentrations of platelet-activating factor (PAF) in rabbit platelets and to contrast the responses to those induced by thrombin. We measured changes in intracellular free calcium ([Ca++]i) with fura2, while monitoring light scatter simultaneously as ... More
Coassembly of Trp1 and Trp3 proteins generates diacylglycerol- and Ca2+-sensitive cation channels.
AuthorsLintschinger B, Balzer-Geldsetzer M, Baskaran T, Graier WF, Romanin C, Zhu MX, Groschner K
JournalJ Biol Chem
PubMed ID10882720
'To analyze the functional consequences of coassembly of transient receptor potential 1 (Trp1) and Trp3 channel proteins, we characterized membrane conductances and divalent cation entry derived by separate overexpression and by coexpression of both Trp isoforms. Trp1 expression generated a 1-oleoyl-2-acetyl-sn-glycerol (OAG)-activated conductance that was detectable only in Ca(2+)-free extracellular ... More
Identification of genes differentially expressed in V79 cells grown as multicell spheroids.
AuthorsOloumi A, Lam W, Banáth JP, Olive PL
JournalInt J Radiat Biol
PubMed ID12065053
'PURPOSE: Growth of Chinese hamster V79 cells as multicell spheroids leads to an increase in resistance to killing by ionizing radiation and etoposide. Differential display was used to identify changes in gene expression that occur when cells are grown as spheroids. MATERIALS AND METHODS: Differential display was performed using exponentially ... More
Calcium influx into neurons can solely account for cell contact-dependent neurite outgrowth stimulated by transfected L1.
'We have used monolayers of control 3T3 cells and 3T3 cells expressing transfected human L1 as a culture substrate for rat PC12 cells and rat cerebellar neurons. PC12 cells and cerebellar neurons extended longer neurites on human L1 expressing cells. Neurons isolated from the cerebellum at postnatal day 9 responded ... More
Mechanical strain induces monocyte chemotactic protein-1 gene expression in endothelial cells. Effects of mechanical strain on monocyte adhesion to endothelial cells.
AuthorsWang DL, Wung BS, Shyy YJ, Lin CF, Chao YJ, Usami S, Chien S
JournalCirc Res
PubMed ID7614716
'Monocyte chemotactic protein-1 (MCP-1), a potent monocyte chemoattractant secreted by endothelial cells (ECs), is believed to play a key role in the early events of atherogenesis. Since vascular ECs are constantly subjected to mechanical stresses, we examined how cyclic strain affects the expression of the MCP-1 gene in human ECs ... More
cAMP-induced secretion of endothelial von Willebrand factor is regulated by a phosphorylation/dephosphorylation switch in annexin A2.
AuthorsBrandherm I, Disse J, Zeuschner D, Gerke V,
Journal
PubMed ID23757730
'The large multimeric glyocoprotein von Willebrand factor (VWF) is a crucial component of both primary and secondary hemostasis. It is stored in secretory granules of vascular endothelial cells, the Weibel-Palade bodies (WPBs), and is released following stimulation by agonists that raise intracellular Ca(2+) or cyclic adenosine monophosphate (cAMP) levels. cAMP-induced ... More
Cytosolic free calcium elevation mediates the phagosome-lysosome fusion during phagocytosis in human neutrophils.
AuthorsJaconi ME, Lew DP, Carpentier JL, Magnusson KE, Sjögren M, Stendahl O
JournalJ Cell Biol
PubMed ID2110568
'Cytosolic free calcium ([Ca2+]i) and fusion of secondary granules with the phagosomal membrane (phagosome-lysosome fusion, P-L fusion) were assessed in single adherent human neutrophils during phagocytosis of C3bi-opsonized yeast particles. Neutrophils were loaded with the fluorescent dye fura2/AM and [Ca2+]i was assessed by dual excitation microfluorimetry. Discharge of lactoferrin, a ... More
Low frequency MFs increased inositol 1,4,5-trisphosphate levels in the Jurkat cell line.
AuthorsKorzh-Sleptsova IL, Lindström E, Mild KH, Berglund A, Lundgren E
JournalFEBS Lett
PubMed ID7867788
'We have earlier reported that when a weak 50 Hz MF (magnetic field) was applied, the leukemic T-cell line Jurkat responded with intracellular calcium oscillations [Lindström, et al., J. Cell Physiol., 156 (1993) 395-398]. The result suggested that the MF interfered with the signal transduction, although neither target molecules nor ... More
Exosome release is regulated by a calcium-dependent mechanism in K562 cells.
AuthorsSavina A, Furlán M, Vidal M, Colombo MI
JournalJ Biol Chem
PubMed ID12639953
'Multivesicular bodies (MVBs) are endocytic structures that contain small vesicles formed by the budding of an endosomal membrane into the lumen of the compartment. Fusion of MVBs with the plasma membrane results in secretion of the small internal vesicles termed exosomes. K562 cells are a hematopoietic cell line that releases ... More
Cellular activation by Ca2+ release from stores in the endoplasmic reticulum but not by increased free Ca2+ in the cytosol.
AuthorsStrayer DS, Hoek JB, Thomas AP, White MK
JournalBiochem J
PubMed ID10548531
'Ca(2+) release from intracellular stores and/or transmembrane influx can increase the cytosolic free Ca(2+) concentration ([Ca(2+)](i)). Such changes in [Ca(2+)](i) might transduce signals regulating transcription, motility, secretion, and so on. Surfactant secretagogues such as ATP and ionomycin stimulate the release and transmembrane influx of Ca(2+), both of which increase [Ca(2+)](i). ... More
Reactive oxygen species activate p90 ribosomal S6 kinase via Fyn and Ras.
AuthorsAbe J, Okuda M, Huang Q, Yoshizumi M, Berk BC
JournalJ Biol Chem
PubMed ID10636870
'Reactive oxygen species and growth factors stimulate similar intracellular signal transduction events including activation of Src kinase family members and extracellular signal-regulated kinases (ERK1/2). A potentially important downstream effector of Src and ERK1/2 is p90 ribosomal S6 kinase (p90RSK), which plays an important role in cell growth by activating several ... More
Novel modulatory effect of L-type calcium channels at newly formed neuromuscular junctions.
AuthorsSugiura Y, Ko CP
JournalJ Neurosci
PubMed ID8994064
'This study aimed to examine changes of presynaptic voltage-sensitive calcium channel (VSCC) subtypes during synapse formation and regeneration in relation to transmitter release at the neuromuscular junction (NMJ). Synaptic potentials were recorded from developing rat NMJs and from regenerating mouse and frog NMJs. As in normal adult NMJs, evoked transmitter ... More
Stimulation of adenylyl cyclase mediated by phospholipase C-linked M3 muscarinic receptor in human neuroblastoma SK-N-BE (2) C cells.
AuthorsSuh BC, Kim KT
JournalJ Neurochem
PubMed ID7760029
'Muscarinic receptor in human neuroblastoma SK-N-BE(2)C cells was identified and characterized. Treatment of the cells with carbachol evoked the generation of inositol 1,4,5-trisphosphate (IP3) with a peak level reached at 1 min after stimulation. Carbachol increased intracellular Ca2+ ([Ca2+]i) with an EC50 value of 35 microM. In addition, carbachol produced ... More
Convergence of CD19 and B cell antigen receptor signals at MEK1 in the ERK2 activation cascade.
AuthorsLi X, Carter RH
JournalJ Immunol
PubMed ID9834070
'CD19 plays a critical role in regulating B cell responses to Ag. We have studied the mechanism by which coligation of CD19 and the B cell Ag receptor, membrane Ig (mIg), augments signal transduction, including synergistic enhancement of release of intracellular Ca2+ and extracellular signal-regulated protein kinase 2 (ERK2) activation, ... More
The FcgammaRII receptor triggers pp125FAK phosphorylation in platelets.
AuthorsHaimovich B, Regan C, DiFazio L, Ginalis E, Ji P, Purohit U, Rowley RB, Bolen J, Greco R
JournalJ Biol Chem
PubMed ID8663117
'Platelets express a single low affinity receptor for immunoglobulin, FcgammaRII, that triggers multiple cellular responses upon interaction with multivalent immune complexes. In this study we show that immobilized IgG is also a potent stimulant of platelet activation triggering adhesion, aggregation, massive dense granule secretion, and thromboxane production. Platelet adhesion to ... More
Phorbol ester-induced changes in cytoplasmic Ca2+ in human neutrophils. Involvement of a pertussis toxin-sensitive G protein.
AuthorsNasmith PE, Grinstein S
JournalJ Biol Chem
PubMed ID3115980
'Activation of neutrophils by most soluble stimuli is associated with a marked increase in intracellular free Ca2+ ([Ca2+]i). However, under physiological conditions (Na+-rich media), the potent activator 12-O-tetradecanoylphorbol-13-acetate (TPA) causes no change or a decrease in [Ca2+]i. We report here that the [Ca2+]i response to phorbol esters varies depending on ... More
Calcium-induced acrosomal exocytosis requires cAMP acting through a protein kinase A-independent, Epac-mediated pathway.
AuthorsBranham MT, Mayorga LS, Tomes CN
JournalJ Biol Chem
PubMed ID16407249
'Epac, a guanine nucleotide exchange factor for the small GTPase Rap, binds to and is activated by the second messenger cAMP. In sperm, there are a number of signaling pathways required to achieve egg-fertilizing ability that depend upon an intracellular rise of cAMP. Most of these processes were thought to ... More
Calcium-dependent threonine phosphorylation of nonmuscle myosin in stimulated RBL-2H3 mast cells.
AuthorsBuxton DB, Adelstein RS
JournalJ Biol Chem
PubMed ID10945986
'Stimulation of RBL-2H3 m1 mast cells through the IgE receptor with antigen, or through a G protein-coupled receptor with carbachol, leads to the rapid appearance of phosphothreonine in nonmuscle myosin heavy chain II-A (NMHC-IIA). We demonstrate that this results from phosphorylation of Thr-1940 by calcium/calmodulin-dependent protein kinase II (CaM kinase ... More
Dependence of the Ca2+-inhibitable adenylyl cyclase of C6-2B glioma cells on capacitative Ca2+ entry.
AuthorsFagan KA, Mons N, Cooper DM
JournalJ Biol Chem
PubMed ID9535924
'The ability of adenylyl cyclases to be regulated by physiological transitions in Ca2+ provides a key point for integration of cytosolic Ca2+ concentration ([Ca2+]i) and cAMP signaling. Ca2+-sensitive adenylyl cyclases, whether endogenously or heterologously expressed, require Ca2+ entry for their regulation, rather than Ca2+ release from intracellular stores (Chiono, M., ... More
The role of Ca(2+) in stimulated bioluminescence of the dinoflagellate Lingulodinium polyedrum.
Authorsvon Dassow P, Latz MI
JournalJ Exp Biol
PubMed ID12200401
'Many marine dinoflagellates emit bright discrete flashes of light nearly instantaneously in response to either laminar or turbulent flows as well as to direct mechanical stimulation. The flash involves a unique pH-dependent luciferase and a proton-mediated action potential across the vacuole membrane. The mechanotransduction process initiating this action potential is ... More
Calcium fluxes in rat thyroid FRTL-5 cells. Evidence for Ca2+ entry after stimulation with ATP.
AuthorsTörnquist K
JournalMol Cell Endocrinol
PubMed ID1936540
'The relationship between ATP-induced uptake of 45Ca2+ and the ATP-induced changes in [Ca2+]i was investigated in rat FRTL-5 thyroid cells. Addition of 1 microCi 45Ca2+/ml together with ATP induced a time- and dose-dependent increase in uptake of 45Ca2+, the uptake being still significantly above control after 30 min. Resting intracellular ... More
Sphingosine 1-phosphate and isoform-specific activation of phosphoinositide 3-kinase beta. Evidence for divergence and convergence of receptor-regulated endothelial nitric-oxide synthase signaling pathways.
AuthorsIgarashi J, Michel T
JournalJ Biol Chem
PubMed ID11470796
'Sphingosine 1-phosphate (S1P) is a platelet-derived sphingolipid that elicits diverse biological responses, including angiogenesis, via the activation of G protein-coupled EDG receptors. S1P activates the endothelial isoform of nitric-oxide synthase (eNOS), associated with eNOS phosphorylation at Ser-1179, a site phosphorylated by protein kinase Akt. We explored the proximal signaling pathways ... More
Pyrimidinoceptor-mediated potentiation of inducible nitric-oxide synthase induction in J774 macrophages. Role of intracellular calcium.
AuthorsChen BC, Chou CF, Lin WW
JournalJ Biol Chem
PubMed ID9792689
'We have shown that, in murine J774 macrophages, binding of UTP to pyrimidinoceptors stimulates phosphoinositide (PI) breakdown and an increase in [Ca2+]i. In this study, UTP modulation of the expression of inducible nitric-oxide synthase (iNOS) was investigated. Although UTP alone had no effect, stimulation of J774 cells with a combination ... More
Changes in Trypanosoma cruzi infectivity by treatments that affect calcium ion levels.
AuthorsYakubu MA, Majumder S, Kierszenbaum F
JournalMol Biochem Parasitol
PubMed ID7984174
'The possible role of the intracellular Ca2+ level in the regulation of Trypanosoma cruzi infectivity was explored by measuring the capacity of trypomastigote forms of this organism to invade mammalian host cells after treatments which decrease or elevate cytoplasmic Ca2+. Parasites loaded with either bis-(o-aminophenoxy)-ethane-N,N,N'',N'' tetraacetic acid (BAPTA) or 2-([2-bis(carboxymethyl)-amino-5-methylphenoxy]methyl)-6-nethoxy-8 ... More
Cytosolic calmodulin is increased in SK-N-SH human neuroblastoma cells due to release of calcium from intracellular stores.
AuthorsMcGinnis KM, Shariat-Madar Z, Gnegy ME
JournalJ Neurochem
PubMed ID9422356
'Muscarinic receptor stimulation elicits a redistribution of calmodulin (CaM) from the membrane fraction to cytosol in the human neuroblastoma cell line SK-N-SH. Increasing the intracellular Ca2+ concentration with ionomycin also elevates cytosolic CaM. The aim of this study was to investigate the roles of extracellular and intracellular Ca2+ pools in ... More
Vectorial Ca2+ flux from the extracellular space to the endoplasmic reticulum via a restricted cytoplasmic compartment regulates inositol 1,4,5-trisphosphate-stimulated Ca2+ release from internal stores in vascular endothelial cells.
AuthorsCabello OA, Schilling WP
JournalBiochem J
PubMed ID8240234
'Depletion of the Ins(1,4,5)P3-sensitive intracellular Ca2+ store of vascular endothelial cells after selective inhibition of the endoplasmic-reticulum (ER) Ca2+ pump by thapsigargin or 2,5-di-t-butylhydroquinone (BHQ) increases Ca2+ influx from the extracellular space in the absence of phosphoinositide hydrolysis. One model to account for these results suggests a close association between ... More
Ligand-induced internalization and increased cell calcium are mediated via distinct structural elements in the carboxyl terminus of the epidermal growth factor receptor.
AuthorsChang CP, Kao JP, Lazar CS, Walsh BJ, Wells A, Wiley HS, Gill GN, Rosenfeld MG
JournalJ Biol Chem
PubMed ID1744139
'Signals that can mediate ligand-induced receptor internalization and calcium regulation are present in a 48-amino acid "calcium-internalization" domain in the C'' terminus of the epidermal growth factor (EGF) receptor. The basis of calcium and internalization regulation signalled by this 48-amino acid sequence was analyzed using deletion and substitution mutant receptors. ... More
Imaging extracellular waves of glutamate during calcium signaling in cultured astrocytes.
AuthorsInnocenti B, Parpura V, Haydon PG
JournalJ Neurosci
PubMed ID10684881
'A growing body of evidence proposes that glial cells have the potential to play a role as modulators of neuronal activity and synaptic transmission by releasing the neurotransmitter glutamate (Arague et al., 1999). We explore the spatial nature of glutamate release from astrocytes with an enzyme-linked assay system and CCD ... More
Thapsigargin potentiates histamine-stimulated HCl secretion in gastric parietal cells but does not mimic cholinergic responses.
AuthorsChew CS, Petropoulos AC
JournalCell Regul
PubMed ID1848793
'The role of calcium in control of HCl secretion by the gastric parietal cell was examined using a recently available intracellular calcium-releasing agent, thapsigargin, which has been shown, in some cell types, to induce sustained elevation of intracellular calcium ([Ca2+]i), an action that appears to be independent of inositol lipid ... More
Two components of glutamate exocytosis differentially affected by presynaptic modulation.
AuthorsHerrero I, Castro E, Miras-Portugal MT, Sánchez-Prieto J
JournalJ Neurochem
PubMed ID8931466
'The total Ca(2+)-dependent release of glutamate induced by depolarization of cerebrocortical nerve terminals with KCl was analyzed into a fast and a slow component. The fast component exhibited a decay time of < 1 s and accounted for 0.95 +/- 0.10 nmol of glutamate, whereas the slow component, which exhibited ... More
Brain-derived neurotrophic factor induces long-lasting Ca2+-activated K+ currents in rat visual cortex neurons.
AuthorsMizoguchi Y, Monji A, Nabekura J
JournalEur J Neurosci
PubMed ID12405954
'Brain-derived neurotrophic factor (BDNF) increases postsynaptic intracellular Ca2+ and modulates synaptic transmission in various types of neurons. Ca2+-activated K+ currents, opened mainly by intracellular Ca2+ elevation, contribute to hyperpolarization following action potentials and modulate synaptic transmission. We asked whether BDNF induces Ca2+-activated K+ currents by postsynaptic elevation of intracellular Ca2+ ... More
Intracellular calcium waves accompany neutrophil polarization, formylmethionylleucylphenylalanine stimulation, and phagocytosis: a high speed microscopy study.
AuthorsKindzelskii AL, Petty HR
JournalJ Immunol
PubMed ID12496384
'Using high sensitivity fluorescence imaging with shutter speeds approximately 600,000 times faster than those of video frames, we have characterized Ca2+ waves within cells in exquisite detail to reveal Ca2+ signaling routes. Polarized neutrophils exhibited a counterclockwise rotating ryanodine-sensitive juxtamembrane Ca2+ wave during temporal calcium spikes. During stimulation with fMLP, ... More
Involvement of Ca2+ in the formation of high molecular weight DNA fragments in thymocyte apoptosis.
AuthorsZhivotovsky B, Cedervall B, Jiang S, Nicotera P, Orrenius S
JournalBiochem Biophys Res Commun
PubMed ID8037702
'Internucleosomal DNA fragmentation (DNA laddering) and formation of apoptotic bodies have long been considered characteristic features of apoptosis. However, recent work has shown that formation of high molecular weight DNA fragments precedes internucleosomal cleavage and may involve mechanisms that differ from those responsible for DNA laddering. Here, we show that ... More
Facile platelet adhesion to collagen requires metabolic energy and actin polymerization and evokes intracellular free calcium mobilization.
AuthorsSmith JB, Dangelmaier C, Selak MA, Daniel JL
JournalJ Cell Biochem
PubMed ID1939367
'The attachment of platelets to collagen-coated microtiter plates at 20 degrees C was inhibited strongly by depletion of metabolic energy or by addition of cytochalasins and was slightly inhibited by the intracellular Ca2+ chelator BAPTA. In keeping with their respective potencies as inhibitors of actin polymerization, cytochalasins D and H ... More
Ca2+ signaling by distinct endothelin peptides in glomerular mesangial cells.
AuthorsSimonson MS, Dunn MJ
JournalExp Cell Res
PubMed ID1845794
'Ca2+ signaling by peptides of the endothelin (ET) gene family was studied in cultured glomerular mesangial cells. In addition to the increase in cytosolic free [Ca2+] ([Ca2+]i) previously described for ET-1, we also observed that ET-2, ET-3, and sarafotoxin S6b generate similar [Ca2+]i waveforms but with dissimilar potencies and kinetics. ... More
Ca2+ and Zn2+ dependence of DNA synthesis in untransformed and in Ha-ras(val-12)-expressing NIH 3T3 cells.
AuthorsBack CJ, Sistonen L, Enkvist MO, Heikkilä JE, Akerman KE
JournalExp Cell Res
PubMed ID8359224
'The role of Ca2+ and Zn2+ in the initiation of DNA synthesis in NIH 3T3 fibroblasts and c-Ha-ras(val-12) oncoprotein-expressing (NIH 3T3) cells has been studied. Entrapment of the Ca2+ chelator, BAPTA (30 microM), into the cells totally blocked a serum-induced rise in cytosolic free Ca2+ ([Ca2+]i) as determined with fura-2. ... More
Calcineurin promotes protein kinase C and c-Jun NH2-terminal kinase activation in the heart. Cross-talk between cardiac hypertrophic signaling pathways.
AuthorsDe Windt LJ, Lim HW, Haq S, Force T, Molkentin JD
JournalJ Biol Chem
PubMed ID10788473
'Multiple intracellular signaling pathways have been shown to regulate the hypertrophic growth of cardiomyocytes. Both necessary and sufficient roles have been described for the mitogen activated protein kinase(1) (MAPK) signaling pathway, specific protein kinase C (PKC) isoforms, and calcineurin. Here we investigate the interdependence between calcineurin, MAPK, and PKC isoforms ... More
Protein kinase C, a pivotal regulator of hamster egg activation, functions after elevation of intracellular free calcium.
'We investigated the signal transduction pathways that mediate activation of Syrian hamster eggs. Under conditions in which the concentration of intracellular free calcium ([Ca2+]i) is clamped low, activation of protein kinase C (PKC) can induce second polar body formation, reformation of the nuclear envelope, and decondensation of chromatin, as well ... More
Regulation of calcium influx across the plasma membrane of the human T-leukemic cell line, JURKAT: dependence on a rise in cytosolic free calcium can be dissociated from formation of inositol phosphates.
AuthorsNg J, Gustavsson J, Jondal M, Andersson T
JournalBiochim Biophys Acta
PubMed ID2163689
'A rise in the cytosolic free Ca2+ concentration due to both mobilization of Ca2+ from internal stores and influx of extracellular Ca2+ across the plasma membrane through ''second messenger-operated Ca2+ channels'' is one of the first transmembrane signals detected following activation of CD2 or CD3 receptors on T-cells. In this ... More
Ca2+-dependent production and release of IL-8 in human neutrophils.
AuthorsKuhns DB, Young HA, Gallin EK, Gallin JI
JournalJ Immunol
PubMed ID9780210
'IL-8, a potent neutrophil chemoattractant that is elevated about 200-fold in exudative neutrophils isolated from localized inflammatory sites in vivo, is thought to play a major role in recruitment of neutrophils to inflammatory sites. Incubation of peripheral blood neutrophils with thapsigargin, an inhibitor of the endoplasmic reticulum Ca2+-sequestering-ATPase, causes a ... More
Differential lymphocyte growth-modifying effects of oxidants: changes in cytosolic Ca+2.
AuthorsDuncan DD, Lawrence DA
JournalToxicol Appl Pharmacol
PubMed ID2506676
'An increase in the concentration of cytosolic Ca+2 ([Ca-2]i) is among the earliest changes seen in mitogen-stimulated lymphocytes and is a consequence of signal transduction which usually results in the initiation of cell cycle progression. However, increased [Ca+2]i has also been correlated with cytotoxicity. We have determined whether modulations of ... More
Phospholipase C-gamma 2 couples Bruton's tyrosine kinase to the NF-kappaB signaling pathway in B lymphocytes.
AuthorsPetro JB, Khan WN
JournalJ Biol Chem
PubMed ID11042193
'Mutations in the gene encoding Bruton''s tyrosine kinase (BTK) interfere with B cell proliferation and lead to an X-linked immunodeficiency in mice characterized by reduced B cell numbers. Recent studies have established that BTK transmits signals from the B cell antigen receptor (BCR) to transcription factor NF-kappaB, which in turn ... More
Evidence against a major role for Ca2+ in hypoxia-induced gene expression in human hepatoma cells (Hep3B).
AuthorsMetzen E, Fandrey J, Jelkmann W
JournalJ Physiol
PubMed ID10358107
'1. The human hepatoma cell line Hep3B is a widely used model for studies of hypoxia-related gene expression. Cytosolic free calcium concentration ([Ca2+]i) has been implicated in cellular oxygen-sensing processes. We investigated whether calcium ions have a significant impact on the production of erythropoietin (EPO) and vascular endothelial growth factor ... More
Prostaglandin F2 alpha-induced calcium transient in ovine large luteal cells: I. Alterations in cytosolic-free calcium levels and calcium flux.
'The effect of prostaglandin F2 alpha (PGF2 alpha) on cytosolic calcium homeostasis was studied in suspensions of ovine large or small luteal cells from superovulated ewes. In large cells loaded with fura-2 (AM), resting cytosolic-free calcium ([Ca2+]i) was 62 +/- 5 nM (Hanks'' medium, pH 7.15), and PGF2 alpha (0.5 ... More
Corticotropin-releasing factor increases protein kinase C activity by elevating membrane-bound alpha and beta isoforms.
AuthorsKiang JG, Wang X, McClain DE
JournalChin J Physiol
PubMed ID7705172
'Corticotropin-releasing factor (CRF) has been shown to attenuate vascular leakage in injured skin, mucous membrane, muscle, lung, and brain. We previously reported that CRF increases cytosolic free calcium concentrations ([Ca2+]i), cellular cAMP, and inositol trisphosphates in human epidermoid A-431 cells. This study identified protein kinase C isoforms in A-431 cells ... More
Calcium is necessary but not sufficient for the platelet-activating factor release in human neutrophils stimulated by physiological stimuli. Role of G-proteins.
AuthorsGomez-Cambronero J, Durstin M, Molski TF, Naccache PH, Sha'afi RI
JournalJ Biol Chem
PubMed ID2513317
'Platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycerol-3-phosphocholine; PAF) enhances the release of newly synthesized PAF as measured by [3H]acetate incorporation into PAF in human neutrophils. The response was dose-dependent, rapid, transient, and inhibitable by the PAF antagonist BN-52021. The non-metabolizable bioactive PAF analogue (C-PAF) but not lyso-PAF enhances the release of newly synthesized PAF. ... More
Apoptosis as a mechanism of tributyltin cytotoxicity to thymocytes: relationship of apoptotic markers to biochemical and cellular effects.
AuthorsRaffray M, McCarthy D, Snowden RT, Cohen GM
JournalToxicol Appl Pharmacol
PubMed ID8470116
'Recent in vitro studies have suggested that activation of apoptosis could account for the profound depletion of cortical thymocytes, which characterizes tributyltin (TBT) immunotoxicity. However, it has also been shown that TBT disrupts macromolecular synthesis and cellular energetics to an extent that might be expected to interfere with the initiation ... More
Active involvement of Ca2+ in mitotic progression of Swiss 3T3 fibroblasts.
AuthorsKao JP, Alderton JM, Tsien RY, Steinhardt RA
JournalJ Cell Biol
PubMed ID2114410
'Global Ca2+ transients have been observed to precede nuclear envelope breakdown and the onset of anaphase in Swiss 3T3 fibroblasts in 8% (vol/vol) FBS. The occurrence of these Ca2+ transients was dependent on intracellular stores. These Ca2+ transients could be (a) abolished by serum removal without halting mitosis, and (b) ... More
cAMP-dependent protein kinase induces cAMP-response element-binding protein phosphorylation via an intracellular calcium release/ERK-dependent pathway in striatal neurons.
AuthorsZanassi P, Paolillo M, Feliciello A, Avvedimento EV, Gallo V, Schinelli S
JournalJ Biol Chem
PubMed ID11139572
'Activation of the cAMP-dependent protein kinase A (PKA) pathway may induce cAMP-response element-binding protein (CREB) phosphorylation either directly or via cross-talk mechanisms with other signal transduction pathways. In this study, we have investigated in striatal primary cultures the mechanism by which activation of the cAMP/PKA-dependent pathway leads to CREB phosphorylation ... More
Functional colocalization of calcium and calcium-gated potassium channels in control of transmitter release.
'We examined, using physiological and morphological techniques, the distribution of Ca(2+)-gated K+ (gKca) channels relative to the location of Ca2+ channels and transmitter release sites at the frog neuromuscular junction (NM). Charybdotoxin (ChTx) and iberiotoxin, blockers of gKca channels with large conductances, increase transmitter release at the frog NMJ. Intracellular ... More
Mildly oxidized low density lipoprotein rapidly stimulates via activation of the lysophosphatidic acid receptor Src family and Syk tyrosine kinases and Ca2+ influx in human platelets.
AuthorsMaschberger P, Bauer M, Baumann-Siemons J, Zangl KJ, Negrescu EV, Reininger AJ, Siess W
JournalJ Biol Chem
PubMed ID10764819
'In contrast to native low density lipoprotein (LDL), mildly oxidized LDL (mox-LDL) induced platelet shape change and stimulated during shape change the tyrosine phosphorylation of specific proteins including Syk; the translocation of Src, Fyn, and Syk to the cytoskeleton; and the increase of cytosolic Ca(2+) due to mainly Ca(2+) entry. ... More
PKC-epsilon is required for mechano-sensitive activation of ERK1/2 in endothelial cells.
AuthorsTraub O, Monia BP, Dean NM, Berk BC
JournalJ Biol Chem
PubMed ID9395450
'Mechano-sensitive regulation of endothelial cells (EC) function by shear stress is critical for flow-induced vasodilation and gene expression. Previous studies by our laboratory demonstrated that shear stress activates the 44- and 42-kDa extracellular signal-regulated kinases (ERK1/2) in EC in a time- and force-dependent manner. ERK1/2 activation was inhibited by protein ... More
Depletion of intracellular calcium stores activates smooth muscle cell calcium-independent phospholipase A2. A novel mechanism underlying arachidonic acid mobilization.
AuthorsWolf MJ, Wang J, Turk J, Gross RW
JournalJ Biol Chem
PubMed ID8999823
'Herein we present multiple lines of evidence which demonstrate that depletion of internal calcium stores is both necessary and sufficient for the activation of calcium-independent phospholipase A2 during arginine vasopressin (AVP)-mediated mobilization of arachidonic acid in A-10 smooth muscle cells. First, AVP-induced [3H]arachidonic acid release was independent of increases in ... More
Protein kinase C regulates tyrosine phosphorylation of pp125FAK in platelets adherent to fibrinogen.
AuthorsHaimovich B, Kaneshiki N, Ji P
JournalBlood
PubMed ID8547637
'Platelet adhesion to immobilized fibrinogen stimulates the induction of tyrosine phosphorylation of multiple proteins. However, platelet spreading and tyrosine phosphorylation of three proteins, the focal adhesion kinase pp125FAK and proteins of 101 and 105 kD (pp101 and pp105), require a second adenosine diphosphate (ADP)-dependent costimulatory event. In this study we ... More