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引用和文献 (18)
Invitrogen™
BTC，AM，细胞可透过性
BTC, AM 是一种可渗透细胞的基于香豆素苯并噻唑的 Ca2+ 指示剂。它在结合 Ca2+ 后，较大激发波长从约 480 nm了解更多信息
| 货号 | 数量 |
|---|---|
| B6791 | 100 μg |
货号 B6791
价格（CNY)
4,420.00
Each
数量:
100 μg
价格（CNY)
4,420.00
Each
BTC, AM 是一种可渗透细胞的基于香豆素苯并噻唑的 Ca2+ 指示剂。它在结合 Ca2+ 后，较大激发波长从约 480 nm 变为 400 nm，允许比率式测量，基本不受染料染色细胞厚度不均匀、光漂白和染料泄漏的影响。BTC 对 Ca2+ 具有很高的选择性，Kd ∼7µM 允许对高细胞内 Ca2+ 水平进行定量。
了解有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的更多信息›
了解有关离子指示剂（包括钙、钾、pH 值和膜电位指示剂）的更多信息›
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
数量100 μg
运输条件室温
适用于（应用）细胞活力与增殖
适用于（设备）流式细胞仪
产品类型锌指示剂
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。
引用和文献 (18)
引用和文献
Abstract
AM-loading of fluorescent Ca2+ indicators into intact single fibers of frog muscle.
Journal:Biophys J
PubMed ID:9168048
'The AM loading of a number of different fluorescent Ca2+ indicators was compared in intact single fibers of frog muscle. Among the 13 indicators studied, loading rates (the average increase in the fiber concentration of indicator per first 60 min of loading) varied approximately 100-fold, from approximately 3 microM/h to
Significance of Na/Ca exchange for Ca2+ buffering and electrical activity in mouse pancreatic beta-cells.
Journal:Biophys J
PubMed ID:10096898
'We have combined the patch-clamp technique with microfluorimetry of the cytoplasmic Ca2+ concentration ([Ca2+]i) to characterize Na/Ca exchange in mouse beta-cells and to determine its importance for [Ca2+]i buffering and shaping of glucose-induced electrical activity. The exchanger contributes to Ca2+ removal at [Ca2+]i above 1 microM, where it accounts for
Photolysis-induced suppression of inhibition in rat hippocampal CA1 pyramidal neurons.
Journal:J Physiol
PubMed ID:11410632
'1. Whole cell patch clamp recording, Ca(2+) measurement with ratiometric fluorescent dyes and photolysis of caged Ca(2+) were combined to investigate the depolarization- and photolysis-induced suppression of inhibition (DSI and PSI) in rat hippocampal CA1 pyramidal cells. 2. A 5-s depolarization from -70 mV to 0 mV or a 6-s
Ionized intracellular calcium concentration predicts excitotoxic neuronal death: observations with low-affinity fluorescent calcium indicators.
Journal:J Neurosci
PubMed ID:9254679
'Cytosolic calcium ([Ca2+]i) is an important mediator of neuronal signal transduction, participating in diverse biochemical reactions that elicit changes in synaptic efficacy, metabolic rate, and gene transcription. Excessive [Ca2+]i also has been implicated as a cause of acute neuronal injury, although measurement of [Ca2+]i in living neurons by fluorescent calcium
Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC.
Journal:Cell Calcium
PubMed ID:10756974
'Accurate measurement of elevated intracellular calcium levels requires indicators with low calcium affinity and high selectivity. We examined fluorescence spectral properties and ionic specificity of three low-affinity, ratiometric indicators structurally related to Fura-2: mag-Fura-2 (furaptra), Fura-2FF, and BTC. The indicators differed in respect to their excitation wavelengths, affinity for Ca2+