Ion selectivities of the Ca(2+) sensors for exocytosis in rat phaeochromocytoma cells.
AuthorsKishimoto T, Liu TT, Ninomiya Y, Takagi H, Yoshioka T, Ellis-Davies GC, Miyashita Y, Kasai H
JournalJ Physiol
PubMed ID11410622
'1. The ion selectivities of the Ca(2+) sensors for the two components of exocytosis in rat phaeochromocytoma (PC12) cells were examined by measurement of membrane capacitance and amperometry. The cytosolic concentrations of metal ions were increased by photolysis of caged-Ca(2+) compounds and measured with low-affinity indicators benzothiazole coumarin (BTC) or ... More
Detection of heavy metals in water by fluorescence spectroscopy: on the way to a suitable sensor system.
AuthorsPrestel H, Gahr A, Niessner R
JournalFresenius J Anal Chem
PubMed ID11220577
In order to develop a fiber optical heavy metal ion detection system, the applicability of selected complexing agents with fluorescent properties has been studied. Beginning with the application of known chelators, like BTC-5N, Newport Green, neocuproine, and chromotropic acid, a sensor configuration has been found, which allows the detection of ... More
The actions of synaptically released zinc at hippocampal mossy fiber synapses.
AuthorsVogt K, Mellor J, Tong G, Nicoll R
JournalNeuron
PubMed ID10798403
Zn2+ is present at high concentrations in the synaptic vesicles of hippocampal mossy fibers. We have used Zn2+ chelators and the mocha mutant mouse to address the physiological role of Zn2+ in this pathway. Zn2+ is not involved in the unique presynaptic plasticities observed at mossy fiber synapses but is ... More