A new method to detect cadmium uptake in protoplasts.
AuthorsLindberg S, Landberg T, Greger M
JournalPlanta
PubMed ID15069560
The mechanism for cadmium (Cd2+) uptake into the cytosol of protoplasts from 5- to 7-day-old wheat seedlings (Triticum aestivum L. cv. Kadett) was investigated by a new method, using fluorescence microscopy and the heavy metal-specific fluorescent dye, 5-nitrobenzothiazole coumarin, BTC-5N. Cadmium fluorescence gradually increased in the cytosol of shoot and ... More
Extracellular zinc ions induces mitogen-activated protein kinase activity and protein tyrosine phosphorylation in bombesin-sensitive Swiss 3T3 fibroblasts.
AuthorsHansson A
JournalArch Biochem Biophys
PubMed ID8644999
The growth factor-like effect of zinc in vitro and in vivo, which has long been recognized was investigated with respect to its mechanisms of action. Addition of zinc chloride to bombesin-sensitive Swiss 3T3 mouse fibroblasts induced a fourfold stimulation in the cytosolic myelin basic protein kinase activity. The response was ... More
Effects of Hg2+ on cytosolic Ca2+ in isolated skate hepatocytes.
AuthorsNathanson MH, Mariwalla K, Ballatori N, Boyer JL
JournalCell Calcium
PubMed ID8581971
Hg2+ is an environmental pollutant that adversely affects a range of cellular functions, including those that regulate free cytosolic Ca2+ (Ca(i)2+). To investigate the mechanism of Hg(2+)-induced Ca(i)2+ signaling, we examined the effects of Hg2+ on Ca(i)2+ in isolated skate hepatocytes, and developed a method to assess cytosolic Hg2+ (Hgi2+) ... More
Role of intracellular Cd2+ in catecholamine release and lethality in PC12 cells.
AuthorsKanthasamy AG, Isom GE, Borowitz JL
JournalToxicol Lett
PubMed ID8553369
To evaluate the role of intracellular Cd2+ in catecholamine release and lethality in rat pheochromocytoma (PC12) cells the following results were obtained: [1] the presence of Cd2+ intracellularly was demonstrated with the Cd(2+)-sensitive fluorescent dye BTC-5N, [2] Cd2+ entry through Ca(2+)-channels was either blocked with nifedipine or diltiazem or increased ... More
Cellular localization of calcium, heavy metals, and metallothionein in lobster (Homarus americanus) hepatopancreas.
AuthorsChavez-Crooker P, Pozo P, Castro H, Dice MS, Boutet I, Tanguy A, Moraga D, Ahearn GA
JournalComp Biochem Physiol C Toxicol Pharmacol
PubMed ID14659455
This investigation combines confocal microscopy with the cation-specific fluorescent dyes Fluo-3 and BTC-5N to localize calcium and heavy metals along the length of intact lobster (Homarus americanus) hepatopancreatic tubules and isolated cells. A metallothionein-specific antibody, developed in mollusks with cross-reactivity in crustaceans, showed the tissue-specific occurrence of this metal-binding protein ... More
Diverse effects of metal chelating agents on the neuronal cytotoxicity of zinc in the hippocampus.
AuthorsCuajungco MP, Lees GJ
JournalBrain Res
PubMed ID9666092
Abnormal metabolism of metal ions such as zinc may contribute to neuropathology. Complexing zinc could reduce this pathology. Thus, to examine the effectiveness of metal chelating agents in vivo, a model system was used. This involved determining the ability of chelating agents to prevent neuronal death caused by zinc chloride ... More