Comparison of blue nucleic acid dyes for flow cytometric enumeration of bacteria in aquatic systems.
AuthorsLebaron P, Parthuisot N, Catala P
JournalAppl Environ Microbiol
PubMed ID9572943
'Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4'',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial ... More
The beta-catenin--TCF-1 pathway ensures CD4(+)CD8(+) thymocyte survival.
AuthorsIoannidis V, Beermann F, Clevers H, Held W
JournalNat Immunol
PubMed ID11477404
The association of trans-acting T cell factors (TCFs) or lymphoid enhancer factor 1 (LEF-1) with their coactivator beta-catenin mediates transient transcriptional responses to extracellular Wnt signals. We show here that T cell maturation depends on the presence of the beta-catenin--binding domain in TCF-1. This domain is necessary to mediate the ... More
Fluorescence staining and flow cytometry for monitoring microbial cells.
AuthorsVeal DA, Deere D, Ferrari B, Piper J, Attfield PV
JournalJ Immunol Methods
PubMed ID10986415
Large numbers of microbiological samples are analysed annually using traditional culture-based techniques. These techniques take hours to days to yield a result, are tedious and are not suitable for non-culturable microorganisms. Further, culture-based techniques do not provide real-time information on the physiological status of the organism in situ which is ... More
Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.
AuthorsDavey HM, Kell DB
JournalMicrobiol Rev
PubMed ID8987359
The most fundamental questions such as whether a cell is alive, in the sense of being able to divide or to form a colony, may sometimes be very hard to answer, since even axenic microbial cultures are extremely heterogeneous. Analyses that seek to correlate such things as viability, which is ... More