Equilibrium and kinetic measurements of muscarinic receptor antagonism on living cells using bead injection spectroscopy.
AuthorsHodder PS, Beeson C, Ruzicka J
JournalAnal Chem
PubMed ID10939374
'Bead injection spectroscopy (BIS) techniques are introduced for automated measurement of pharmacological antagonism by functional assay. Chinese hamster ovary cells that express the rat type 1 muscarinic receptor are cultured on microbeads and used as a renewable biological target for muscarinic receptor antagonist ligands. A flow injection instrument is used ... More
Activity-dependent expression and distribution of M1 muscarinic ACh receptors in visual cortex neuronal cultures.
AuthorsWang Y, Gu Q, Mao F, Haugland RP, Cynader MS
JournalJ Neurosci
PubMed ID8027768
The fluorescently labeled muscarinic M1 receptor-selective antagonist BODIPY FL pirenzepine has been employed to study the activity-dependent distribution and expression of muscarinic M1 ACh receptors (M1AChRs) in cultured neurons derived from rat visual cortex. Displacement experiments showed that like pirenzepine, binding of BODIPY FL pirenzepine was specific to M1 receptors ... More
Visualization of muscarinic cholinergic receptors on chick cardiomyocytes and their involvement in phosphatidylcholine hydrolysis.
AuthorsGoutsouliak V, Wang Y, Cynader MS, Rabkin SW
JournalBiochem Cell Biol
PubMed ID9250360
The purpose of this study was to visualize muscarinic receptors and their distribution on cardiomyocytes and to examine the effects of muscarinic cholinergic receptor (mACh-R) stimulation with carbachol on phosphatidylcholine hydrolysis. Cardiomyocytes were prepared as primary culture from 7-day-old chick embryo hearts. Cardiomyocytes, grown on cover slips, were labelled with ... More