The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors.
AuthorsReck-Peterson SL, Tyska MJ, Novick PJ, Mooseker MS
JournalJ Cell Biol
PubMed ID11381095
'The motor properties of the two yeast class V myosins, Myo2p and Myo4p, were examined using in vitro motility assays. Both myosins are active motors with maximum velocities of 4.5 microm/s for Myo2p and 1.1 microm/s for Myo4p. Myo2p motility is Ca(2+) insensitive. Both myosins have properties of a nonprocessive ... More
Cooperation between thrombospondin-1 type 1 repeat peptides and alpha(v)beta(3) integrin ligands to promote melanoma cell spreading and focal adhesion kinase phosphorylation.
AuthorsSipes JM, Krutzsch HC, Lawler J, Roberts DD
JournalJ Biol Chem
PubMed ID10428859
CD47-binding sequences from the carboxyl-terminal domain of thrombospondin-1 (TSP1) are known to regulate activity of the alpha(v)beta(3) integrin (Gao, G., Lindberg, F. P., Dimitry, J. M., Brown, E. J., and Frazier, W. A. (1996) J. Cell Biol. 135, 533-544). Here we show that peptides from the type 1 repeats of ... More
Identification of an alpha(3)beta(1) integrin recognition sequence in thrombospondin-1.
AuthorsKrutzsch HC, Choe BJ, Sipes JM, Guo N, Roberts DD
JournalJ Biol Chem
PubMed ID10446179
A synthetic peptide containing amino acid residues 190-201 of thrombospondin-1 (TSP1) promoted adhesion of MDA-MB-435 breast carcinoma cells when immobilized and inhibited adhesion of the same cells to TSP1 when added in solution. Adhesion to this peptide was enhanced by a beta(1) integrin-activating antibody, Mn(2+), and insulin-like growth factor I ... More