Exercise training attenuates coronary smooth muscle phenotypic modulation and nuclear Ca2+ signaling.
AuthorsWamhoff BR, Bowles DK, Dietz NJ, Hu Q, Sturek M
JournalAm J Physiol Heart Circ Physiol
PubMed ID12388302
'Physical inactivity is an independent risk factor for coronary heart disease, yet the mechanism(s) of exercise-related cardioprotection remains unknown. We tested the hypothesis that coronary smooth muscle after exercise training would have decreased mitogen-induced phenotypic modulation and enhanced regulation of nuclear Ca(2+). Yucatan swine were endurance exercise trained (EX) on ... More
Reaction diffusion modeling of calcium dynamics with realistic ER geometry.
AuthorsMeans S, Smith AJ, Shepherd J, Shadid J, Fowler J, Wojcikiewicz RJ, Mazel T, Smith GD, Wilson BS
JournalBiophys J
PubMed ID16617072
'We describe a finite-element model of mast cell calcium dynamics that incorporates the endoplasmic reticulum''s complex geometry. The model is built upon a three-dimensional reconstruction of the endoplasmic reticulum (ER) from an electron tomographic tilt series. Tetrahedral meshes provide volumetric representations of the ER lumen, ER membrane, cytoplasm, and plasma ... More
Nucleoplasmic Ca(2+)loading is regulated by mobilization of perinuclear Ca(2+).
AuthorsAbrenica B, Gilchrist JS
JournalCell Calcium
PubMed ID10970769
'Regulation of nucleoplasmic calcium (Ca(2+)) concentration may occur by the mobilization of perinuclear luminal Ca(2+)pools involving specific Ca(2+)pumps and channels of both inner and outer perinuclear membranes. To determine the role of perinuclear luminal Ca(2+), we examined freshly cultured 10 day-old embryonic chick ventricular cardiomyocytes. We obtained evidence suggesting the ... More
Depletion of calcium stores in injured sensory neurons: anatomic and functional correlates.
AuthorsGemes G, Rigaud M, Weyker PD, Abram SE, Weihrauch D, Poroli M, Zoga V, Hogan QH,
JournalAnesthesiology
PubMed ID19602957
'BACKGROUND: Painful nerve injury leads to disrupted Ca signaling in primary sensory neurons, including decreased endoplasmic reticulum (ER) Ca storage. This study examines potential causes and functional consequences of Ca store limitation after injury. METHODS: Neurons were dissociated from axotomized fifth lumbar (L5) and the adjacent L4 dorsal root ganglia ... More
Origin sites of calcium release and calcium oscillations in frog sympathetic neurons.
AuthorsMcDonough SI, Cseresnyés Z, Schneider MF
JournalJ Neurosci
PubMed ID11124983
'In many neurons, Ca(2+) signaling depends on efflux of Ca(2+) from intracellular stores into the cytoplasm via caffeine-sensitive ryanodine receptors (RyRs) of the endoplasmic reticulum. We have used high-speed confocal microscopy to image depolarization- and caffeine-evoked increases in cytoplasmic Ca(2+) levels in individual cultured frog sympathetic neurons. Although caffeine-evoked Ca(2+) ... More
Relocalization of STIM1 for activation of store-operated Ca(2+) entry is determined by the depletion of subplasma membrane endoplasmic reticulum Ca(2+) store.
AuthorsOng HL, Liu X, Tsaneva-Atanasova K, Singh BB, Bandyopadhyay BC, Swaim WD, Russell JT, Hegde RS, Sherman A, Ambudkar IS,
JournalJ Biol Chem
PubMed ID17298947
STIM1 (stromal interacting molecule 1), an endoplasmic reticulum (ER) protein that controls store-operated Ca(2+) entry (SOCE), redistributes into punctae at the cell periphery after store depletion. This redistribution is suggested to have a causal role in activation of SOCE. However, whether peripheral STIM1 punctae that are involved in regulation of ... More
New aspects of nuclear calcium signalling.
AuthorsGerasimenko O, Gerasimenko J
JournalJ Cell Sci
PubMed ID15226390
Nuclear calcium signalling has been a controversial battlefield for many years and the question of how permeable the nuclear pore complexes (NPCs) are to Ca2+ has been the subject of a particularly hot dispute. Recent data from isolated nuclei suggest that the NPCs are open even after depletion of the ... More
Homogeneous Ca2+ stores in rat adrenal chromaffin cells.
AuthorsInoue M, Sakamoto Y, Fujishiro N, Imanaga I, Ozaki S, Prestwich GD, Warashina A
JournalCell Calcium
PubMed ID12526884
The localization and function of Ca(2+) stores in isolated chromaffin cells of rat adrenal medulla were investigated using confocal laser microscopy and amperometry. Binding sites for BODIPY-inositol 1,4,5-trisphosphate (IP(3)), -ryanodine (Ry), and -thapsigargin (Thap) were both perinuclear and at the cell periphery. The endoplasmic reticulum (ER), which was identified by ... More
Ca2+ transport ATPase isoforms SERCA2a and SERCA2b are targeted to the same sites in the murine heart.
AuthorsVangheluwe P, Louch WE, Ver Heyen M, Sipido K, Raeymaekers L, Wuytack F
JournalCell Calcium
PubMed ID14572804
Adult SERCA2(b/b) mice expressing the non-muscle Ca2+ transport ATPase isoform SERCA2b in the heart instead of the normally predominant sarcomeric SERCA2a isoform, develop mild concentric ventricular hypertrophy with impaired cardiac contractility and relaxation [Circ. Res. 89 (2001) 838]. Results from a separate study on transgenic mice overexpressing SERCA2b in the ... More
Sorting of calcium signals at the junctions of endoplasmic reticulum and mitochondria.
AuthorsCsordás G, Hajnóczky G
JournalCell Calcium
PubMed ID11243933
Calcium signal transmission between endoplasmic reticulum (ER) and mitochondria is supported by a local [Ca(2+)] control that operates between IP(3)receptor Ca(2+)release channels (IP(3)R) and mitochondrial Ca(2+)uptake sites, and displays functional similarities to synaptic transmission. Activation of IP(3)R by IP(3)is known to evoke quantal Ca(2+)mobilization that is associated with incremental elevations ... More
Artemisinins target the SERCA of Plasmodium falciparum.
AuthorsEckstein-Ludwig U, Webb RJ, Van Goethem ID, East JM, Lee AG, Kimura M, O'Neill PM, Bray PG, Ward SA, Krishna S
JournalNature
PubMed ID12931192
Artemisinins are extracted from sweet wormwood (Artemisia annua) and are the most potent antimalarials available, rapidly killing all asexual stages of Plasmodium falciparum. Artemisinins are sesquiterpene lactones widely used to treat multidrug-resistant malaria, a disease that annually claims 1 million lives. Despite extensive clinical and laboratory experience their molecular target ... More
Functional visualization of the separate but interacting calcium stores sensitive to NAADP and cyclic ADP-ribose.
AuthorsLee HC, Aarhus R
JournalJ Cell Sci
PubMed ID11082034
Cells possess multiple Ca(2+) stores and their selective mobilization provides the spatial-temporal Ca(2+) signals crucial in regulating diverse cellular functions. Except for the inositol trisphosphate (IP(3))-sensitive Ca(2+) stores, the identities and the mechanisms of how these internal stores are mobilized are largely unknown. In this study, we describe two Ca(2+) ... More
C(a2+)-dependent glutamate release involves two classes of endoplasmic reticulum Ca(2+) stores in astrocytes.
AuthorsHua X, Malarkey EB, Sunjara V, Rosenwald SE, Li WH, Parpura V
JournalJ Neurosci Res
PubMed ID15048932
Astrocytes can modulate synaptic transmission by releasing glutamate in a Ca(2+)-dependent manner. Although the internal Ca(2+) stores have been implicated as the predominant source of Ca(2+) necessary for this glutamate release, the contribution of different classes of these stores is still not well defined. To address this issue, we cultured ... More
Role of sarcoplasmic/endoplasmic-reticulum Ca2+-ATPases in mediating Ca2+ waves and local Ca2+-release microdomains in cultured glia.
AuthorsSimpson PB, Russell JT
JournalBiochem J
PubMed ID9224652
We have characterized the sarcoplasmic-endoplasmic reticulum Ca2+-ATPase (SERCA) pumps in cultured rat cortical type-1 astrocytes, type-2 astrocytes and oligodendrocytes. Perfusion with 10 microM cyclopiazonic acid (CPA) or 1 microM thapsigargin evoked a large and persistent elevation in cytosolic [Ca2+] in normal Ca2+-containing medium and a small and transient increase in ... More
Intracellular calcium store depletion and acrosome reaction in human spermatozoa: role of calcium and plasma membrane potential.
AuthorsRossato M, Di Virgilio F, Rizzuto R, Galeazzi C, Foresta C
JournalMol Hum Reprod
PubMed ID11160837
We evaluated the presence and role of internal calcium stores in human uncapacitated spermatozoa by determining the effects of two inhibitors of Ca2+ ATPase of the sarco-endoplasmic reticulum (SERCA-ATPase), thapsigargin and cyclopiazonic acid (CPA) on intracellular calcium concentrations, [Ca2+](i), plasma membrane potential and acrosome reaction. Using a fluorescent conjugate of ... More
Functional expression of recombinant type 1 ryanodine receptor in insect cells.
AuthorsAntaramián A, Butanda-Ochoa A, Vázquez-Martínez O, Díaz-Muñoz M, Vaca L
JournalCell Calcium
PubMed ID11396983
We have investigated the biochemical properties of the rabbit ryanodine receptor type 1 (RyR1) from skeletal muscle functionally expressed in insect sf 21 cells infected with recombinant baculovirus. Equilibrium [3H]ryanodine binding assays applied to total membrane fractions from sf 21 cells expressing recombinant RyR1 showed a non-hyperbolic saturation curve (Hill ... More