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引用和文献 (25)
Invitrogen™
Bis-BODIPY™ FL C11-PC)（1,2-双-(4,4-二氟-5,7-二甲基-4-硼-3a,4a-二氮杂-s-茚烯-3-十一烷酰基)-sn-丙三醇-3-胆碱磷酸）
Molecular Probes™ 磷脂酶底物是使用染料标记的磷脂，用于监测纯化的酶制备物、细胞裂解物和活细胞中的磷脂酶 A (PLA) 活性。这些底物有两种结构变体，分别特异性用于 PLA1 和了解更多信息
| 货号 | 数量 |
|---|---|
| B7701 又称 B-7701 | 100 μg |
货号 B7701
又称 B-7701
价格（CNY)
4,804.00
Each
数量:
100 μg
价格（CNY)
4,804.00
Each
Molecular Probes™ 磷脂酶底物是使用染料标记的磷脂，用于监测纯化的酶制备物、细胞裂解物和活细胞中的磷脂酶 A (PLA) 活性。这些底物有两种结构变体，分别特异性用于 PLA1 和 PLA2。这些底物生成的切割产物的荧光标记剂型也可用作标准品，用于评估酶反应中底物转化的百分比。
荧光磷脂酶 A 底物规格：
• 标记（激发/发射波长）：Bis-BODIPY™ FL C11-PC (∼488/530 nm)
• 特异性：PLA1 或 PLA2
• 切割产物：D3862
• 冻干产品可溶于乙醇后使用
•荧光可通过微孔板检测或使用流式细胞分析检测
查找更多用于脂质代谢和脂肪酸类似物的探针
请参阅 Molecular Probes™ 手册中的脂肪酸类似物和磷脂—第 13.2 节以及用于脂质代谢和信号转导的探针—第 17.4 节，了解有关这些产品的更多信息。
供研究使用。不可用于人或动物的治疗或诊断。
荧光磷脂酶 A 底物规格：
• 标记（激发/发射波长）：Bis-BODIPY™ FL C11-PC (∼488/530 nm)
• 特异性：PLA1 或 PLA2
• 切割产物：D3862
• 冻干产品可溶于乙醇后使用
•荧光可通过微孔板检测或使用流式细胞分析检测
查找更多用于脂质代谢和脂肪酸类似物的探针
请参阅 Molecular Probes™ 手册中的脂肪酸类似物和磷脂—第 13.2 节以及用于脂质代谢和信号转导的探针—第 17.4 节，了解有关这些产品的更多信息。
供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
化学名称或材料Phospholipids
建议的储存条件在冷冻冰箱（-5 至 -30°C）中避光储存。
物理形态Solid
产品线BODIPY
数量100 μg
Unit SizeEach
引用和文献 (25)
引用和文献
Abstract
Characterization of Ca2+-dependent phospholipase A2 activity during zebrafish embryogenesis.
Journal:J Biol Chem
PubMed ID:10383445
'We have developed a simple fluorescent assay for detection of phospholipase A2 (PLA2) activity in zebrafish embryos that utilizes a fluorescent phosphatidylcholine substrate. By using this assay in conjunction with selective PLA2 inhibitors and Western blot analysis, we identified the principal activity in zebrafish embryogenesis as characteristic of the Ca2+-dependent
Down-regulation by elicitors of phosphatidylcholine-hydrolyzing phospholipase C and up-regulation of phospholipase A in plant cells.
Journal:Biochem Biophys Res Commun
PubMed ID:12054536
'Phosphatidylcholine, labeled by two fluorescent fatty acids, was fed to cultured plant cells (Petrosilenum crispum, L.; VBI-0, Nicotiana benthiana, L.) and fluorescent diacylglycerol (DAG) was the major metabolite. When a glycoprotein elicitor, derived from Phytophthora sojae, was applied to the parsley cells and the small protein cryptogein from Phytophthora cryptogea
Secretory phospholipase A2 is released from pancreatic beta-cells and stimulates insulin secretion via inhibition of ATP-dependent K+ channels.
Journal:Biochem Biophys Res Commun
PubMed ID:14521906
'The release of sPLA(2) from single mouse pancreatic beta-cells was monitored using a fluorescent substrate of the enzyme incorporated in the outer leaflet of the plasma membrane. Stimulation of beta-cells with agents that increased cytosolic free Ca(2+) concentration ([Ca(2+)](i)) induced a rapid release of sPLA(2) to the extracellular medium. Exogenous
Arachidonic acid causes cell death through the mitochondrial permeability transition. Implications for tumor necrosis factor-alpha aopototic signaling.
Journal:J Biol Chem
PubMed ID:11134037
'We have investigated the effects of arachidonic and palmitic acids in isolated rat liver mitochondria and in rat hepatoma MH1C1 cells. We show that both compounds induce the mitochondrial permeability transition (PT). At variance from palmitic acid, however, arachidonic acid causes a PT at concentrations that do not cause PT-independent
Activation by Cdc42 and PIP(2) of Wiskott-Aldrich syndrome protein (WASp) stimulates actin nucleation by Arp2/3 complex.
Journal:J Cell Biol
PubMed ID:10995437
'We purified native WASp (Wiskott-Aldrich Syndrome protein) from bovine thymus and studied its ability to stimulate actin nucleation by Arp2/3 complex. WASp alone is inactive in the presence or absence of 0.5 microM GTP-Cdc42. Phosphatidylinositol 4,5 bisphosphate (PIP(2)) micelles allowed WASp to activate actin nucleation by Arp2/3 complex, and this