Human IL-1 beta ELISA Kit - FAQs

View additional product information for Human IL-1 beta ELISA Kit - FAQs (BMS224-2TEN, BMS224-2)

2 product FAQs found

Do you offer the standards included in the IL-1 Human ELISA Kit as a standalone product?

Unfortunately, we do not offer the standards in this kit as a standalone product.

Do I need to use the standards in the IL-1 beta Human ELISA Kit (Cat No. BMS224-2, BMS224-2TEN) all at once, or can I store the remaining amount and use as needed? Do you offer the standards as a standalone product?

The IL-1 beta Human ELISA Kit comes with 2 vials of standards that do not need to be reconstituted at the same time. You can store the other vial of standard at -20 degrees C until needed. The other vial of standard can be reconstituted and then aliquoted to allow for multiple use. The volume to reconstitute is written on the vial itself (and changes lot to lot), but you would want to aliquot into micro-tubes with just enough volume plus with a little extra (to avoid volume loss) to begin preparing your standard curve.

For example, you add 225 µl of the reconstituted standard into an Eppendorf tube which gets serially diluted and is enough to prepare duplicates for the standard curve. Therefore all you need is 250 µl (225 µl+25 µl wiggle room/volume loss) of the reconstituted standard as an aliquot. Store the aliquots at minimum -20 degrees C. This will allow you to run the assay a minimum of 2x (2 vials of standard) or more if you aliquot as discussed above.

Keep in mind the kit only comes with 12 strips. You will use 2 strips each time you want to run the assay for the standards alone (as they should be run in duplicates), as you cannot reuse the standard curve/OD from one run of the assay for another set. Similarly, you would need 2 strips per set of samples/unknowns to run in duplicate.
So, at most this kit would allow 3 uses. (4 strips per use, 2 for standards, 2 for unknowns).
Further, should you wish to use these in 3 separate occasions, be sure to allow the foil bag to come to room temperature (to avoid condensation issues), remove the strips from the holder that you don’t need and place these back into the bag and immediately return the foil bag back to 4 degrees C with the desiccant in place.
We do recommend storing your samples at -20 degrees C until you have collected enough to run the assay as indicated.