NativePAGE™ Cathode Buffer Additive (20X) - FAQs

我们不推荐将NativePAGE样品缓冲液和电泳缓冲液与NuPAGE Bis-Tris凝胶一起使用。NuPAGE Invitrogen Bis-Tris凝胶经优化可用于变性条件，具有极低的工作pH（pH 7.0），大部分蛋白质难以在非变性条件下在该凝胶中迁移。

NativePAGE样品缓冲液和电泳缓冲液专为与NativePAGE Bis-Tris凝胶一起使用而设计。我们不推荐将这些缓冲液用于任何其他凝胶（包括NuPAGE Tris-Acetate凝胶或InvitrogenTris-甘氨酸凝胶）的非变性应用。对于那些凝胶，我们推荐使用Invitrogen Tris-甘氨酸系统的非变性样品缓冲液和电泳缓冲液。

NativePAGE样品缓冲液和电泳缓冲液专门用于NativePAGE Bis-Tris凝胶。我们不推荐将这些缓冲液与任何其他凝胶（包括NuPAGE Tris-甘氨酸凝胶）一起用于非变性应用。对于其他凝胶，我们推荐使用Invitrogen Tris-甘氨酸非变性样品和电泳缓冲液。

NativePAGE样品缓冲液和电泳缓冲液专门用于NativePAGE Bis-Tris凝胶。我们不推荐将这些缓冲液与任何其他凝胶（包括NuPAGE Tris-Acetate凝胶）一起用于非变性应用。对于其他凝胶，我们推荐使用Invitrogen Tris-甘氨酸非变性样品和电泳缓冲液。

The NativePAGE Invitrogen Bis-Tris Gel System is a pre-cast polyacrylamide mini gel system that provides a sensitive and high-resolution method for analyzing native membrane protein complexes, native soluble proteins, molecular mass estimations, and assessing purity of native proteins. It is based on the blue native polyacrylamide gel electrophoresis technique (BN PAGE) developed by Schagger and von Jagow.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

We recommend rinsing the membrane briefly in water, air drying and store it at room temperature in a ziplock bag. Do not place nitrocellulose in the freezer because it will shatter. Unlike PVDF, nitrocellulose membranes should never be pre-wetted in alcohol as it will cause them to shrivel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate gels. When running Tris-Acetate gels under native conditions, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.