NativePAGE™ 5% G-250 Sample Additive - FAQs

我们不推荐将NativePAGE样品缓冲液和电泳缓冲液与NuPAGE Bis-Tris凝胶一起使用。NuPAGE Invitrogen Bis-Tris凝胶经优化可用于变性条件，具有极低的工作pH（pH 7.0），大部分蛋白质难以在非变性条件下在该凝胶中迁移。

NativePAGE样品缓冲液和电泳缓冲液专为与NativePAGE Bis-Tris凝胶一起使用而设计。我们不推荐将这些缓冲液用于任何其他凝胶（包括NuPAGE Tris-Acetate凝胶或InvitrogenTris-甘氨酸凝胶）的非变性应用。对于那些凝胶，我们推荐使用Invitrogen Tris-甘氨酸系统的非变性样品缓冲液和电泳缓冲液。

NativePAGE 5% G-250样品添加剂是一种Coomassie G-250的浓缩型储液，专为与含有洗涤剂（非离子型）的NativePAGE凝胶电泳样品一起使用而设计。正常情况下，非变性蛋白在凝胶上的迁移取决于凝胶缓冲液pH下的自然电荷/等电点，但是，加入Coomassie G-250会使蛋白质产生一个关联后的负电荷，即使是通常具有正电荷的高pI蛋白质也能带上负电。该添加剂能够与蛋白质非特异性结合，无需变性。NativePAGE 5% G-250样品添加剂应加入到阴极缓冲液中，也可加到样品中。

NativePAGE 5% G-250样品添加剂（货号BN2004）是一种浓缩型Coomassie G-250储液，专为与含有洗涤剂（非离子型）的NativePAGE凝胶电泳样品一起使用而设计。NativePAGE 5% G-250样品添加剂的具体成分保密。该添加剂不含洗涤剂。

NativePAGE样品缓冲液和电泳缓冲液专门用于NativePAGE Bis-Tris凝胶。我们不推荐将这些缓冲液与任何其他凝胶（包括NuPAGE Tris-甘氨酸凝胶）一起用于非变性应用。对于其他凝胶，我们推荐使用Invitrogen Tris-甘氨酸非变性样品和电泳缓冲液。

NativePAGE样品缓冲液和电泳缓冲液专门用于NativePAGE Bis-Tris凝胶。我们不推荐将这些缓冲液与任何其他凝胶（包括NuPAGE Tris-Acetate凝胶）一起用于非变性应用。对于其他凝胶，我们推荐使用Invitrogen Tris-甘氨酸非变性样品和电泳缓冲液。

We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The NativePAGE 5% G-250 Sample Additive is a concentrated stock solution of Coomassie G-250 designed for use with detergent (non-ionic) containing samples prepared for NativePAGE gel electrophoresis. Native proteins normally migrate on the gel according to natural charge/pI in the pH of gel buffer, but adding the Coomassie G-250 renders an associated negative charge even to high pI proteins that would normally have a positive charge. It binds to proteins non-specifically without denaturing them. The NativePAGE 5% G-250 Sample Additive is added to the cathode buffer and, optionally, to samples as well.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The NativePAGE 5% G-250 Sample Additive (Cat. No. BN2004) is a concentrated stock solution of Coomassie G-250 designed for use with detergent (non-ionic) containing samples prepared for NativePAGE gel electrophoresis. The exact composition of the NativePAGE 5% G-250 Sample Additive is proprietary. It does not contain a detergent.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate gels. When running Tris-Acetate gels under native conditions, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.