Question 1

能否将NativePAGE样品缓冲液和电泳缓冲液与NuPAGE Bis-Tris凝胶一起使用？

Accepted Answer

我们不推荐将NativePAGE样品缓冲液和电泳缓冲液与NuPAGE Bis-Tris凝胶一起使用。NuPAGE Invitrogen Bis-Tris凝胶经优化可用于变性条件，具有极低的工作pH（pH 7.0），大部分蛋白质难以在非变性条件下在该凝胶中迁移。

Question 2

能否将NativePAGE样品缓冲液和电泳缓冲液用于Invitrogen Tris-甘氨酸凝胶或NuPAGE Tris-Acetate凝胶的非变性应用？

Accepted Answer

NativePAGE样品缓冲液和电泳缓冲液专为与NativePAGE Bis-Tris凝胶一起使用而设计。我们不推荐将这些缓冲液用于任何其他凝胶（包括NuPAGE Tris-Acetate凝胶或InvitrogenTris-甘氨酸凝胶）的非变性应用。对于那些凝胶，我们推荐使用Invitrogen Tris-甘氨酸系统的非变性样品缓冲液和电泳缓冲液。

Question 3

能否将NativePAGE样品和电泳缓冲液与NuPAGE Tris甘氨酸凝胶一起用于非变性应用？

Accepted Answer

NativePAGE样品缓冲液和电泳缓冲液专门用于NativePAGE Bis-Tris凝胶。我们不推荐将这些缓冲液与任何其他凝胶（包括NuPAGE Tris-甘氨酸凝胶）一起用于非变性应用。对于其他凝胶，我们推荐使用Invitrogen Tris-甘氨酸非变性样品和电泳缓冲液。

Question 4

能否将NativePAGE样品和电泳缓冲液与NuPAGE Tris-Acetate凝胶一起用于非变性应用？

Accepted Answer

NativePAGE样品缓冲液和电泳缓冲液专门用于NativePAGE Bis-Tris凝胶。我们不推荐将这些缓冲液与任何其他凝胶（包括NuPAGE Tris-Acetate凝胶）一起用于非变性应用。对于其他凝胶，我们推荐使用Invitrogen Tris-甘氨酸非变性样品和电泳缓冲液。

Question 5

I would like to run a NativePAGE gel. Which of your protein standards should I use?

Accepted Answer

We recommend using the NativeMark Unstained Protein Standard, Cat. No. LC0725 for native gel electrophoresis with Tris-Glycine, NuPAGE Tris-Acetate or NativePAGE gels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Question 6

Can I use the NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels?

Accepted Answer

We do not recommend using NativePAGE Sample and Running buffers with NuPAGE Bis-Tris gels. NuPAGE Invitrogen Bis-Tris gels are optimized for denaturing conditions and have an extremely low operating pH (pH 7.0), which makes it difficult for most proteins to migrate through them in a native state.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Question 7

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels or NuPAGE Tris-Acetate gels for native applications?

Accepted Answer

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate or Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Question 8

Can I use the NativePAGE Sample and Running buffers with Invitrogen Tris-Glycine gels for native applications?

Accepted Answer

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including Invitrogen Tris-Glycine gels. For those gels, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Question 9

Can I use the NativePAGE Sample and Running buffers with NuPAGE Tris-Acetate gels for native applications?

Accepted Answer

NativePAGE Sample Buffers and Running buffers were developed specifically for use with the NativePAGE Bis-Tris gels. We do not recommend using these buffers for native applications with any other gels, including NuPAGE Tris-Acetate gels. When running Tris-Acetate gels under native conditions, we recommend using the Invitrogen Tris-Glycine Native Sample and running buffers.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

NativePAGE™ Running Buffer Kit - FAQs