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查看更多产品信息 Human Epidermal Keratinocytes, neonatal (HEKn) - FAQs (C0015C)
20 个常见问题解答
这些是衰老细胞，它们在成体角质形成细胞培养物中很常见。通常情况下培养物中会有一群衰老而不再增殖的细胞。而年轻细胞的个体更小，仍能够继续分裂。当培养物逐渐衰老，您就会观察到越来越多的大细胞，培养物最终也将停止生长。在正确培养和维持的情况下，这些培养物至少能够完成25次的群体倍增生长。
这些是衰老细胞，在成体角质形成细胞培养物中很常见。通常情况下培养物中会有一群衰老而不再增殖的细胞。而年轻细胞的个体更小，仍能够继续分裂。当培养物逐渐衰老，您就会观察到越来越多的大体积细胞，培养物最终也将停止生长。在正确培养和维持的情况下，这些培养物至少能够完成25次的群体倍增。
每一批细胞均经过倍增时间测试，相应测试结果记录在COA中。
这些信息会显示在COA中。
它们是同种细胞。角质形成细胞也称为基底细胞或基底角质形成细胞。
对不同的原代细胞我们使用不同的培养基和添加剂。更多详细信息可查看此处 (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).
通过每一培养物的接种密度和收获密度来计算累计群体倍增水平。
这一参数随着供体年龄、组织大小、位置及我们的设备条件而发生着很大变化。某些情况下，少量组织即可分离很多细胞；而某些情况下，大量组织只能取得少量细胞。通常情况下，我们可获得30-300管细胞。
是的，我们从组织中分离原代细胞需要使用酶进行消化。
原代细胞是从活体组织（例如活检材料）中直接获取，并在体外培养的细胞。由于这些细胞经历极少的群体倍增，因此比连续（肿瘤或人工永生化的）细胞系更能代表其来源组织的主要功能成分，使原代细胞成为体内状态更具代表性的模型。
通过使用不同种属来源的原代细胞，您可了解人类与临床前测试物种之间可能存在的差异。在开展体内研究之前，可使用小鼠或大鼠细胞摸索用量并减少临床前毒理实验所需的动物数量。人细胞可用于确定从动物模型外推所得人类数据的准确性。
These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care an attention, the culture should yield at least 25 population doublings.
These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size, should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care and attention, the culture should yield at least 25 population doublings.
Each lot is tested for doubling time and the results are listed on the COA.
This information also appears on the COA.
These are all the same. Sometimes Keratinocytes are referred to as basal cells or basal keratinocytes.
We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).
Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.
It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.
Yes, our primary cells are isolated from tissue using enzymatic digestion.
Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for growth in vitro. These cells have undergone very few population doublings and are therefore more representative of the main functional component of the tissue from which they are derived in comparison to continuous (tumor or artificially immortalized) cell lines, making primary cells a more representative model for the in vivo state.
Primary cells from different species may be used, allowing you to highlight potential differences between humans and preclinical test species. Before in vivo studies, mouse or rat cells can be used to refine doses and reduce the number of animals required for preclinical toxicology. Human cells can be used to determine the accuracy of extrapolating human data from an animal model.