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查看更多产品信息 Human Dermal Fibroblasts, adult (HDFa) - FAQs (C0135C)
13 个常见问题解答
HDFn细胞生长于加入了低血清生长添加剂（Low Serum Growth Supplement）的106培养基中。
对不同的原代细胞我们使用不同的培养基和添加剂。更多详细信息可查看此处 (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).
通过每一培养物的接种密度和收获密度来计算累计群体倍增水平。
这一参数随着供体年龄、组织大小、位置及我们的设备条件而发生着很大变化。某些情况下，少量组织即可分离很多细胞；而某些情况下，大量组织只能取得少量细胞。通常情况下，我们可获得30-300管细胞。
是的，我们从组织中分离原代细胞需要使用酶进行消化。
原代细胞是从活体组织（例如活检材料）中直接获取，并在体外培养的细胞。由于这些细胞经历极少的群体倍增，因此比连续（肿瘤或人工永生化的）细胞系更能代表其来源组织的主要功能成分，使原代细胞成为体内状态更具代表性的模型。
通过使用不同种属来源的原代细胞，您可了解人类与临床前测试物种之间可能存在的差异。在开展体内研究之前，可使用小鼠或大鼠细胞摸索用量并减少临床前毒理实验所需的动物数量。人细胞可用于确定从动物模型外推所得人类数据的准确性。
Human Dermal Fibroblasts, adult (HDFa) (Cat. No. C0135C) and Human Dermal Fibroblasts, neonatal (HDFn) (Cat. No. C0045C) are primary human dermal fibroblasts isolated from adult skin and neonatal foreskin, respectively. They are cryopreserved at the end of the primary culture stage and not passaged.
HDF cells are grown in Medium 106 with the addition of Low Serum Growth Supplement.
We use various media and supplements for the various primary cells. Details can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/primary-cell-culture.html).
Cumulative population doublings are calculated from the seeding density and harvesting density from each culture level.
It varies a lot depending on the age of donor, size of tissues, locations, and conditions arrived to our facility. Sometime a small amount of tissue gives us many cells and sometimes a large amount of tissue gives us a very small lot. Typically, we can get 30-300 vials.
Yes, our primary cells are isolated from tissue using enzymatic digestion.
Primary cells are cells taken directly from living tissue (e.g., biopsy material) and established for growth in vitro. These cells have undergone very few population doublings and are therefore more representative of the main functional component of the tissue from which they are derived in comparison to continuous (tumor or artificially immortalized) cell lines, making primary cells a more representative model for the in vivo state.
Primary cells from different species may be used, allowing you to highlight potential differences between humans and preclinical test species. Before in vivo studies, mouse or rat cells can be used to refine doses and reduce the number of animals required for preclinical toxicology. Human cells can be used to determine the accuracy of extrapolating human data from an animal model.