HCEC-3 500K CELLS⁄VIAL - FAQs

查看更多产品信息 HCEC-3 500K CELLS⁄VIAL - FAQs (C0195C)

2 个常见问题解答

你们推荐使用何种培养基来培养人角膜上皮细胞（human corneal epithelial cell，HCEC）细胞？

我们推荐在无血清的角质形成细胞培养基（KSFM）中培养HCEC细胞，这些细胞也是使用这款培养基生产和质控的。不过，如果需要成份明确的生长系统，我们推荐您使用成份确定的KSFM培养基（Defined Keratinocyte-SFM）。请注意在成份确定的KSFM培养基中培养HCEC细胞时需要使用基质包被试剂盒（Coating Matrix Kit）。内部测试显示KSFM与成份确定的KSFM培养基中培养的HCEC细胞具有相似的生长速率和形态。

What media do you recommend for culturing Human Corneal Epithelial Cells (HCEC)?

We recommend culturing HCECs in keratinocyte serum-free medium (KSFM), as the cells are manufactured and quality-controlled using this medium. However, if a defined growth system is preferred, we recommend defined keratinocyte serum-free medium (Defined Keratinocyte-SFM). Note that a Coating Matrix Kit is required for culturing HCECs in Defined Keratinocyte-SFM. In-house testing has shown comparable HCEC growth and morphology in both KSFM and Defined Keratinocyte-SFM. Please note that these cells have been discontinued.