Amino acid residues critical for endoplasmic reticulum export and trafficking of platelet-activating factor receptor.
AuthorsHirota N, Yasuda D, Hashidate T, Yamamoto T, Yamaguchi S, Nagamune T, Nagase T, Shimizu T, Nakamura M,
JournalJ Biol Chem
PubMed ID20007715
'Several residues are conserved in the transmembrane domains (TMs) of G-protein coupled receptors. Here we demonstrate that a conserved proline, Pro(247), in TM6 of platelet-activating factor receptor (PAFR) is required for endoplasmic reticulum (ER) export and trafficking after agonist-induced internalization. Alanine-substituted mutants of the conserved residues of PAFRs, including P247A, ... More
Thiazolides, a new class of anti-influenza molecules targeting viral hemagglutinin at the post-translational level.
AuthorsRossignol JF, La Frazia S, Chiappa L, Ciucci A, Santoro MG,
JournalJ Biol Chem
PubMed ID19638339
'The emergence of highly contagious influenza A virus strains, such as the new H1N1 swine influenza, represents a serious threat to global human health. Efforts to control emerging influenza strains focus on surveillance and early diagnosis, as well as development of effective vaccines and novel antiviral drugs. Herein we document ... More
Common lymphatic endothelial and vascular endothelial receptor-1 mediates the transmigration of regulatory T cells across human hepatic sinusoidal endothelium.
'The common lymphatic endothelial and vascular endothelial receptor (CLEVER-1; also known as FEEL-1 and stabilin-1) is a recycling and intracellular trafficking receptor with multifunctional properties. In this study, we demonstrate increased endothelial expression of CLEVER-1/stabilin-1 at sites of leukocyte recruitment to the inflamed human liver including sinusoids, septal vessels, and ... More
The Giardia median body protein is a ventral disc protein that is critical for maintaining a domed disc conformation during attachment.
AuthorsWoessner DJ, Dawson SC,
JournalEukaryot Cell
PubMed ID22247266
'Giardia has unique microtubule structures, including the ventral disc, the primary organelle of attachment to the host, and the median body, a structure of undefined function. During attachment, the ventral disc has a domed conformation and enables Giardia to attach to the host intestinal epithelia within seconds. The mechanism of ... More
Streptolysin O clearance through sequestration into blebs that bud passively from the plasma membrane.
'Cells survive exposure to bacterial pore-forming toxins, such as streptolysin O (SLO), through mechanisms that remain unclear. Previous studies have suggested that these toxins are cleared by endocytosis. However, the experiments reported here failed to reveal any evidence for endocytosis of SLO, nor did they reveal any signs of damage ... More
Cellular complexity captured in durable silica biocomposites.
'Tissue-derived cultured cells exhibit a remarkable range of morphological features in vitro, depending on phenotypic expression and environmental interactions. Translation of these cellular architectures into inorganic materials would provide routes to generate hierarchical nanomaterials with stabilized structures and functions. Here, we describe the fabrication of cell/silica composites (CSCs) and their ... More
The non-phagocytic route of collagen uptake: a distinct degradation pathway.
AuthorsMadsen DH, Ingvarsen S, Jürgensen HJ, Melander MC, Kjøller L, Moyer A, Honoré C, Madsen CA, Garred P, Burgdorf S, Bugge TH, Behrendt N, Engelholm LH,
JournalJ Biol Chem
PubMed ID21652704
'The degradation of collagens, the most abundant proteins of the extracellular matrix, is involved in numerous physiological and pathological conditions including cancer invasion. An important turnover pathway involves cellular internalization and degradation of large, soluble collagen fragments, generated by initial cleavage of the insoluble collagen fibers. We have previously observed ... More
Membrane invaginations facilitate reversible water flux driving tunable iridescence in a dynamic biophotonic system.
AuthorsDeMartini DG, Krogstad DV, Morse DE,
JournalProc Natl Acad Sci U S A
PubMed ID23359694
'Squids have used their tunable iridescence for camouflage and communication for millions of years; materials scientists have more recently looked to them for inspiration to develop new ' ... More
Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells.
AuthorsLuo Q, Kumar P, Vickers TJ, Sheikh A, Lewis WG, Rasko DA, Sistrunk J, Fleckenstein JM,
Journal
PubMed ID24478067
'Enterotoxigenic Escherichia coli (ETEC) is a leading cause of death due to diarrheal illness among young children in developing countries, and there is currently no effective vaccine. Many elements of ETEC pathogenesis are still poorly defined. Here we demonstrate that YghJ, a secreted ETEC antigen identified in immunoproteomic studies using ... More
Two-photon imaging of the cornea visualized in the living mouse using vital dyes.
AuthorsZhang H, Wang L, Liu S, Xie Y, Deng X, He S, Zhang J, Sun S, Li X, Li Z,
Journal
PubMed ID24030459
'To acquire morphological and component information on the overall cornea in the living C57BL/6 mouse using fluorescent viability dyes and two-photon (2PH) laser microscopy. Corneas were scanned using a 2PH laser scanning fluorescence microscope after staining with plasma membrane stain and Hoechst 33342. Representative 2PH images of corneal cells were ... More
Estrogen induces rapid translocation of estrogen receptor beta, but not estrogen receptor alpha, to the neuronal plasma membrane.
'Estrogen receptors can activate transcription in the nucleus, and activate rapid signal transduction cascades in the cytosol. Multiple reports identify estrogen receptors at the plasma membrane, while others document the dynamic responses of estrogen receptor to ligand binding. However, the function and identity of membrane estrogen receptors remain controversial. We ... More
Imaging the Alphavirus Exit Pathway.
AuthorsMartinez MG, Snapp EL, Perumal GS, Macaluso FP, Kielian M,
Journal
PubMed ID24696489
'Alphaviruses are small enveloped RNA viruses with highly organized structures that exclude host cell proteins. They contain an internal nucleocapsid and an external lattice of the viral E2 and E1 transmembrane proteins. Alphaviruses bud from the plasma membrane (PM), but the process and dynamics of alphavirus assembly and budding are ... More
Lipin is a central regulator of adipose tissue development and function in Drosophila melanogaster.
AuthorsUgrankar R, Liu Y, Provaznik J, Schmitt S, Lehmann M,
JournalMol Cell Biol
PubMed ID21300783
'Lipins are evolutionarily conserved proteins found from yeasts to humans. Mammalian and yeast lipin proteins have been shown to control gene expression and to enzymatically convert phosphatidate to diacylglycerol, an essential precursor in triacylglcerol (TAG) and phospholipid synthesis. Loss of lipin 1 in the mouse, but not in humans, leads ... More
Cofilin-mediated F-actin severing is regulated by the Rap GTPase and controls the cytoskeletal dynamics that drive lymphocyte spreading and BCR microcluster formation.
AuthorsFreeman SA, Lei V, Dang-Lawson M, Mizuno K, Roskelley CD, Gold MR,
JournalJ Immunol
PubMed ID22068232
'When lymphocytes encounter APCs bearing cognate Ag, they spread across the surface of the APC to scan for additional Ags. This is followed by membrane contraction and the formation of Ag receptor microclusters that initiate the signaling reactions that lead to lymphocyte activation. Breakdown of the submembrane cytoskeleton is likely ... More
Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways.
AuthorsOlofsson A, Nygård Skalman L, Obi I, Lundmark R, Arnqvist A,
Journal
PubMed ID24846379
Bacteria shed a diverse set of outer membrane vesicles that function as transport vehicles to deliver effector molecules and virulence factors to host cells. Helicobacter pylori is a gastric pathogen that infects half of the world's population, and in some individuals the infection progresses into peptic ulcer disease or gastric ... More
Curcumin targets breast cancer stem-like cells with microtentacles that persist in mammospheres and promote reattachment.
Cancer stem-like cells (CSC) and circulating tumor cells (CTC) have related properties associated with distant metastasis, but the mechanisms through which CSCs promote metastasis are unclear. In this study, we report that breast cancer cell lines with more stem-like properties display higher levels of microtentacles (McTN), a type of tubulin-based ... More
Non-invasive neural stem cells become invasive in vitro by combined FGF2 and BMP4 signaling.
Neural stem cells (NSCs) typically show efficient self-renewal and selective differentiation. Their invasion potential, however, is not well studied. In this study, Sox2-positive NSCs from the E14.5 rat cortex were found to be non-invasive and showed only limited migration in vitro. By contrast, FGF2-expanded NSCs showed a strong migratory and ... More
Low pH, aluminum, and phosphorus coordinately regulate malate exudation through GmALMT1 to improve soybean adaptation to acid soils.
AuthorsLiang C, Piñeros MA, Tian J, Yao Z, Sun L, Liu J, Shaff J, Coluccio A, Kochian LV, Liao H,
JournalPlant Physiol
PubMed ID23341359
Low pH, aluminum (Al) toxicity, and low phosphorus (P) often coexist and are heterogeneously distributed in acid soils. To date, the underlying mechanisms of crop adaptation to these multiple factors on acid soils remain poorly understood. In this study, we found that P addition to acid soils could stimulate Al ... More
Cellular self-organization by autocatalytic alignment feedback.
AuthorsJunkin M, Leung SL, Whitman S, Gregorio CC, Wong PK,
JournalJ Cell Sci
PubMed ID22193956
Myoblasts aggregate, differentiate and fuse to form skeletal muscle during both embryogenesis and tissue regeneration. For proper muscle function, long-range self-organization of myoblasts is required to create organized muscle architecture globally aligned to neighboring tissue. However, how the cells process geometric information over distances considerably longer than individual cells to ... More
The gap junction protein Cx43 regulates B-lymphocyte spreading and adhesion.
AuthorsMachtaler S, Dang-Lawson M, Choi K, Jang C, Naus CC, Matsuuchi L,
JournalJ Cell Sci
PubMed ID21750189
The gap junction protein connexin43 (Cx43) is widely expressed in mammalian cells and forms intercellular channels for the transfer of small molecules between adjacent cells, as well as hemichannels that mediate bidirectional transport of molecules between the cell and the surrounding environment. Cx43 regulates cell adhesion and migration in neurons ... More
Human TMEM30a promotes uptake of antitumor and bioactive choline phospholipids into mammalian cells.
AuthorsChen R, Brady E, McIntyre TM,
JournalJ Immunol
PubMed ID21289302
Antitumor alkylphospholipids initiate apoptosis in transformed HL-60 and Jurkat cells while sparing their progenitors. 1-O-Alkyl-2-carboxymethyl-sn-glycero-3-phosphocholine (Edelfosine) like other short-chained phospholipids--inflammatory platelet-activating factor (PAF) and apoptotic oxidatively truncated phospholipids--are proposed to have intracellular sites of action, yet a conduit for these choline phospholipids into mammalian cells is undefined. Edelfosine is also ... More
Epithelial-to-mesenchymal transition promotes tubulin detyrosination and microtentacles that enhance endothelial engagement.
AuthorsWhipple RA, Matrone MA, Cho EH, Balzer EM, Vitolo MI, Yoon JR, Ioffe OB, Tuttle KC, Yang J, Martin SS,
JournalCancer Res
PubMed ID20924103
Epithelial-to-mesenchymal transition (EMT) is associated with increased breast tumor metastasis; however, the specific mechanisms by which EMT promotes metastasis remain somewhat unclear. Despite the importance of cytoskeletal dynamics during both EMT and metastasis, very few current studies examine the cytoskeleton of detached and circulating tumor cells. Specific posttranslational a-tubulin modifications ... More
Quantitative analysis of the protein corona on FePt nanoparticles formed by transferrin binding.
Nanoparticles are finding a rapidly expanding range of applications in research and technology, finally entering our daily life in medical, cosmetic or food products. Their ability to invade all regions of an organism including cells and cellular organelles offers new strategies for medical diagnosis and therapy (nanomedicine), but their safe ... More
The influence of neuroactive steroid lipophilicity on GABAA receptor modulation: evidence for a low-affinity interaction.
AuthorsChisari M, Eisenman LN, Krishnan K, Bandyopadhyaya AK, Wang C, Taylor A, Benz A, Covey DF, Zorumski CF, Mennerick S,
JournalJ Neurophysiol
PubMed ID19553485
Anesthetic steroids with actions at gamma-aminobutyric acid type A receptors (GABA(A)Rs) may access transmembrane domain binding site(s) directly from the plasma cell membrane. Accordingly, the effective concentration in lipid phase and the ability of the steroid to meet pharmacophore requirements for activity will both contribute to observed steady-state potency. Furthermore, ... More
Three-dimensional structured illumination microscopy of liver sinusoidal endothelial cell fenestrations.
Fenestrations are pores in liver sinusoidal endothelial cells that filter substrates and debris between the blood and hepatocytes. Fenestrations have significant roles in aging and the regulation of lipoproteins. However their small size (<200 nm) has prohibited any functional analysis by light microscopy. We employed structured illumination light microscopy to ... More
Adipocyte Piezo1 mediates obesogenic adipogenesis through the FGF1/FGFR1 signaling pathway in mice.
Authors
JournalNat Commun
PubMed ID32385276
The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis.